A methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost
One measure of hypoxia tolerance is the critical oxygen threshold, P(crit), which is the point where standard metabolism can no longer be maintained through aerobic processes. Traditionally, P(crit) was determined using closed respirometry, whereby the fish's respiration naturally lowered O(2)....
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ftpubmed:oai:pubmedcentral.nih.gov:6899028 2023-05-15T18:06:06+02:00 A methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost Negrete, Benjamin Esbaugh, Andrew J. 2019-11-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899028/ http://www.ncbi.nlm.nih.gov/pubmed/31649119 https://doi.org/10.1242/bio.045310 en eng The Company of Biologists Ltd http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899028/ http://www.ncbi.nlm.nih.gov/pubmed/31649119 http://dx.doi.org/10.1242/bio.045310 © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. CC-BY Methods and Techniques Text 2019 ftpubmed https://doi.org/10.1242/bio.045310 2019-12-15T01:44:08Z One measure of hypoxia tolerance is the critical oxygen threshold, P(crit), which is the point where standard metabolism can no longer be maintained through aerobic processes. Traditionally, P(crit) was determined using closed respirometry, whereby the fish's respiration naturally lowered O(2). More recently, intermittent flow techniques have been adopted, where N(2) is used to displace O(2), which ostensibly reduces end-product build-up. This study used a paired design on the marine teleost, red drum. P(crit) is comparable between closed (4.6±0.2 kPa; mean±s.e.m.) and intermittent flow (4.4±0.2 kPa; mean±s.e.m.) respirometry. pCO(2), ammonia and pH changes within the chamber were measured prior to the onset of P(crit) and at the end of a typical P(crit) trial and revealed changes in water chemistry in both closed and intermittent flow. P(crit) values were similar in both methods of hypoxia induction regardless of subsequent water chemistry changes that occurred in both methods. Text Red drum PubMed Central (PMC) Biology Open |
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Methods and Techniques |
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Methods and Techniques Negrete, Benjamin Esbaugh, Andrew J. A methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost |
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Methods and Techniques |
description |
One measure of hypoxia tolerance is the critical oxygen threshold, P(crit), which is the point where standard metabolism can no longer be maintained through aerobic processes. Traditionally, P(crit) was determined using closed respirometry, whereby the fish's respiration naturally lowered O(2). More recently, intermittent flow techniques have been adopted, where N(2) is used to displace O(2), which ostensibly reduces end-product build-up. This study used a paired design on the marine teleost, red drum. P(crit) is comparable between closed (4.6±0.2 kPa; mean±s.e.m.) and intermittent flow (4.4±0.2 kPa; mean±s.e.m.) respirometry. pCO(2), ammonia and pH changes within the chamber were measured prior to the onset of P(crit) and at the end of a typical P(crit) trial and revealed changes in water chemistry in both closed and intermittent flow. P(crit) values were similar in both methods of hypoxia induction regardless of subsequent water chemistry changes that occurred in both methods. |
format |
Text |
author |
Negrete, Benjamin Esbaugh, Andrew J. |
author_facet |
Negrete, Benjamin Esbaugh, Andrew J. |
author_sort |
Negrete, Benjamin |
title |
A methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost |
title_short |
A methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost |
title_full |
A methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost |
title_fullStr |
A methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost |
title_full_unstemmed |
A methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost |
title_sort |
methodological evaluation of the determination of critical oxygen threshold in an estuarine teleost |
publisher |
The Company of Biologists Ltd |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899028/ http://www.ncbi.nlm.nih.gov/pubmed/31649119 https://doi.org/10.1242/bio.045310 |
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Red drum |
genre_facet |
Red drum |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899028/ http://www.ncbi.nlm.nih.gov/pubmed/31649119 http://dx.doi.org/10.1242/bio.045310 |
op_rights |
© 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1242/bio.045310 |
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Biology Open |
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1766177685343567872 |