Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells
Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from fl...
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ftpubmed:oai:pubmedcentral.nih.gov:7128638 2023-05-15T18:15:49+02:00 Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells Zhu, Rong Wang, Jun Lei, Xiao-Ying Gui, Jian-Fang Zhang, Qi-Ya 2013-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128638/ http://www.ncbi.nlm.nih.gov/pubmed/23850425 https://doi.org/10.1016/j.fsi.2013.07.002 en eng Elsevier Ltd. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128638/ http://www.ncbi.nlm.nih.gov/pubmed/23850425 http://dx.doi.org/10.1016/j.fsi.2013.07.002 Copyright © 2013 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. Article Text 2013 ftpubmed https://doi.org/10.1016/j.fsi.2013.07.002 2020-04-12T00:46:58Z Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. Text Scophthalmus maximus PubMed Central (PMC) Fish & Shellfish Immunology 35 3 918 926 |
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Article Zhu, Rong Wang, Jun Lei, Xiao-Ying Gui, Jian-Fang Zhang, Qi-Ya Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells |
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Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. |
format |
Text |
author |
Zhu, Rong Wang, Jun Lei, Xiao-Ying Gui, Jian-Fang Zhang, Qi-Ya |
author_facet |
Zhu, Rong Wang, Jun Lei, Xiao-Ying Gui, Jian-Fang Zhang, Qi-Ya |
author_sort |
Zhu, Rong |
title |
Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells |
title_short |
Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells |
title_full |
Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells |
title_fullStr |
Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells |
title_full_unstemmed |
Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells |
title_sort |
evidence for paralichthys olivaceus ifitm1 antiviral effect by impeding viral entry into target cells |
publisher |
Elsevier Ltd. |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128638/ http://www.ncbi.nlm.nih.gov/pubmed/23850425 https://doi.org/10.1016/j.fsi.2013.07.002 |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128638/ http://www.ncbi.nlm.nih.gov/pubmed/23850425 http://dx.doi.org/10.1016/j.fsi.2013.07.002 |
op_rights |
Copyright © 2013 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
op_doi |
https://doi.org/10.1016/j.fsi.2013.07.002 |
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Fish & Shellfish Immunology |
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35 |
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3 |
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918 |
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926 |
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1766189045158772736 |