Lack of evidence of paratuberculosis in wild canids from Southwestern Europe
Wild carnivores are at the top of the trophic chain. They are predators and carrion consumers, and thus, prone to come in contact with disease agents contaminating the environment or infecting live or dead animals. We hypothesized that wild canids could be used as sentinels for the detection of regi...
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ftpubmed:oai:pubmedcentral.nih.gov:7088163 2023-05-15T15:50:27+02:00 Lack of evidence of paratuberculosis in wild canids from Southwestern Europe Sobrino, Raquel Aurtenetxe, O. Carta, Tania Mamian, L. Gerrikagoitia, X. Balseiro, A. Oleaga, A. Sevilla, I. A. Barral, M. Garrido, J. M. Gortazar, Christian 2011-01-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088163/ https://doi.org/10.1007/s10344-010-0490-x en eng Springer-Verlag http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088163/ http://dx.doi.org/10.1007/s10344-010-0490-x © Springer-Verlag 2011 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. Short Communication Text 2011 ftpubmed https://doi.org/10.1007/s10344-010-0490-x 2020-03-29T01:45:35Z Wild carnivores are at the top of the trophic chain. They are predators and carrion consumers, and thus, prone to come in contact with disease agents contaminating the environment or infecting live or dead animals. We hypothesized that wild canids could be used as sentinels for the detection of regions with higher Mycobacterium avium paratuberculosis (MAP) prevalence in wild and domestic animals. To test this hypothesis, we set up an ELISA to test 262 wolf (Canis lupus) and fox (Vulpes vulpes) sera for MAP-specific antibodies and processed a subset of samples for culture (n = 61), MAP-specific PCR (15) and histopathology (14). In wolves, the optical density (OD) values in the ELISA were continuously distributed. Ten fox sera (4%) had OD readings of over twice the mean, suggesting contact with mycobacteria. However, all samples tested by PCR were negative for both IS900 and ISMAP02 sequences, and samples cultured for MAP yielded no growth. No visible paratuberculosis or tuberculosis-compatible lesions were recorded. On histopathological examination, no lesions compatible with mycobacterial diseases were observed. These results suggest that wild canids show little or no evidence of paratuberculosis and are unlikely to be useful sentinels for the detection of MAP in Southwestern Europe. Text Canis lupus PubMed Central (PMC) European Journal of Wildlife Research 57 3 683 688 |
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English |
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Short Communication |
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Short Communication Sobrino, Raquel Aurtenetxe, O. Carta, Tania Mamian, L. Gerrikagoitia, X. Balseiro, A. Oleaga, A. Sevilla, I. A. Barral, M. Garrido, J. M. Gortazar, Christian Lack of evidence of paratuberculosis in wild canids from Southwestern Europe |
topic_facet |
Short Communication |
description |
Wild carnivores are at the top of the trophic chain. They are predators and carrion consumers, and thus, prone to come in contact with disease agents contaminating the environment or infecting live or dead animals. We hypothesized that wild canids could be used as sentinels for the detection of regions with higher Mycobacterium avium paratuberculosis (MAP) prevalence in wild and domestic animals. To test this hypothesis, we set up an ELISA to test 262 wolf (Canis lupus) and fox (Vulpes vulpes) sera for MAP-specific antibodies and processed a subset of samples for culture (n = 61), MAP-specific PCR (15) and histopathology (14). In wolves, the optical density (OD) values in the ELISA were continuously distributed. Ten fox sera (4%) had OD readings of over twice the mean, suggesting contact with mycobacteria. However, all samples tested by PCR were negative for both IS900 and ISMAP02 sequences, and samples cultured for MAP yielded no growth. No visible paratuberculosis or tuberculosis-compatible lesions were recorded. On histopathological examination, no lesions compatible with mycobacterial diseases were observed. These results suggest that wild canids show little or no evidence of paratuberculosis and are unlikely to be useful sentinels for the detection of MAP in Southwestern Europe. |
format |
Text |
author |
Sobrino, Raquel Aurtenetxe, O. Carta, Tania Mamian, L. Gerrikagoitia, X. Balseiro, A. Oleaga, A. Sevilla, I. A. Barral, M. Garrido, J. M. Gortazar, Christian |
author_facet |
Sobrino, Raquel Aurtenetxe, O. Carta, Tania Mamian, L. Gerrikagoitia, X. Balseiro, A. Oleaga, A. Sevilla, I. A. Barral, M. Garrido, J. M. Gortazar, Christian |
author_sort |
Sobrino, Raquel |
title |
Lack of evidence of paratuberculosis in wild canids from Southwestern Europe |
title_short |
Lack of evidence of paratuberculosis in wild canids from Southwestern Europe |
title_full |
Lack of evidence of paratuberculosis in wild canids from Southwestern Europe |
title_fullStr |
Lack of evidence of paratuberculosis in wild canids from Southwestern Europe |
title_full_unstemmed |
Lack of evidence of paratuberculosis in wild canids from Southwestern Europe |
title_sort |
lack of evidence of paratuberculosis in wild canids from southwestern europe |
publisher |
Springer-Verlag |
publishDate |
2011 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088163/ https://doi.org/10.1007/s10344-010-0490-x |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088163/ http://dx.doi.org/10.1007/s10344-010-0490-x |
op_rights |
© Springer-Verlag 2011 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
op_doi |
https://doi.org/10.1007/s10344-010-0490-x |
container_title |
European Journal of Wildlife Research |
container_volume |
57 |
container_issue |
3 |
container_start_page |
683 |
op_container_end_page |
688 |
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1766385382184714240 |