Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro
Mollusk shell is composed of two CaCO(3) polymorphs (calcite and aragonite) and an organic matrix that consists of acetic acid- or ethylenediaminetetraacetic acid (EDTA)-soluble and insoluble proteins and other biomolecules (polysaccharides, β-chitin). However, the shell matrix proteins involved in...
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ftpubmed:oai:pubmedcentral.nih.gov:7081993 2023-05-15T15:58:35+02:00 Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro Arroyo-Loranca, Raquel G. Hernandez-Saavedra, Norma Y. Hernandez-Adame, Luis Rivera-Perez, Crisalejandra 2020-03-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081993/ http://www.ncbi.nlm.nih.gov/pubmed/32191756 https://doi.org/10.1371/journal.pone.0230431 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081993/ http://www.ncbi.nlm.nih.gov/pubmed/32191756 http://dx.doi.org/10.1371/journal.pone.0230431 © 2020 Arroyo-Loranca et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2020 ftpubmed https://doi.org/10.1371/journal.pone.0230431 2020-03-29T01:33:33Z Mollusk shell is composed of two CaCO(3) polymorphs (calcite and aragonite) and an organic matrix that consists of acetic acid- or ethylenediaminetetraacetic acid (EDTA)-soluble and insoluble proteins and other biomolecules (polysaccharides, β-chitin). However, the shell matrix proteins involved in nacre formation are not fully known. Thus, the aim of this study was to identify and characterize a novel protein from the acetic acid-insoluble fraction from the shell of Pteria sterna, named in this study as Ps19, to have a better understanding of the biomineralization process. Ps19 biochemical characterization showed that it is a glycoprotein that exhibits calcium- and chitin-binding capabilities. Additionally, it is capable of inducing aragonite plate crystallization in vitro. Ps19 partial peptide sequence showed similarity with other known shell matrix proteins, but it displayed similarity with proteins from Crassostrea gigas, Mizuhopecten yessoensis, Biomphalaria glabrata, Alpysia californica, Lottia gigantea and Elysia chlorotica. The results obtained indicated that Ps19 might play an important role in nacre growth of mollusk shells. Text Crassostrea gigas PubMed Central (PMC) PLOS ONE 15 3 e0230431 |
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Research Article Arroyo-Loranca, Raquel G. Hernandez-Saavedra, Norma Y. Hernandez-Adame, Luis Rivera-Perez, Crisalejandra Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
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Research Article |
description |
Mollusk shell is composed of two CaCO(3) polymorphs (calcite and aragonite) and an organic matrix that consists of acetic acid- or ethylenediaminetetraacetic acid (EDTA)-soluble and insoluble proteins and other biomolecules (polysaccharides, β-chitin). However, the shell matrix proteins involved in nacre formation are not fully known. Thus, the aim of this study was to identify and characterize a novel protein from the acetic acid-insoluble fraction from the shell of Pteria sterna, named in this study as Ps19, to have a better understanding of the biomineralization process. Ps19 biochemical characterization showed that it is a glycoprotein that exhibits calcium- and chitin-binding capabilities. Additionally, it is capable of inducing aragonite plate crystallization in vitro. Ps19 partial peptide sequence showed similarity with other known shell matrix proteins, but it displayed similarity with proteins from Crassostrea gigas, Mizuhopecten yessoensis, Biomphalaria glabrata, Alpysia californica, Lottia gigantea and Elysia chlorotica. The results obtained indicated that Ps19 might play an important role in nacre growth of mollusk shells. |
format |
Text |
author |
Arroyo-Loranca, Raquel G. Hernandez-Saavedra, Norma Y. Hernandez-Adame, Luis Rivera-Perez, Crisalejandra |
author_facet |
Arroyo-Loranca, Raquel G. Hernandez-Saavedra, Norma Y. Hernandez-Adame, Luis Rivera-Perez, Crisalejandra |
author_sort |
Arroyo-Loranca, Raquel G. |
title |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_short |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_full |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_fullStr |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_full_unstemmed |
Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro |
title_sort |
ps19, a novel chitin binding protein from pteria sterna capable to mineralize aragonite plates in vitro |
publisher |
Public Library of Science |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081993/ http://www.ncbi.nlm.nih.gov/pubmed/32191756 https://doi.org/10.1371/journal.pone.0230431 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081993/ http://www.ncbi.nlm.nih.gov/pubmed/32191756 http://dx.doi.org/10.1371/journal.pone.0230431 |
op_rights |
© 2020 Arroyo-Loranca et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0230431 |
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PLOS ONE |
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15 |
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e0230431 |
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