Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide()
Cathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions. In this study, Paralichthys olivaceus cathepsin B (PoCatB) cDNA was isolated from flounder embryonic cells (FEC) treated with UV-inactivated grass carp hemorrhage virus (GCHV) and subseq...
Published in: | Fish & Shellfish Immunology |
---|---|
Main Authors: | , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Elsevier Ltd.
2008
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111675/ http://www.ncbi.nlm.nih.gov/pubmed/18755278 https://doi.org/10.1016/j.fsi.2008.07.018 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:7111675 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:7111675 2023-05-15T18:15:50+02:00 Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide() Zhang, Fu-Tie Zhang, Yi-Bing Chen, Yu-Dong Zhu, Rong Dong, Cai-Wen Li, Yang-Yang Zhang, Qi-Ya Gui, Jian-Fang 2008-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111675/ http://www.ncbi.nlm.nih.gov/pubmed/18755278 https://doi.org/10.1016/j.fsi.2008.07.018 en eng Elsevier Ltd. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111675/ http://www.ncbi.nlm.nih.gov/pubmed/18755278 http://dx.doi.org/10.1016/j.fsi.2008.07.018 Copyright © 2008 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. Article Text 2008 ftpubmed https://doi.org/10.1016/j.fsi.2008.07.018 2020-04-05T00:56:55Z Cathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions. In this study, Paralichthys olivaceus cathepsin B (PoCatB) cDNA was isolated from flounder embryonic cells (FEC) treated with UV-inactivated grass carp hemorrhage virus (GCHV) and subsequently identified as a virally induced gene. The full length cDNA of PoCatB is 1801 bp encoding 330-amino acids. The deduced protein has high homology to all known cathepsin B proteins, containing an N-terminal signal peptide, cysteine protease active sites, the occluding loop segment and a glycosylation site, all of which are conserved in the cathepsin B family. PoCatB transcription of FEC cells could be induced by turbot (Scophthalmus maximus) rhabdovirus (SMRV), UV-inactivated SMRV, UV-inactivated GCHV, poly I:C or lipopolysaccharide (LPS), and SMRV or poly I:C was revealed to be most effective among the five inducers. In normal flounder, PoCatB mRNA was detectable in all examined tissues. Moreover, SMRV infection could result in significant upregulation of PoCatB mRNA, predominantly in spleen, head kidney, posterior kidney, intestine, gill and muscle with 18.2, 10.9, 24.7, 12, 31.5 and 18 fold increases at 72 h post-infection respectively. These results provided the first evidence for the transcriptional induction of cathepsin B in fish by virus and LPS, indicating existence of a novel function in viral defense. Text Scophthalmus maximus Turbot PubMed Central (PMC) Fish & Shellfish Immunology 25 5 542 549 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Zhang, Fu-Tie Zhang, Yi-Bing Chen, Yu-Dong Zhu, Rong Dong, Cai-Wen Li, Yang-Yang Zhang, Qi-Ya Gui, Jian-Fang Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide() |
topic_facet |
Article |
description |
Cathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions. In this study, Paralichthys olivaceus cathepsin B (PoCatB) cDNA was isolated from flounder embryonic cells (FEC) treated with UV-inactivated grass carp hemorrhage virus (GCHV) and subsequently identified as a virally induced gene. The full length cDNA of PoCatB is 1801 bp encoding 330-amino acids. The deduced protein has high homology to all known cathepsin B proteins, containing an N-terminal signal peptide, cysteine protease active sites, the occluding loop segment and a glycosylation site, all of which are conserved in the cathepsin B family. PoCatB transcription of FEC cells could be induced by turbot (Scophthalmus maximus) rhabdovirus (SMRV), UV-inactivated SMRV, UV-inactivated GCHV, poly I:C or lipopolysaccharide (LPS), and SMRV or poly I:C was revealed to be most effective among the five inducers. In normal flounder, PoCatB mRNA was detectable in all examined tissues. Moreover, SMRV infection could result in significant upregulation of PoCatB mRNA, predominantly in spleen, head kidney, posterior kidney, intestine, gill and muscle with 18.2, 10.9, 24.7, 12, 31.5 and 18 fold increases at 72 h post-infection respectively. These results provided the first evidence for the transcriptional induction of cathepsin B in fish by virus and LPS, indicating existence of a novel function in viral defense. |
format |
Text |
author |
Zhang, Fu-Tie Zhang, Yi-Bing Chen, Yu-Dong Zhu, Rong Dong, Cai-Wen Li, Yang-Yang Zhang, Qi-Ya Gui, Jian-Fang |
author_facet |
Zhang, Fu-Tie Zhang, Yi-Bing Chen, Yu-Dong Zhu, Rong Dong, Cai-Wen Li, Yang-Yang Zhang, Qi-Ya Gui, Jian-Fang |
author_sort |
Zhang, Fu-Tie |
title |
Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide() |
title_short |
Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide() |
title_full |
Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide() |
title_fullStr |
Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide() |
title_full_unstemmed |
Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide() |
title_sort |
expressional induction of paralichthys olivaceus cathepsin b gene in response to virus, poly i:c and lipopolysaccharide() |
publisher |
Elsevier Ltd. |
publishDate |
2008 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111675/ http://www.ncbi.nlm.nih.gov/pubmed/18755278 https://doi.org/10.1016/j.fsi.2008.07.018 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111675/ http://www.ncbi.nlm.nih.gov/pubmed/18755278 http://dx.doi.org/10.1016/j.fsi.2008.07.018 |
op_rights |
Copyright © 2008 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
op_doi |
https://doi.org/10.1016/j.fsi.2008.07.018 |
container_title |
Fish & Shellfish Immunology |
container_volume |
25 |
container_issue |
5 |
container_start_page |
542 |
op_container_end_page |
549 |
_version_ |
1766189063415529472 |