Risk factors and prevalence of antibodies for Toxoplasma gondii in diaphragmatic fluid in wolverines (Gulo gulo) from the Northwest Territories, Canada
Toxoplasma gondii, a zoonotic food borne parasite that can infect almost all warm-blooded animals including people, and ranks 4th among 24 most significant global foodborne parasites listed by the World Health Organization/United Nations Food and Agriculture Organization (FAO/WHO, 2014). Exposure to...
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ftpubmed:oai:pubmedcentral.nih.gov:7034056 2023-05-15T15:15:08+02:00 Risk factors and prevalence of antibodies for Toxoplasma gondii in diaphragmatic fluid in wolverines (Gulo gulo) from the Northwest Territories, Canada Sharma, Rajnish Parker, Sarah Elkin, Brett Mulders, Robert Branigan, Marsha Pongracz, Jodie Godson, Dale L. Larter, Nicholas C. Jenkins, Emily 2019-04-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034056/ http://www.ncbi.nlm.nih.gov/pubmed/32095625 https://doi.org/10.1016/j.fawpar.2019.e00056 en eng Elsevier http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034056/ http://www.ncbi.nlm.nih.gov/pubmed/32095625 http://dx.doi.org/10.1016/j.fawpar.2019.e00056 © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2019 ftpubmed https://doi.org/10.1016/j.fawpar.2019.e00056 2020-03-01T01:35:03Z Toxoplasma gondii, a zoonotic food borne parasite that can infect almost all warm-blooded animals including people, and ranks 4th among 24 most significant global foodborne parasites listed by the World Health Organization/United Nations Food and Agriculture Organization (FAO/WHO, 2014). Exposure to T. gondii has been reported in wildlife and people in the Canadian North, despite low densities of feline definitive hosts. The ecology of this host-parasite system could be affected by changing climate and landscape in boreal and sub-Arctic regions, and surveillance data are critically needed. Wolverines are an economically and culturally important species in northern Canada due to their valuable fur. Fluid obtained from diaphragmatic muscle of 127 wolverines (Gulo gulo) were tested for antibodies to T. gondii using an enzyme linked immunosorbent assay (ELISA). A seroprevalence of 62% (Confidence Interval (CI): 53–71%) was observed. This result indicates high levels of exposure, likely either through environmental contamination with T. gondii oocysts shed by infected wild felids, or consumption of carcasses/offal of other intermediate hosts containing tissue cysts with bradyzoites in tissues. We examined factors associated with seropositivity, including age, sex, harvest location, harvest location with respect to treeline, and body condition index. Adult (≥2 years) wolverines had 5.2 times higher odds of being sero-positive than juvenile (<1 years) wolverines. The highest seroprevalence was observed in wolverines from Sahtu and South Slave regions. Proportion of sero-positive wolverines harvested above and below the tree line was not significantly different (60% vs 65%). Age was the only significant predictor of T. gondii exposure in wolverines (using logistic regression analysis); further studies should target larger sample sizes. This study is an example of how fluid from diaphragmatic muscle can be used for screening for T. gondii antibodies in wolverines. The diaphragm, commonly collected for screening for ... Text Arctic Gulo gulo Northwest Territories PubMed Central (PMC) Arctic Canada Northwest Territories Food and Waterborne Parasitology 15 e00056 |
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Article Sharma, Rajnish Parker, Sarah Elkin, Brett Mulders, Robert Branigan, Marsha Pongracz, Jodie Godson, Dale L. Larter, Nicholas C. Jenkins, Emily Risk factors and prevalence of antibodies for Toxoplasma gondii in diaphragmatic fluid in wolverines (Gulo gulo) from the Northwest Territories, Canada |
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Article |
description |
