Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease
Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis o...
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ftpubmed:oai:pubmedcentral.nih.gov:6963586 2023-05-15T15:32:28+02:00 Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease Johnson-Mackinnon, Jessica C. Crosbie, Philip B. B. Karlsbakk, Egil Marcos-Lopez, Mar Paley, Richard Nowak, Barbara F. Bridle, Andrew R. 2019-11-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963586/ http://www.ncbi.nlm.nih.gov/pubmed/31752364 https://doi.org/10.3390/pathogens8040244 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963586/ http://www.ncbi.nlm.nih.gov/pubmed/31752364 http://dx.doi.org/10.3390/pathogens8040244 © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2019 ftpubmed https://doi.org/10.3390/pathogens8040244 2020-02-02T01:19:46Z Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to N. perurans with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of N. perurans from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area. Text Atlantic salmon PubMed Central (PMC) Canada Norway Pathogens 8 4 244 |
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Article Johnson-Mackinnon, Jessica C. Crosbie, Philip B. B. Karlsbakk, Egil Marcos-Lopez, Mar Paley, Richard Nowak, Barbara F. Bridle, Andrew R. Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
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Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to N. perurans with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of N. perurans from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area. |
format |
Text |
author |
Johnson-Mackinnon, Jessica C. Crosbie, Philip B. B. Karlsbakk, Egil Marcos-Lopez, Mar Paley, Richard Nowak, Barbara F. Bridle, Andrew R. |
author_facet |
Johnson-Mackinnon, Jessica C. Crosbie, Philip B. B. Karlsbakk, Egil Marcos-Lopez, Mar Paley, Richard Nowak, Barbara F. Bridle, Andrew R. |
author_sort |
Johnson-Mackinnon, Jessica C. |
title |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_short |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_full |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_fullStr |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_full_unstemmed |
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease |
title_sort |
multilocus sequence typing (mlst) and random polymorphic dna (rapd) comparisons of geographic isolates of neoparamoeba perurans, the causative agent of amoebic gill disease |
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MDPI |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963586/ http://www.ncbi.nlm.nih.gov/pubmed/31752364 https://doi.org/10.3390/pathogens8040244 |
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Canada Norway |
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Canada Norway |
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Atlantic salmon |
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Atlantic salmon |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963586/ http://www.ncbi.nlm.nih.gov/pubmed/31752364 http://dx.doi.org/10.3390/pathogens8040244 |
op_rights |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/pathogens8040244 |
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Pathogens |
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