Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis
Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to me...
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ftpubmed:oai:pubmedcentral.nih.gov:6915758 2023-05-15T15:56:35+02:00 Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis Hoettges, Kai F. Henslee, Erin A. Torcal Serrano, Ruth M. Jabr, Rita I. Abdallat, Rula G. Beale, Andrew D. Waheed, Abdul Camelliti, Patrizia Fry, Christopher H. van der Veen, Daan R. Labeed, Fatima H. Hughes, Michael P. 2019-12-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915758/ http://www.ncbi.nlm.nih.gov/pubmed/31844107 https://doi.org/10.1038/s41598-019-55579-9 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915758/ http://www.ncbi.nlm.nih.gov/pubmed/31844107 http://dx.doi.org/10.1038/s41598-019-55579-9 © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2019 ftpubmed https://doi.org/10.1038/s41598-019-55579-9 2019-12-22T01:30:58Z Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r(2) > 0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2–3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. Text Common vole Microtus arvalis PubMed Central (PMC) Scientific Reports 9 1 |
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Article Hoettges, Kai F. Henslee, Erin A. Torcal Serrano, Ruth M. Jabr, Rita I. Abdallat, Rula G. Beale, Andrew D. Waheed, Abdul Camelliti, Patrizia Fry, Christopher H. van der Veen, Daan R. Labeed, Fatima H. Hughes, Michael P. Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
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Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r(2) > 0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2–3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. |
format |
Text |
author |
Hoettges, Kai F. Henslee, Erin A. Torcal Serrano, Ruth M. Jabr, Rita I. Abdallat, Rula G. Beale, Andrew D. Waheed, Abdul Camelliti, Patrizia Fry, Christopher H. van der Veen, Daan R. Labeed, Fatima H. Hughes, Michael P. |
author_facet |
Hoettges, Kai F. Henslee, Erin A. Torcal Serrano, Ruth M. Jabr, Rita I. Abdallat, Rula G. Beale, Andrew D. Waheed, Abdul Camelliti, Patrizia Fry, Christopher H. van der Veen, Daan R. Labeed, Fatima H. Hughes, Michael P. |
author_sort |
Hoettges, Kai F. |
title |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_short |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_full |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_fullStr |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_full_unstemmed |
Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_sort |
ten–second electrophysiology: evaluation of the 3dep platform for high-speed, high-accuracy cell analysis |
publisher |
Nature Publishing Group UK |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915758/ http://www.ncbi.nlm.nih.gov/pubmed/31844107 https://doi.org/10.1038/s41598-019-55579-9 |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915758/ http://www.ncbi.nlm.nih.gov/pubmed/31844107 http://dx.doi.org/10.1038/s41598-019-55579-9 |
op_rights |
© The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-55579-9 |
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Scientific Reports |
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9 |
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