Development of Leishmania (Mundinia) in guinea pigs
BACKGROUND: Leishmaniasis is a human and animal disease caused by parasites of the genus Leishmania, which is now divided into four subgenera, Leishmania, Viannia, Sauroleishmania and Mundinia. Subgenus Mundinia, established in 2016, is geographically widely dispersed, its distribution covers all co...
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ftpubmed:oai:pubmedcentral.nih.gov:7140393 2023-05-15T13:47:07+02:00 Development of Leishmania (Mundinia) in guinea pigs Becvar, Tomas Siriyasatien, Padet Bates, Paul Volf, Petr Sádlová, Jovana 2020-04-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140393/ http://www.ncbi.nlm.nih.gov/pubmed/32268916 https://doi.org/10.1186/s13071-020-04039-9 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140393/ http://www.ncbi.nlm.nih.gov/pubmed/32268916 http://dx.doi.org/10.1186/s13071-020-04039-9 © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. CC0 PDM CC-BY Research Text 2020 ftpubmed https://doi.org/10.1186/s13071-020-04039-9 2020-04-19T00:21:59Z BACKGROUND: Leishmaniasis is a human and animal disease caused by parasites of the genus Leishmania, which is now divided into four subgenera, Leishmania, Viannia, Sauroleishmania and Mundinia. Subgenus Mundinia, established in 2016, is geographically widely dispersed, its distribution covers all continents, except Antarctica. It consists of 5 species; L. enriettii and L. macropodum are parasites of wild mammals while L. martiniquensis, L. orientalis and an unnamed Leishmania sp. from Ghana are infectious to humans. There is very little information on natural reservoir hosts and vectors for any Mundinia species. METHODS: Experimental infections of guinea pigs with all five Mundinia species were performed. Animals were injected intradermally with 10(7) culture-derived promastigotes into both ear pinnae. The courses of infections were monitored weekly; xenodiagnoses were performed at weeks 4 and 8 post-infection using Lutzomyia migonei. The distribution of parasites in different tissues was determined post-mortem by conventional PCR. RESULTS: No significant differences in weight were observed between infected animals and the control group. Animals infected with L. enriettii developed temporary lesions at the site of inoculation and were infectious to Lu. migonei in xenodiagnoses. Animals infected with L. martiniquensis and L. orientalis developed temporary erythema and dry lesions at the site of inoculation, respectively, but were not infectious to sand flies. Guinea pigs infected by L. macropodum and Leishmania sp. from Ghana showed no signs of infection during experiments, were not infectious to sand flies and leishmanial DNA was not detected in their tissue samples at the end of experiments at week 12 post-inoculation. CONCLUSIONS: According to our results, guinea pigs are not an appropriate model organism for studying Mundinia species other than L. enriettii. We suggest that for better understanding of L. (Mundinia) biology it is necessary to focus on other model organisms. [Image: see text] Text Antarc* Antarctica PubMed Central (PMC) Parasites & Vectors 13 1 |
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Research Becvar, Tomas Siriyasatien, Padet Bates, Paul Volf, Petr Sádlová, Jovana Development of Leishmania (Mundinia) in guinea pigs |
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BACKGROUND: Leishmaniasis is a human and animal disease caused by parasites of the genus Leishmania, which is now divided into four subgenera, Leishmania, Viannia, Sauroleishmania and Mundinia. Subgenus Mundinia, established in 2016, is geographically widely dispersed, its distribution covers all continents, except Antarctica. It consists of 5 species; L. enriettii and L. macropodum are parasites of wild mammals while L. martiniquensis, L. orientalis and an unnamed Leishmania sp. from Ghana are infectious to humans. There is very little information on natural reservoir hosts and vectors for any Mundinia species. METHODS: Experimental infections of guinea pigs with all five Mundinia species were performed. Animals were injected intradermally with 10(7) culture-derived promastigotes into both ear pinnae. The courses of infections were monitored weekly; xenodiagnoses were performed at weeks 4 and 8 post-infection using Lutzomyia migonei. The distribution of parasites in different tissues was determined post-mortem by conventional PCR. RESULTS: No significant differences in weight were observed between infected animals and the control group. Animals infected with L. enriettii developed temporary lesions at the site of inoculation and were infectious to Lu. migonei in xenodiagnoses. Animals infected with L. martiniquensis and L. orientalis developed temporary erythema and dry lesions at the site of inoculation, respectively, but were not infectious to sand flies. Guinea pigs infected by L. macropodum and Leishmania sp. from Ghana showed no signs of infection during experiments, were not infectious to sand flies and leishmanial DNA was not detected in their tissue samples at the end of experiments at week 12 post-inoculation. CONCLUSIONS: According to our results, guinea pigs are not an appropriate model organism for studying Mundinia species other than L. enriettii. We suggest that for better understanding of L. (Mundinia) biology it is necessary to focus on other model organisms. [Image: see text] |
format |
Text |
author |
Becvar, Tomas Siriyasatien, Padet Bates, Paul Volf, Petr Sádlová, Jovana |
author_facet |
Becvar, Tomas Siriyasatien, Padet Bates, Paul Volf, Petr Sádlová, Jovana |
author_sort |
Becvar, Tomas |
title |
Development of Leishmania (Mundinia) in guinea pigs |
title_short |
Development of Leishmania (Mundinia) in guinea pigs |
title_full |
Development of Leishmania (Mundinia) in guinea pigs |
title_fullStr |
Development of Leishmania (Mundinia) in guinea pigs |
title_full_unstemmed |
Development of Leishmania (Mundinia) in guinea pigs |
title_sort |
development of leishmania (mundinia) in guinea pigs |
publisher |
BioMed Central |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140393/ http://www.ncbi.nlm.nih.gov/pubmed/32268916 https://doi.org/10.1186/s13071-020-04039-9 |
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Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140393/ http://www.ncbi.nlm.nih.gov/pubmed/32268916 http://dx.doi.org/10.1186/s13071-020-04039-9 |
op_rights |
© The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
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CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s13071-020-04039-9 |
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