Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line
Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, a systemic infection of salmonid fish species. P. salmonis infects and survives in its host cell, a process that correlates with the expression of virulence factors including components of the type IVB secretion system. To gain f...
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ftpubmed:oai:pubmedcentral.nih.gov:6964531 2023-05-15T15:32:46+02:00 Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line Zúñiga, Alejandro Aravena, Pamela Pulgar, Rodrigo Travisany, Dante Ortiz-Severín, Javiera Chávez, Francisco P. Maass, Alejandro González, Mauricio Cambiazo, Verónica 2020-01-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964531/ https://doi.org/10.3389/fcimb.2019.00426 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964531/ http://dx.doi.org/10.3389/fcimb.2019.00426 Copyright © 2020 Zúñiga, Aravena, Pulgar, Travisany, Ortiz-Severín, Chávez, Maass, González and Cambiazo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Cellular and Infection Microbiology Text 2020 ftpubmed https://doi.org/10.3389/fcimb.2019.00426 2020-02-02T01:20:56Z Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, a systemic infection of salmonid fish species. P. salmonis infects and survives in its host cell, a process that correlates with the expression of virulence factors including components of the type IVB secretion system. To gain further insights into the cellular and molecular mechanism behind the adaptive response of P. salmonis during host infection, we established an in vitro model of infection using the SHK-1 cell line from Atlantic salmon head kidney. The results indicated that in comparison to uninfected SHK-1 cells, infection significantly decreased cell viability after 10 days along with a significant increment of P. salmonis genome equivalents. At that time, the intracellular bacteria were localized within a spacious cytoplasmic vacuole. By using a whole-genome microarray of P. salmonis LF-89, the transcriptome of this bacterium was examined during intracellular growth in the SHK-1 cell line and exponential growth in broth. Transcriptome analysis revealed a global shutdown of translation during P. salmonis intracellular growth and suggested an induction of the stringent response. Accordingly, key genes of the stringent response pathway were up-regulated during intracellular growth as well as at stationary phase bacteria, suggesting a role of the stringent response on bacterial virulence. Our results also reinforce the participation of the Dot/Icm type IVB secretion system during P. salmonis infection and reveals many unexplored genes with potential roles in the adaptation to intracellular growth. Finally, we proposed that intracellular P. salmonis alternates between a replicative phase and a stationary phase in which the stringent response is activated. Text Atlantic salmon PubMed Central (PMC) Frontiers in Cellular and Infection Microbiology 9 |
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Cellular and Infection Microbiology |
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Cellular and Infection Microbiology Zúñiga, Alejandro Aravena, Pamela Pulgar, Rodrigo Travisany, Dante Ortiz-Severín, Javiera Chávez, Francisco P. Maass, Alejandro González, Mauricio Cambiazo, Verónica Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line |
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Cellular and Infection Microbiology |
description |
Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, a systemic infection of salmonid fish species. P. salmonis infects and survives in its host cell, a process that correlates with the expression of virulence factors including components of the type IVB secretion system. To gain further insights into the cellular and molecular mechanism behind the adaptive response of P. salmonis during host infection, we established an in vitro model of infection using the SHK-1 cell line from Atlantic salmon head kidney. The results indicated that in comparison to uninfected SHK-1 cells, infection significantly decreased cell viability after 10 days along with a significant increment of P. salmonis genome equivalents. At that time, the intracellular bacteria were localized within a spacious cytoplasmic vacuole. By using a whole-genome microarray of P. salmonis LF-89, the transcriptome of this bacterium was examined during intracellular growth in the SHK-1 cell line and exponential growth in broth. Transcriptome analysis revealed a global shutdown of translation during P. salmonis intracellular growth and suggested an induction of the stringent response. Accordingly, key genes of the stringent response pathway were up-regulated during intracellular growth as well as at stationary phase bacteria, suggesting a role of the stringent response on bacterial virulence. Our results also reinforce the participation of the Dot/Icm type IVB secretion system during P. salmonis infection and reveals many unexplored genes with potential roles in the adaptation to intracellular growth. Finally, we proposed that intracellular P. salmonis alternates between a replicative phase and a stationary phase in which the stringent response is activated. |
format |
Text |
author |
Zúñiga, Alejandro Aravena, Pamela Pulgar, Rodrigo Travisany, Dante Ortiz-Severín, Javiera Chávez, Francisco P. Maass, Alejandro González, Mauricio Cambiazo, Verónica |
author_facet |
Zúñiga, Alejandro Aravena, Pamela Pulgar, Rodrigo Travisany, Dante Ortiz-Severín, Javiera Chávez, Francisco P. Maass, Alejandro González, Mauricio Cambiazo, Verónica |
author_sort |
Zúñiga, Alejandro |
title |
Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line |
title_short |
Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line |
title_full |
Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line |
title_fullStr |
Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line |
title_full_unstemmed |
Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line |
title_sort |
transcriptomic changes of piscirickettsia salmonis during intracellular growth in a salmon macrophage-like cell line |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964531/ https://doi.org/10.3389/fcimb.2019.00426 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964531/ http://dx.doi.org/10.3389/fcimb.2019.00426 |
op_rights |
Copyright © 2020 Zúñiga, Aravena, Pulgar, Travisany, Ortiz-Severín, Chávez, Maass, González and Cambiazo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fcimb.2019.00426 |
container_title |
Frontiers in Cellular and Infection Microbiology |
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9 |
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1766363250340921344 |