QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs()
BACKGROUND: Nucleic acid preparation from a variety of clinical specimens requires efficient target recovery and amplification inhibitor removal and is critical for successful molecular diagnosis. The QIAamp MinElute Virus kit (Qiagen Inc., Valencia, CA) was compared to the two existing methods curr...
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ftpubmed:oai:pubmedcentral.nih.gov:7108269 2023-05-15T17:53:55+02:00 QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs() Sefers, Susan E. Rickmyre, Jamie Blackman, Amondrea Li, Haijing Edwards, Kathryn Tang, Yi-Wei 2006-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108269/ http://www.ncbi.nlm.nih.gov/pubmed/16039902 https://doi.org/10.1016/j.jcv.2005.05.011 en eng Elsevier B.V. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108269/ http://www.ncbi.nlm.nih.gov/pubmed/16039902 http://dx.doi.org/10.1016/j.jcv.2005.05.011 Copyright © 2005 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. Article Text 2006 ftpubmed https://doi.org/10.1016/j.jcv.2005.05.011 2020-04-05T00:52:20Z BACKGROUND: Nucleic acid preparation from a variety of clinical specimens requires efficient target recovery and amplification inhibitor removal and is critical for successful molecular diagnosis. The QIAamp MinElute Virus kit (Qiagen Inc., Valencia, CA) was compared to the two existing methods currently used in our laboratory, IsoQuick (Orca Research Inc., Bothell, WA) for DNA extraction and RNAzol B (Leedo Laboratories Inc., Houston, TX) for RNA extraction, of viral nucleic acids. STUDY DESIGN: A total of 150 clinical specimens, including cerebrospinal fluid (CSF) and nasopharyngeal swabs (NPS), were used to determine the extraction efficiency of the MinElute compared to the other two methods. Nucleic acid recovery, hands-on time, turn-around-time and cost were compared across all kits. RESULTS: There was complete concordance between the MinElute and IsoQuick/RNAzol kits when herpes simplex virus (HSV), Epstein–Barr virus (EBV), varicella-zoster virus (VZV), influenza A virus or enteroviruses were detected using a colorimetric microtiter plate PCR system. The kits were equivalent in their ability to detect either DNA or RNA with superior ability to recover a high quality and quantity of RNA. With the potential to process larger specimen volumes, the MinElute kit can significantly shorten processing time from 2 h to 50–55 min. CONCLUSIONS: Although relatively high test kit costs were noted, the MinElute kit provides another rapid and user-friendly specimen processing tool in the diagnostic molecular microbiology laboratory. Text Orca PubMed Central (PMC) Journal of Clinical Virology 35 2 141 146 |
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Article Sefers, Susan E. Rickmyre, Jamie Blackman, Amondrea Li, Haijing Edwards, Kathryn Tang, Yi-Wei QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs() |
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BACKGROUND: Nucleic acid preparation from a variety of clinical specimens requires efficient target recovery and amplification inhibitor removal and is critical for successful molecular diagnosis. The QIAamp MinElute Virus kit (Qiagen Inc., Valencia, CA) was compared to the two existing methods currently used in our laboratory, IsoQuick (Orca Research Inc., Bothell, WA) for DNA extraction and RNAzol B (Leedo Laboratories Inc., Houston, TX) for RNA extraction, of viral nucleic acids. STUDY DESIGN: A total of 150 clinical specimens, including cerebrospinal fluid (CSF) and nasopharyngeal swabs (NPS), were used to determine the extraction efficiency of the MinElute compared to the other two methods. Nucleic acid recovery, hands-on time, turn-around-time and cost were compared across all kits. RESULTS: There was complete concordance between the MinElute and IsoQuick/RNAzol kits when herpes simplex virus (HSV), Epstein–Barr virus (EBV), varicella-zoster virus (VZV), influenza A virus or enteroviruses were detected using a colorimetric microtiter plate PCR system. The kits were equivalent in their ability to detect either DNA or RNA with superior ability to recover a high quality and quantity of RNA. With the potential to process larger specimen volumes, the MinElute kit can significantly shorten processing time from 2 h to 50–55 min. CONCLUSIONS: Although relatively high test kit costs were noted, the MinElute kit provides another rapid and user-friendly specimen processing tool in the diagnostic molecular microbiology laboratory. |
format |
Text |
author |
Sefers, Susan E. Rickmyre, Jamie Blackman, Amondrea Li, Haijing Edwards, Kathryn Tang, Yi-Wei |
author_facet |
Sefers, Susan E. Rickmyre, Jamie Blackman, Amondrea Li, Haijing Edwards, Kathryn Tang, Yi-Wei |
author_sort |
Sefers, Susan E. |
title |
QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs() |
title_short |
QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs() |
title_full |
QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs() |
title_fullStr |
QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs() |
title_full_unstemmed |
QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs() |
title_sort |
qiaamp minelute virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs() |
publisher |
Elsevier B.V. |
publishDate |
2006 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108269/ http://www.ncbi.nlm.nih.gov/pubmed/16039902 https://doi.org/10.1016/j.jcv.2005.05.011 |
genre |
Orca |
genre_facet |
Orca |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108269/ http://www.ncbi.nlm.nih.gov/pubmed/16039902 http://dx.doi.org/10.1016/j.jcv.2005.05.011 |
op_rights |
Copyright © 2005 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
op_doi |
https://doi.org/10.1016/j.jcv.2005.05.011 |
container_title |
Journal of Clinical Virology |
container_volume |
35 |
container_issue |
2 |
container_start_page |
141 |
op_container_end_page |
146 |
_version_ |
1766161625295880192 |