Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla
In the present study, a new hepatic tissue‐origin cell line from European eel Anguilla anguilla has been developed and characterized. This cell line designated EL has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL...
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ftpubmed:oai:pubmedcentral.nih.gov:7028054 2023-05-15T13:27:11+02:00 Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla Zheng, Zaiyu Yang, Jinxian Ge, Junqing Chi, Hongshu Chen, Bin Fang, Qinmei Gong, Hui 2019-12-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028054/ http://www.ncbi.nlm.nih.gov/pubmed/31814207 https://doi.org/10.1002/cbin.11276 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028054/ http://www.ncbi.nlm.nih.gov/pubmed/31814207 http://dx.doi.org/10.1002/cbin.11276 © 2019 The Authors. Cell Biology International published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Research Articles Text 2019 ftpubmed https://doi.org/10.1002/cbin.11276 2020-03-01T01:28:55Z In the present study, a new hepatic tissue‐origin cell line from European eel Anguilla anguilla has been developed and characterized. This cell line designated EL has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast‐like cells, which could survive over 100 days in vitro, and could grow at 15–32°C. The optimum temperature for growth was 27°C. The chromosome analysis revealed a modal diploid karyotype of 2n = 38. The origin of this cell line was confirmed by the 18S recombinant (r)RNA sequencing. The susceptibility test indicated significant cytopathic effects in the EL cells with regard to the Rana grylio virus and the Herpesvirus anguillae. The viral replication was confirmed by transmission electron microscopy and polymerase chain reaction analysis. Following poly (I:C) exposure, the expression levels of the immune‐related molecules interferon regulatory factor‐7 (irf7) and transforming growth factor‐β (TGF‐β) were downregulated in EL cells, whereas the expression levels of the rf3 and the cytochrome P450 (CYP450) were upregulated. All four genes were significantly upregulated following inflammation by lipopolysaccharide (LPS). These data suggested the application of EL cell line for viral identification, as well as for immunodiagnosis and pharmacological targeting. Text Anguilla anguilla PubMed Central (PMC) Cell Biology International 44 3 808 820 |
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Research Articles Zheng, Zaiyu Yang, Jinxian Ge, Junqing Chi, Hongshu Chen, Bin Fang, Qinmei Gong, Hui Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla |
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Research Articles |
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In the present study, a new hepatic tissue‐origin cell line from European eel Anguilla anguilla has been developed and characterized. This cell line designated EL has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast‐like cells, which could survive over 100 days in vitro, and could grow at 15–32°C. The optimum temperature for growth was 27°C. The chromosome analysis revealed a modal diploid karyotype of 2n = 38. The origin of this cell line was confirmed by the 18S recombinant (r)RNA sequencing. The susceptibility test indicated significant cytopathic effects in the EL cells with regard to the Rana grylio virus and the Herpesvirus anguillae. The viral replication was confirmed by transmission electron microscopy and polymerase chain reaction analysis. Following poly (I:C) exposure, the expression levels of the immune‐related molecules interferon regulatory factor‐7 (irf7) and transforming growth factor‐β (TGF‐β) were downregulated in EL cells, whereas the expression levels of the rf3 and the cytochrome P450 (CYP450) were upregulated. All four genes were significantly upregulated following inflammation by lipopolysaccharide (LPS). These data suggested the application of EL cell line for viral identification, as well as for immunodiagnosis and pharmacological targeting. |
format |
Text |
author |
Zheng, Zaiyu Yang, Jinxian Ge, Junqing Chi, Hongshu Chen, Bin Fang, Qinmei Gong, Hui |
author_facet |
Zheng, Zaiyu Yang, Jinxian Ge, Junqing Chi, Hongshu Chen, Bin Fang, Qinmei Gong, Hui |
author_sort |
Zheng, Zaiyu |
title |
Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla |
title_short |
Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla |
title_full |
Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla |
title_fullStr |
Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla |
title_full_unstemmed |
Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla |
title_sort |
development and characterization of a continuous cell line (el) from the liver of european eel anguilla anguilla |
publisher |
John Wiley and Sons Inc. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028054/ http://www.ncbi.nlm.nih.gov/pubmed/31814207 https://doi.org/10.1002/cbin.11276 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028054/ http://www.ncbi.nlm.nih.gov/pubmed/31814207 http://dx.doi.org/10.1002/cbin.11276 |
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© 2019 The Authors. Cell Biology International published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
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CC-BY |
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https://doi.org/10.1002/cbin.11276 |
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Cell Biology International |
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