Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua)
Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of trypsin ZT biochemistry may give insight...
Published in: | International Journal of Biological Macromolecules |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
The Authors. Published by Elsevier B.V.
2019
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112495/ http://www.ncbi.nlm.nih.gov/pubmed/30550824 https://doi.org/10.1016/j.ijbiomac.2018.12.099 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:7112495 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:7112495 2023-05-15T15:26:58+02:00 Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua) Sandholt, Gunnar B. Stefansson, Bjarki Scheving, Reynir Gudmundsdottir, Ágústa 2019-03-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112495/ http://www.ncbi.nlm.nih.gov/pubmed/30550824 https://doi.org/10.1016/j.ijbiomac.2018.12.099 en eng The Authors. Published by Elsevier B.V. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112495/ http://www.ncbi.nlm.nih.gov/pubmed/30550824 http://dx.doi.org/10.1016/j.ijbiomac.2018.12.099 © 2018 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. Article Text 2019 ftpubmed https://doi.org/10.1016/j.ijbiomac.2018.12.099 2020-04-05T00:58:34Z Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of trypsin ZT biochemistry may give insight into its physiological role as well as its potential use within the biotechnology sector. Stability is an important factor when it comes to practical applications of enzymes. Compared to trypsin I, trypsin ZT shows differences in pH and heat stability, sensitivity to inhibitors and sub-site substrate specificity as shown by multiplex substrate profiling analysis. Based on the analysis, trypsin ZT cleaved at arginine and lysine as other trypsins. Furthermore, trypsin ZT is better than trypsin I in cleaving peptides containing several consecutive positively charged residues. Lysine- and arginine-rich amino acid sequences are frequently found in human viral proteins. Thus, trypsin ZT may be effective in inactivating human and fish viruses implying a possible role for the enzyme in the natural defence of Atlantic cod. The results from this study can lead to multiple practical applications of trypsin ZT. Text atlantic cod Gadus morhua PubMed Central (PMC) International Journal of Biological Macromolecules 125 847 855 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Sandholt, Gunnar B. Stefansson, Bjarki Scheving, Reynir Gudmundsdottir, Ágústa Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua) |
topic_facet |
Article |
description |
Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of trypsin ZT biochemistry may give insight into its physiological role as well as its potential use within the biotechnology sector. Stability is an important factor when it comes to practical applications of enzymes. Compared to trypsin I, trypsin ZT shows differences in pH and heat stability, sensitivity to inhibitors and sub-site substrate specificity as shown by multiplex substrate profiling analysis. Based on the analysis, trypsin ZT cleaved at arginine and lysine as other trypsins. Furthermore, trypsin ZT is better than trypsin I in cleaving peptides containing several consecutive positively charged residues. Lysine- and arginine-rich amino acid sequences are frequently found in human viral proteins. Thus, trypsin ZT may be effective in inactivating human and fish viruses implying a possible role for the enzyme in the natural defence of Atlantic cod. The results from this study can lead to multiple practical applications of trypsin ZT. |
format |
Text |
author |
Sandholt, Gunnar B. Stefansson, Bjarki Scheving, Reynir Gudmundsdottir, Ágústa |
author_facet |
Sandholt, Gunnar B. Stefansson, Bjarki Scheving, Reynir Gudmundsdottir, Ágústa |
author_sort |
Sandholt, Gunnar B. |
title |
Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua) |
title_short |
Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua) |
title_full |
Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua) |
title_fullStr |
Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua) |
title_full_unstemmed |
Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua) |
title_sort |
biochemical characterization of a native group iii trypsin zt from atlantic cod (gadus morhua) |
publisher |
The Authors. Published by Elsevier B.V. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112495/ http://www.ncbi.nlm.nih.gov/pubmed/30550824 https://doi.org/10.1016/j.ijbiomac.2018.12.099 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112495/ http://www.ncbi.nlm.nih.gov/pubmed/30550824 http://dx.doi.org/10.1016/j.ijbiomac.2018.12.099 |
op_rights |
© 2018 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
op_doi |
https://doi.org/10.1016/j.ijbiomac.2018.12.099 |
container_title |
International Journal of Biological Macromolecules |
container_volume |
125 |
container_start_page |
847 |
op_container_end_page |
855 |
_version_ |
1766357422271627264 |