Application of Thin-Layer Chromatography-Flame Ionization Detection (TLC-FID) to Total Lipid Quantitation in Mycolic-Acid Synthesizing Rhodococcus and Williamsia Species

• Published in: International Journal of Molecular Sciences
• Main Authors: Nahar, Akhikun, Baker, Anthony L., Nichols, David S., Bowman, John P., Britz, Margaret L.
• Format: Text
• Language: English
• Published: MDPI 2020
• Subjects: Article; Antarctic; Fid; Antarc*
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084869/; http://www.ncbi.nlm.nih.gov/pubmed/32121355; https://doi.org/10.3390/ijms21051670

In addition to cell membrane phospholipids, Actinobacteria in the order Corynebacteriales possess a waxy cell envelope containing mycolic acids (MA). In optimized culture condition, some species can also accumulate high concentrations of intracellular triacylglycerols (TAG), which are a potential so...