Toxoplasma gondii, a zoonotic food borne parasite that can infect almost all warm-blooded animals including people, and ranks 4th among 24 most significant global foodborne parasites listed by the World Health Organization/United Nations Food and Agriculture Organization (FAO/WHO, 2014). Exposure to T. gondii has been reported in wildlife and people in the Canadian North, despite low densities of feline definitive hosts. The ecology of this host-parasite system could be affected by changing climate and landscape in boreal and sub-Arctic regions, and surveillance data are critically needed. Wolverines are an economically and culturally important species in northern Canada due to their valuable fur. Fluid obtained from diaphragmatic muscle of 127 wolverines (Gulo gulo) were tested for antibodies to T. gondii using an enzyme linked immunosorbent assay (ELISA). A seroprevalence of 62% (Confidence Interval (CI): 53–71%) was observed. This result indicates high levels of exposure, likely either through environmental contamination with T. gondii oocysts shed by infected wild felids, or consumption of carcasses/offal of other intermediate hosts containing tissue cysts with bradyzoites in tissues. We examined factors associated with seropositivity, including age, sex, harvest location, harvest location with respect to treeline, and body condition index. Adult (≥2 years) wolverines had 5.2 times higher odds of being sero-positive than juvenile (<1 years) wolverines. The highest seroprevalence was observed in wolverines from Sahtu and South Slave regions. Proportion of sero-positive wolverines harvested above and below the tree line was not significantly different (60% vs 65%). Age was the only significant predictor of T. gondii exposure in wolverines (using logistic regression analysis); further studies should target larger sample sizes. This study is an example of how fluid from diaphragmatic muscle can be used for screening for T. gondii antibodies in wolverines. The diaphragm, commonly collected for screening for ... |
format |
Text |
author |
Sharma, Rajnish Parker, Sarah Elkin, Brett Mulders, Robert Branigan, Marsha Pongracz, Jodie Godson, Dale L. Larter, Nicholas C. Jenkins, Emily |
author_facet |
Sharma, Rajnish Parker, Sarah Elkin, Brett Mulders, Robert Branigan, Marsha Pongracz, Jodie Godson, Dale L. Larter, Nicholas C. Jenkins, Emily |
author_sort |
Sharma, Rajnish |
title |
Risk factors and prevalence of antibodies for Toxoplasma gondii in diaphragmatic fluid in wolverines (Gulo gulo) from the Northwest Territories, Canada |
title_short |
Risk factors and prevalence of antibodies for Toxoplasma gondii in diaphragmatic fluid in wolverines (Gulo gulo) from the Northwest Territories, Canada |
title_full |
Risk factors and prevalence of antibodies for Toxoplasma gondii in diaphragmatic fluid in wolverines (Gulo gulo) from the Northwest Territories, Canada |
title_fullStr |
Risk factors and prevalence of antibodies for Toxoplasma gondii in diaphragmatic fluid in wolverines (Gulo gulo) from the Northwest Territories, Canada |
title_full_unstemmed |
Risk factors and prevalence of antibodies for Toxoplasma gondii in diaphragmatic fluid in wolverines (Gulo gulo) from the Northwest Territories, Canada |
title_sort |
risk factors and prevalence of antibodies for toxoplasma gondii in diaphragmatic fluid in wolverines (gulo gulo) from the northwest territories, canada |
publisher |
Elsevier |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034056/ http://www.ncbi.nlm.nih.gov/pubmed/32095625 https://doi.org/10.1016/j.fawpar.2019.e00056 |
geographic |
Arctic Canada Northwest Territories |
geographic_facet |
Arctic Canada Northwest Territories |
genre |
Arctic Gulo gulo Northwest Territories |
genre_facet |
Arctic Gulo gulo Northwest Territories |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034056/ http://www.ncbi.nlm.nih.gov/pubmed/32095625 http://dx.doi.org/10.1016/j.fawpar.2019.e00056 |
op_rights |
© 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.fawpar.2019.e00056 |
container_title |
Food and Waterborne Parasitology |
container_volume |
15 |
container_start_page |
e00056 |
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1766345511020789760 |