Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii
Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding...
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ftpubmed:oai:pubmedcentral.nih.gov:7058685 2023-05-15T14:03:03+02:00 Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii Schwob, Guillaume Cabrol, Léa Poulin, Elie Orlando, Julieta 2020-02-28 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058685/ http://www.ncbi.nlm.nih.gov/pubmed/32184772 https://doi.org/10.3389/fmicb.2020.00308 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058685/ http://www.ncbi.nlm.nih.gov/pubmed/32184772 http://dx.doi.org/10.3389/fmicb.2020.00308 Copyright © 2020 Schwob, Cabrol, Poulin and Orlando. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2020 ftpubmed https://doi.org/10.3389/fmicb.2020.00308 2020-03-22T01:27:00Z Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed. Text Antarc* Antarctic Antarctic Peninsula King George Island PubMed Central (PMC) Antarctic Antarctic Peninsula King George Island The Antarctic Frontiers in Microbiology 11 |
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Microbiology |
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Microbiology Schwob, Guillaume Cabrol, Léa Poulin, Elie Orlando, Julieta Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
topic_facet |
Microbiology |
description |
Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed. |
format |
Text |
author |
Schwob, Guillaume Cabrol, Léa Poulin, Elie Orlando, Julieta |
author_facet |
Schwob, Guillaume Cabrol, Léa Poulin, Elie Orlando, Julieta |
author_sort |
Schwob, Guillaume |
title |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_short |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_full |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_fullStr |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_full_unstemmed |
Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii |
title_sort |
characterization of the gut microbiota of the antarctic heart urchin (spatangoida) abatus agassizii |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058685/ http://www.ncbi.nlm.nih.gov/pubmed/32184772 https://doi.org/10.3389/fmicb.2020.00308 |
geographic |
Antarctic Antarctic Peninsula King George Island The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula King George Island The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula King George Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula King George Island |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058685/ http://www.ncbi.nlm.nih.gov/pubmed/32184772 http://dx.doi.org/10.3389/fmicb.2020.00308 |
op_rights |
Copyright © 2020 Schwob, Cabrol, Poulin and Orlando. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2020.00308 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
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1766273530535608320 |