Continuous purification of Candida antarctica lipase B using 3‐membrane adsorber periodic counter‐current chromatography
Batch chromatography has several disadvantages, such as insufficient utilization of the capacity of the resin, high buffer consumption and discontinuity. Considering the high costs for downstream processing, a continuously working chromatographic system with three membrane adsorber units was designe...
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ftpubmed:oai:pubmedcentral.nih.gov:6999568 2023-05-15T14:01:31+02:00 Continuous purification of Candida antarctica lipase B using 3‐membrane adsorber periodic counter‐current chromatography Brämer, Chantal Schreiber, Sarah Scheper, Thomas Beutel, Sascha 2018-04-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999568/ https://doi.org/10.1002/elsc.201700159 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999568/ http://dx.doi.org/10.1002/elsc.201700159 © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Eng Life Sci Research Articles Text 2018 ftpubmed https://doi.org/10.1002/elsc.201700159 2020-07-05T00:30:38Z Batch chromatography has several disadvantages, such as insufficient utilization of the capacity of the resin, high buffer consumption and discontinuity. Considering the high costs for downstream processing, a continuously working chromatographic system with three membrane adsorber units was designed, tested and put into operation. The basic principle of the setup is periodic counter‐current chromatography (PCCC). The PCCC system was used for capturing and purifying Candida antarctica lipase B (CalB) directly from cell lysate in one single unit operation. The best purification result was achieved by means of anion‐exchange chromatography. The dynamic binding capacity with Sartobind® Q 75 amounted to 4.2 mg (56 g/cm(2)). After transferring the method to the 3MA‐PCCC, 0.22 g CalB (73 U/mg) were obtained from 0.9 L E. coli lysate within 6 h and a recovery of 80%. Compared to the batch process, the productivity could be increased by 36% and the buffer consumption could be reduced by about 20%. Although the purification of CalB from lysate by means of anion‐exchange chromatography was not selective and quantitative using the 3MA‐PCCC device, it could be shown that the concept of the system was successfully implemented and led to a significant improvement of CalB purification. Text Antarc* Antarctica PubMed Central (PMC) Engineering in Life Sciences 18 7 414 424 |
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Research Articles Brämer, Chantal Schreiber, Sarah Scheper, Thomas Beutel, Sascha Continuous purification of Candida antarctica lipase B using 3‐membrane adsorber periodic counter‐current chromatography |
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Batch chromatography has several disadvantages, such as insufficient utilization of the capacity of the resin, high buffer consumption and discontinuity. Considering the high costs for downstream processing, a continuously working chromatographic system with three membrane adsorber units was designed, tested and put into operation. The basic principle of the setup is periodic counter‐current chromatography (PCCC). The PCCC system was used for capturing and purifying Candida antarctica lipase B (CalB) directly from cell lysate in one single unit operation. The best purification result was achieved by means of anion‐exchange chromatography. The dynamic binding capacity with Sartobind® Q 75 amounted to 4.2 mg (56 g/cm(2)). After transferring the method to the 3MA‐PCCC, 0.22 g CalB (73 U/mg) were obtained from 0.9 L E. coli lysate within 6 h and a recovery of 80%. Compared to the batch process, the productivity could be increased by 36% and the buffer consumption could be reduced by about 20%. Although the purification of CalB from lysate by means of anion‐exchange chromatography was not selective and quantitative using the 3MA‐PCCC device, it could be shown that the concept of the system was successfully implemented and led to a significant improvement of CalB purification. |
format |
Text |
author |
Brämer, Chantal Schreiber, Sarah Scheper, Thomas Beutel, Sascha |
author_facet |
Brämer, Chantal Schreiber, Sarah Scheper, Thomas Beutel, Sascha |
author_sort |
Brämer, Chantal |
title |
Continuous purification of Candida antarctica lipase B using 3‐membrane adsorber periodic counter‐current chromatography |
title_short |
Continuous purification of Candida antarctica lipase B using 3‐membrane adsorber periodic counter‐current chromatography |
title_full |
Continuous purification of Candida antarctica lipase B using 3‐membrane adsorber periodic counter‐current chromatography |
title_fullStr |
Continuous purification of Candida antarctica lipase B using 3‐membrane adsorber periodic counter‐current chromatography |
title_full_unstemmed |
Continuous purification of Candida antarctica lipase B using 3‐membrane adsorber periodic counter‐current chromatography |
title_sort |
continuous purification of candida antarctica lipase b using 3‐membrane adsorber periodic counter‐current chromatography |
publisher |
John Wiley and Sons Inc. |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999568/ https://doi.org/10.1002/elsc.201700159 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Eng Life Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999568/ http://dx.doi.org/10.1002/elsc.201700159 |
op_rights |
© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
op_doi |
https://doi.org/10.1002/elsc.201700159 |
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Engineering in Life Sciences |
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18 |
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7 |
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414 |
op_container_end_page |
424 |
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1766271351042080768 |