Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean

Proteorhodopsin (PR) is discovered from marine bacteria and it has proton pumping activity from inside to outside of the cell using light energy. In general, PR classified into two groups by the maximum absorption spectra. In this study, we isolated the two of a full sequence of opsin homologues by...

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Published in:Scientific Reports
Main Authors: Kim, Se-Hwan, Jung, ByungHoon, Hong, Soon Gyu, Jung, Kwang-Hwan
Format: Text
Language:English
Published: Nature Publishing Group UK 2020
Subjects:
Article
King George Island
North Pole
Antarc*
Antarctica
antartic*
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987182/
http://www.ncbi.nlm.nih.gov/pubmed/31992768
https://doi.org/10.1038/s41598-020-58023-5
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author Kim, Se-Hwan
Jung, ByungHoon
Hong, Soon Gyu
Jung, Kwang-Hwan
author_facet Kim, Se-Hwan
Jung, ByungHoon
Hong, Soon Gyu
Jung, Kwang-Hwan
author_sort Kim, Se-Hwan
collection PubMed Central (PMC)
container_issue 1
container_title Scientific Reports
container_volume 10
description Proteorhodopsin (PR) is discovered from marine bacteria and it has proton pumping activity from inside to outside of the cell using light energy. In general, PR classified into two groups by the maximum absorption spectra. In this study, we isolated the two of a full sequence of opsin homologues by PCR from the seawater sample near King George Island, Antarctica. One was the same sequence as the first reported GPR (Green-light absorbing PR) from Monterey Bay. Another named HSG119 was a newly discovered sequence which shows high sequence similarity with BPR (Blue-light absorbing PR). HSG119 has an absorption maximum at 493 nm with broader spectrum at pH7.0 and it can pump protons out of the cell membrane. Interestingly, it showed a similar temperature dependence to GPR(Y200N) that isolated near the North pole.
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genre Antarc*
Antarctica
antartic*
King George Island
North Pole
genre_facet Antarc*
Antarctica
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North Pole
geographic King George Island
North Pole
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op_doi https://doi.org/10.1038/s41598-020-58023-5
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987182/
http://www.ncbi.nlm.nih.gov/pubmed/31992768
http://dx.doi.org/10.1038/s41598-020-58023-5
op_rights © The Author(s) 2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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spelling ftpubmed:oai:pubmedcentral.nih.gov:6987182 2025-01-16T19:01:21+00:00 Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean Kim, Se-Hwan Jung, ByungHoon Hong, Soon Gyu Jung, Kwang-Hwan 2020-01-28 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987182/ http://www.ncbi.nlm.nih.gov/pubmed/31992768 https://doi.org/10.1038/s41598-020-58023-5 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987182/ http://www.ncbi.nlm.nih.gov/pubmed/31992768 http://dx.doi.org/10.1038/s41598-020-58023-5 © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2020 ftpubmed https://doi.org/10.1038/s41598-020-58023-5 2020-02-09T01:30:18Z Proteorhodopsin (PR) is discovered from marine bacteria and it has proton pumping activity from inside to outside of the cell using light energy. In general, PR classified into two groups by the maximum absorption spectra. In this study, we isolated the two of a full sequence of opsin homologues by PCR from the seawater sample near King George Island, Antarctica. One was the same sequence as the first reported GPR (Green-light absorbing PR) from Monterey Bay. Another named HSG119 was a newly discovered sequence which shows high sequence similarity with BPR (Blue-light absorbing PR). HSG119 has an absorption maximum at 493 nm with broader spectrum at pH7.0 and it can pump protons out of the cell membrane. Interestingly, it showed a similar temperature dependence to GPR(Y200N) that isolated near the North pole. Text Antarc* Antarctica antartic* King George Island North Pole PubMed Central (PMC) King George Island North Pole Scientific Reports 10 1
spellingShingle Article
Kim, Se-Hwan
Jung, ByungHoon
Hong, Soon Gyu
Jung, Kwang-Hwan
Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean
title Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean
title_full Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean
title_fullStr Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean
title_full_unstemmed Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean
title_short Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean
title_sort temperature dependency of proton pumping activity for marine microbial rhodopsin from antartic ocean
topic Article
topic_facet Article
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987182/
http://www.ncbi.nlm.nih.gov/pubmed/31992768
https://doi.org/10.1038/s41598-020-58023-5

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