A continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity
The design of an optimal process is particularly crucial when the reactants deactivate the biocatalyst. The reaction cascades of the chemo‐enzymatic epoxidation where the intermediate peroxy acid is produced by an enzyme are still limited by enzyme inhibition and deactivation by hydrogen peroxide. T...
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ftpubmed:oai:pubmedcentral.nih.gov:6999354 2023-05-15T14:01:31+02:00 A continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity Meyer, Janine Horst, Angelika E. W. Steinhagen, Max Holtmann, Dirk Ansorge‐Schumacher, Marion B. Kraume, Matthias Drews, Anja 2017-05-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999354/ https://doi.org/10.1002/elsc.201600171 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999354/ http://dx.doi.org/10.1002/elsc.201600171 © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Eng Life Sci Research Articles Text 2017 ftpubmed https://doi.org/10.1002/elsc.201600171 2020-07-05T00:30:04Z The design of an optimal process is particularly crucial when the reactants deactivate the biocatalyst. The reaction cascades of the chemo‐enzymatic epoxidation where the intermediate peroxy acid is produced by an enzyme are still limited by enzyme inhibition and deactivation by hydrogen peroxide. To avoid additional effects caused by interfaces (aq/org) and to reduce the process limiting deactivation by the substrate hydrogen peroxide, a single‐phase concept was applied in a fed‐batch and a continuous process (stirred tank), without the commonly applied addition of a carrier solvent. The synthesis of peroxyoctanoic acid catalyzed by Candida antarctica lipase B was chosen as the model reaction. Here, the feasibility of this biocatalytic reaction in a single‐phase system was shown for the first time. The work shows the economic superiority of the continuous process compared to the fed‐batch process. Employing the fed‐batch process reaction rates up to 36 mmol h(−1) per gram(cat), and a maximum yield of 96 % was achieved, but activity dropped quickly. In contrast, continuous operation can maintain long‐term enzyme activity. For the first time, the continuous enzymatic reaction could be performed for 55 h without any loss of activity and with a space‐time yield of 154 mmol L(−1) h(−1), which is three times higher than in the fed‐batch process. The higher catalytic productivity compared to the fed‐batch process (34 vs. 18 g(Prod) g(−1) (cat)) shows the increased enzyme stability in the continuous process. Text Antarc* Antarctica PubMed Central (PMC) Engineering in Life Sciences 17 7 759 767 |
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Research Articles Meyer, Janine Horst, Angelika E. W. Steinhagen, Max Holtmann, Dirk Ansorge‐Schumacher, Marion B. Kraume, Matthias Drews, Anja A continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity |
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Research Articles |
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The design of an optimal process is particularly crucial when the reactants deactivate the biocatalyst. The reaction cascades of the chemo‐enzymatic epoxidation where the intermediate peroxy acid is produced by an enzyme are still limited by enzyme inhibition and deactivation by hydrogen peroxide. To avoid additional effects caused by interfaces (aq/org) and to reduce the process limiting deactivation by the substrate hydrogen peroxide, a single‐phase concept was applied in a fed‐batch and a continuous process (stirred tank), without the commonly applied addition of a carrier solvent. The synthesis of peroxyoctanoic acid catalyzed by Candida antarctica lipase B was chosen as the model reaction. Here, the feasibility of this biocatalytic reaction in a single‐phase system was shown for the first time. The work shows the economic superiority of the continuous process compared to the fed‐batch process. Employing the fed‐batch process reaction rates up to 36 mmol h(−1) per gram(cat), and a maximum yield of 96 % was achieved, but activity dropped quickly. In contrast, continuous operation can maintain long‐term enzyme activity. For the first time, the continuous enzymatic reaction could be performed for 55 h without any loss of activity and with a space‐time yield of 154 mmol L(−1) h(−1), which is three times higher than in the fed‐batch process. The higher catalytic productivity compared to the fed‐batch process (34 vs. 18 g(Prod) g(−1) (cat)) shows the increased enzyme stability in the continuous process. |
format |
Text |
author |
Meyer, Janine Horst, Angelika E. W. Steinhagen, Max Holtmann, Dirk Ansorge‐Schumacher, Marion B. Kraume, Matthias Drews, Anja |
author_facet |
Meyer, Janine Horst, Angelika E. W. Steinhagen, Max Holtmann, Dirk Ansorge‐Schumacher, Marion B. Kraume, Matthias Drews, Anja |
author_sort |
Meyer, Janine |
title |
A continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity |
title_short |
A continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity |
title_full |
A continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity |
title_fullStr |
A continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity |
title_full_unstemmed |
A continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity |
title_sort |
continuous single organic phase process for the lipase catalyzed synthesis of peroxy acids increases productivity |
publisher |
John Wiley and Sons Inc. |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999354/ https://doi.org/10.1002/elsc.201600171 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Eng Life Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999354/ http://dx.doi.org/10.1002/elsc.201600171 |
op_rights |
© 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
op_doi |
https://doi.org/10.1002/elsc.201600171 |
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