Mid and hindgut transcriptome profiling analysis of Atlantic salmon (Salmon salar) under unpredictable chronic stress
The intestinal epithelium is a selectively permeable barrier for nutrients, electrolytes and water, while maintaining effective protection against pathogens. Combinations of stressors throughout an animal's life, especially in agriculture and aquaculture settings, may affect the regular operati...
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ftpubmed:oai:pubmedcentral.nih.gov:7062075 2023-05-15T15:31:04+02:00 Mid and hindgut transcriptome profiling analysis of Atlantic salmon (Salmon salar) under unpredictable chronic stress Løvmo, Signe Dille Madaro, Angelico Whatmore, Paul Bardal, Tora Ostensen, Mari-Ann Sandve, Simen R. Olsen, Rolf Erik 2020-02-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062075/ https://doi.org/10.1098/rsos.191480 en eng The Royal Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062075/ http://dx.doi.org/10.1098/rsos.191480 © 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. CC-BY Organismal and Evolutionary Biology Text 2020 ftpubmed https://doi.org/10.1098/rsos.191480 2020-04-05T00:32:34Z The intestinal epithelium is a selectively permeable barrier for nutrients, electrolytes and water, while maintaining effective protection against pathogens. Combinations of stressors throughout an animal's life, especially in agriculture and aquaculture settings, may affect the regular operativity of this organ with negative consequences for animal welfare. In the current study, we report the effects of a three-week unpredictable chronic stress (UCS) period on the intestinal morphology and transcriptome response of Atlantic salmon (Salmon salar) parr midgut and hindgut. Midgut and hindgut from both control and UCS fish were collected for histology and RNA-sequencing analysis to identify respective changes in the membrane structures and putative genes and pathways responding to UCS. Histological analysis did not show any significant effect on morphometric parameters. In the midgut, 1030 genes were differentially expressed following UCS, resulting in 279 genes which were involved in 13 metabolic pathways, including tissue repair pathways. In the hindgut, following UCS, 591 differentially expressed genes were detected with 426 downregulated and 165 upregulated. A total of 53 genes were related to three pathways. Downregulated genes include cellular senescence pathways, p53 signalling and cytokine–cytokine receptor pathways. The overall results corroborate that salmon parr were at least partly habituating to the UCS treatment. In midgut, the main upregulation was related to cell growth and repair, while in the hindgut there were indications of the activated apoptotic pathway, reduced cell repair and inhibited immune/anti-inflammatory capacity. This may be the trade-off between habituating to UCS and health resilience. This study suggests possible integrated genetic regulatory mechanisms that are tuned when farmed Atlantic salmon parr attempt to cope with UCS. Text Atlantic salmon PubMed Central (PMC) Royal Society Open Science 7 2 191480 |
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English |
topic |
Organismal and Evolutionary Biology |
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Organismal and Evolutionary Biology Løvmo, Signe Dille Madaro, Angelico Whatmore, Paul Bardal, Tora Ostensen, Mari-Ann Sandve, Simen R. Olsen, Rolf Erik Mid and hindgut transcriptome profiling analysis of Atlantic salmon (Salmon salar) under unpredictable chronic stress |
topic_facet |
Organismal and Evolutionary Biology |
description |
The intestinal epithelium is a selectively permeable barrier for nutrients, electrolytes and water, while maintaining effective protection against pathogens. Combinations of stressors throughout an animal's life, especially in agriculture and aquaculture settings, may affect the regular operativity of this organ with negative consequences for animal welfare. In the current study, we report the effects of a three-week unpredictable chronic stress (UCS) period on the intestinal morphology and transcriptome response of Atlantic salmon (Salmon salar) parr midgut and hindgut. Midgut and hindgut from both control and UCS fish were collected for histology and RNA-sequencing analysis to identify respective changes in the membrane structures and putative genes and pathways responding to UCS. Histological analysis did not show any significant effect on morphometric parameters. In the midgut, 1030 genes were differentially expressed following UCS, resulting in 279 genes which were involved in 13 metabolic pathways, including tissue repair pathways. In the hindgut, following UCS, 591 differentially expressed genes were detected with 426 downregulated and 165 upregulated. A total of 53 genes were related to three pathways. Downregulated genes include cellular senescence pathways, p53 signalling and cytokine–cytokine receptor pathways. The overall results corroborate that salmon parr were at least partly habituating to the UCS treatment. In midgut, the main upregulation was related to cell growth and repair, while in the hindgut there were indications of the activated apoptotic pathway, reduced cell repair and inhibited immune/anti-inflammatory capacity. This may be the trade-off between habituating to UCS and health resilience. This study suggests possible integrated genetic regulatory mechanisms that are tuned when farmed Atlantic salmon parr attempt to cope with UCS. |
format |
Text |
author |
Løvmo, Signe Dille Madaro, Angelico Whatmore, Paul Bardal, Tora Ostensen, Mari-Ann Sandve, Simen R. Olsen, Rolf Erik |
author_facet |
Løvmo, Signe Dille Madaro, Angelico Whatmore, Paul Bardal, Tora Ostensen, Mari-Ann Sandve, Simen R. Olsen, Rolf Erik |
author_sort |
Løvmo, Signe Dille |
title |
Mid and hindgut transcriptome profiling analysis of Atlantic salmon (Salmon salar) under unpredictable chronic stress |
title_short |
Mid and hindgut transcriptome profiling analysis of Atlantic salmon (Salmon salar) under unpredictable chronic stress |
title_full |
Mid and hindgut transcriptome profiling analysis of Atlantic salmon (Salmon salar) under unpredictable chronic stress |
title_fullStr |
Mid and hindgut transcriptome profiling analysis of Atlantic salmon (Salmon salar) under unpredictable chronic stress |
title_full_unstemmed |
Mid and hindgut transcriptome profiling analysis of Atlantic salmon (Salmon salar) under unpredictable chronic stress |
title_sort |
mid and hindgut transcriptome profiling analysis of atlantic salmon (salmon salar) under unpredictable chronic stress |
publisher |
The Royal Society |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062075/ https://doi.org/10.1098/rsos.191480 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062075/ http://dx.doi.org/10.1098/rsos.191480 |
op_rights |
© 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1098/rsos.191480 |
container_title |
Royal Society Open Science |
container_volume |
7 |
container_issue |
2 |
container_start_page |
191480 |
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1766361556578205696 |