Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum)
PREMISE: Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. METHODS AND RESULTS: Forty‐eight primer pairs were desig...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923708/ http://www.ncbi.nlm.nih.gov/pubmed/31890352 https://doi.org/10.1002/aps3.11306 |
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ftpubmed:oai:pubmedcentral.nih.gov:6923708 2023-05-15T17:46:38+02:00 Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) Sheik, Matthew L. LaBounty, Kitty L. Mitchell, Erika Gillespie, Emily L. 2019-12-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923708/ http://www.ncbi.nlm.nih.gov/pubmed/31890352 https://doi.org/10.1002/aps3.11306 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923708/ http://www.ncbi.nlm.nih.gov/pubmed/31890352 http://dx.doi.org/10.1002/aps3.11306 © 2019 Sheik et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. CC-BY-NC Primer Notes Text 2019 ftpubmed https://doi.org/10.1002/aps3.11306 2020-01-05T01:37:40Z PREMISE: Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. METHODS AND RESULTS: Forty‐eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, populations can be easily discriminated. Cross‐amplification in other Rhododendron subsect. Ledum species shows broad application of the developed markers within this small, well‐supported clade. CONCLUSIONS: These microsatellite markers exhibit significant variability and will be useful in population genetics within R. groenlandicum and for investigation of species boundaries across Rhododendron subsect. Ledum. Text Northwest Territories Alaska PubMed Central (PMC) Canada Northwest Territories Applications in Plant Sciences 7 12 |
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Primer Notes |
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Primer Notes Sheik, Matthew L. LaBounty, Kitty L. Mitchell, Erika Gillespie, Emily L. Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
topic_facet |
Primer Notes |
description |
PREMISE: Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. METHODS AND RESULTS: Forty‐eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, populations can be easily discriminated. Cross‐amplification in other Rhododendron subsect. Ledum species shows broad application of the developed markers within this small, well‐supported clade. CONCLUSIONS: These microsatellite markers exhibit significant variability and will be useful in population genetics within R. groenlandicum and for investigation of species boundaries across Rhododendron subsect. Ledum. |
format |
Text |
author |
Sheik, Matthew L. LaBounty, Kitty L. Mitchell, Erika Gillespie, Emily L. |
author_facet |
Sheik, Matthew L. LaBounty, Kitty L. Mitchell, Erika Gillespie, Emily L. |
author_sort |
Sheik, Matthew L. |
title |
Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
title_short |
Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
title_full |
Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
title_fullStr |
Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
title_full_unstemmed |
Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
title_sort |
fourteen polymorphic microsatellite markers for the widespread labrador tea (rhododendron groenlandicum) |
publisher |
John Wiley and Sons Inc. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923708/ http://www.ncbi.nlm.nih.gov/pubmed/31890352 https://doi.org/10.1002/aps3.11306 |
geographic |
Canada Northwest Territories |
geographic_facet |
Canada Northwest Territories |
genre |
Northwest Territories Alaska |
genre_facet |
Northwest Territories Alaska |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923708/ http://www.ncbi.nlm.nih.gov/pubmed/31890352 http://dx.doi.org/10.1002/aps3.11306 |
op_rights |
© 2019 Sheik et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1002/aps3.11306 |
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Applications in Plant Sciences |
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7 |
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12 |
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