Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome

BACKGROUND: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has e...

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Published in:BMC Genomics
Main Authors: de Lorgeril, Julien, Petton, Bruno, Lucasson, Aude, Perez, Valérie, Stenger, Pierre-Louis, Dégremont, Lionel, Montagnani, Caroline, Escoubas, Jean-Michel, Haffner, Philippe, Allienne, Jean-François, Leroy, Marc, Lagarde, Franck, Vidal-Dupiol, Jérémie, Gueguen, Yannick, Mitta, Guillaume
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Language:English
Published: BioMed Central 2020
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Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971885/
http://www.ncbi.nlm.nih.gov/pubmed/31959106
https://doi.org/10.1186/s12864-020-6471-x
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spelling ftpubmed:oai:pubmedcentral.nih.gov:6971885 2023-05-15T15:58:42+02:00 Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome de Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Stenger, Pierre-Louis Dégremont, Lionel Montagnani, Caroline Escoubas, Jean-Michel Haffner, Philippe Allienne, Jean-François Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jérémie Gueguen, Yannick Mitta, Guillaume 2020-01-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971885/ http://www.ncbi.nlm.nih.gov/pubmed/31959106 https://doi.org/10.1186/s12864-020-6471-x en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971885/ http://www.ncbi.nlm.nih.gov/pubmed/31959106 http://dx.doi.org/10.1186/s12864-020-6471-x © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Research Article Text 2020 ftpubmed https://doi.org/10.1186/s12864-020-6471-x 2020-02-02T01:26:52Z BACKGROUND: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). RESULTS: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. CONCLUSIONS: We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific BMC Genomics 21 1
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Research Article
spellingShingle Research Article
de Lorgeril, Julien
Petton, Bruno
Lucasson, Aude
Perez, Valérie
Stenger, Pierre-Louis
Dégremont, Lionel
Montagnani, Caroline
Escoubas, Jean-Michel
Haffner, Philippe
Allienne, Jean-François
Leroy, Marc
Lagarde, Franck
Vidal-Dupiol, Jérémie
Gueguen, Yannick
Mitta, Guillaume
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome
topic_facet Research Article
description BACKGROUND: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). RESULTS: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. CONCLUSIONS: We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function.
format Text
author de Lorgeril, Julien
Petton, Bruno
Lucasson, Aude
Perez, Valérie
Stenger, Pierre-Louis
Dégremont, Lionel
Montagnani, Caroline
Escoubas, Jean-Michel
Haffner, Philippe
Allienne, Jean-François
Leroy, Marc
Lagarde, Franck
Vidal-Dupiol, Jérémie
Gueguen, Yannick
Mitta, Guillaume
author_facet de Lorgeril, Julien
Petton, Bruno
Lucasson, Aude
Perez, Valérie
Stenger, Pierre-Louis
Dégremont, Lionel
Montagnani, Caroline
Escoubas, Jean-Michel
Haffner, Philippe
Allienne, Jean-François
Leroy, Marc
Lagarde, Franck
Vidal-Dupiol, Jérémie
Gueguen, Yannick
Mitta, Guillaume
author_sort de Lorgeril, Julien
title Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome
title_short Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome
title_full Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome
title_fullStr Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome
title_full_unstemmed Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome
title_sort differential basal expression of immune genes confers crassostrea gigas resistance to pacific oyster mortality syndrome
publisher BioMed Central
publishDate 2020
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971885/
http://www.ncbi.nlm.nih.gov/pubmed/31959106
https://doi.org/10.1186/s12864-020-6471-x
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971885/
http://www.ncbi.nlm.nih.gov/pubmed/31959106
http://dx.doi.org/10.1186/s12864-020-6471-x
op_rights © The Author(s). 2020
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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container_title BMC Genomics
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