Processive recoding and metazoan evolution of Selenoprotein P: up to 132 UGAs in molluscs
Selenoproteins typically contain a single selenocysteine, the 21st amino acid, encoded by a context redefined UGA. However, human Selenoprotein P (SelenoP) has a redox-functioning selenocysteine in its N-terminal domain and 9 selenium transporter-functioning selenocysteines in its C-terminal domain....
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885538/ http://www.ncbi.nlm.nih.gov/pubmed/31442478 https://doi.org/10.1016/j.jmb.2019.08.007 |
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ftpubmed:oai:pubmedcentral.nih.gov:6885538 2023-05-15T17:54:20+02:00 Processive recoding and metazoan evolution of Selenoprotein P: up to 132 UGAs in molluscs Baclaocos, Janinah Santesmasses, Didac Mariotti, Marco Bierła, Katarzyna Vetick, Michael B. Lynch, Sharon McAllen, Rob Mackrill, John J. Loughran, Gary Guigó, Roderic Szpunar, Joanna Copeland, Paul R. Gladyshev, Vadim N Atkins, John F. 2019-08-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885538/ http://www.ncbi.nlm.nih.gov/pubmed/31442478 https://doi.org/10.1016/j.jmb.2019.08.007 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885538/ http://www.ncbi.nlm.nih.gov/pubmed/31442478 http://dx.doi.org/10.1016/j.jmb.2019.08.007 J Mol Biol Article Text 2019 ftpubmed https://doi.org/10.1016/j.jmb.2019.08.007 2020-11-15T01:16:51Z Selenoproteins typically contain a single selenocysteine, the 21st amino acid, encoded by a context redefined UGA. However, human Selenoprotein P (SelenoP) has a redox-functioning selenocysteine in its N-terminal domain and 9 selenium transporter-functioning selenocysteines in its C-terminal domain. Here we show that diverse SelenoP genes are present across metazoa with highly variable numbers of Sec-UGAs, ranging from a single UGA in certain insects, to 9 in common spider, and up to 132 in bivalve molluscs. SelenoP genes were shaped by a dynamic evolutionary process linked to selenium usage. Gene evolution featured modular expansions of an ancestral multi-Sec domain, which led to particularly Sec-rich SelenoP proteins in many aquatic organisms. We focused on molluscs, and chose Pacific oyster Magallana gigas as experimental model. We show that oyster SelenoP mRNA with 46 UGAs is translated full-length in vivo. Ribosome profiling indicates that selenocysteine specification occurs with ~5% efficiency at UGA1 and approaches 100% efficiency at distal 3’ UGAs. We report genetic elements relevant to its expression, including a leader ORF and an RNA structure overlapping the initiation codon that modulates ribosome progression in a selenium dependent manner. Unlike their mammalian counterparts, the two SECIS elements in oyster SelenoP (3’UTR recoding elements) do not show functional differentiation in vitro. Oysters can increase their tissue selenium level up to 50-fold upon supplementation, which also results in extensive changes in selenoprotein expression. Text Pacific oyster PubMed Central (PMC) Pacific Journal of Molecular Biology 431 22 4381 4407 |
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Article Baclaocos, Janinah Santesmasses, Didac Mariotti, Marco Bierła, Katarzyna Vetick, Michael B. Lynch, Sharon McAllen, Rob Mackrill, John J. Loughran, Gary Guigó, Roderic Szpunar, Joanna Copeland, Paul R. Gladyshev, Vadim N Atkins, John F. Processive recoding and metazoan evolution of Selenoprotein P: up to 132 UGAs in molluscs |
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Article |
description |
Selenoproteins typically contain a single selenocysteine, the 21st amino acid, encoded by a context redefined UGA. However, human Selenoprotein P (SelenoP) has a redox-functioning selenocysteine in its N-terminal domain and 9 selenium transporter-functioning selenocysteines in its C-terminal domain. Here we show that diverse SelenoP genes are present across metazoa with highly variable numbers of Sec-UGAs, ranging from a single UGA in certain insects, to 9 in common spider, and up to 132 in bivalve molluscs. SelenoP genes were shaped by a dynamic evolutionary process linked to selenium usage. Gene evolution featured modular expansions of an ancestral multi-Sec domain, which led to particularly Sec-rich SelenoP proteins in many aquatic organisms. We focused on molluscs, and chose Pacific oyster Magallana gigas as experimental model. We show that oyster SelenoP mRNA with 46 UGAs is translated full-length in vivo. Ribosome profiling indicates that selenocysteine specification occurs with ~5% efficiency at UGA1 and approaches 100% efficiency at distal 3’ UGAs. We report genetic elements relevant to its expression, including a leader ORF and an RNA structure overlapping the initiation codon that modulates ribosome progression in a selenium dependent manner. Unlike their mammalian counterparts, the two SECIS elements in oyster SelenoP (3’UTR recoding elements) do not show functional differentiation in vitro. Oysters can increase their tissue selenium level up to 50-fold upon supplementation, which also results in extensive changes in selenoprotein expression. |
format |
Text |
author |
Baclaocos, Janinah Santesmasses, Didac Mariotti, Marco Bierła, Katarzyna Vetick, Michael B. Lynch, Sharon McAllen, Rob Mackrill, John J. Loughran, Gary Guigó, Roderic Szpunar, Joanna Copeland, Paul R. Gladyshev, Vadim N Atkins, John F. |
author_facet |
Baclaocos, Janinah Santesmasses, Didac Mariotti, Marco Bierła, Katarzyna Vetick, Michael B. Lynch, Sharon McAllen, Rob Mackrill, John J. Loughran, Gary Guigó, Roderic Szpunar, Joanna Copeland, Paul R. Gladyshev, Vadim N Atkins, John F. |
author_sort |
Baclaocos, Janinah |
title |
Processive recoding and metazoan evolution of Selenoprotein P: up to 132 UGAs in molluscs |
title_short |
Processive recoding and metazoan evolution of Selenoprotein P: up to 132 UGAs in molluscs |
title_full |
Processive recoding and metazoan evolution of Selenoprotein P: up to 132 UGAs in molluscs |
title_fullStr |
Processive recoding and metazoan evolution of Selenoprotein P: up to 132 UGAs in molluscs |
title_full_unstemmed |
Processive recoding and metazoan evolution of Selenoprotein P: up to 132 UGAs in molluscs |
title_sort |
processive recoding and metazoan evolution of selenoprotein p: up to 132 ugas in molluscs |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885538/ http://www.ncbi.nlm.nih.gov/pubmed/31442478 https://doi.org/10.1016/j.jmb.2019.08.007 |
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Pacific |
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Pacific |
genre |
Pacific oyster |
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Pacific oyster |
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J Mol Biol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885538/ http://www.ncbi.nlm.nih.gov/pubmed/31442478 http://dx.doi.org/10.1016/j.jmb.2019.08.007 |
op_doi |
https://doi.org/10.1016/j.jmb.2019.08.007 |
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Journal of Molecular Biology |
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431 |
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22 |
container_start_page |
4381 |
op_container_end_page |
4407 |
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1766162077235281920 |