Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The s...
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ftpubmed:oai:pubmedcentral.nih.gov:6864645 2023-05-15T15:38:52+02:00 Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Schneider, Yannik K.-H. Ø. Hansen, Kine Isaksson, Johan Ullsten, Sara H. Hansen, Espen Hammer Andersen, Jeanette 2019-11-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864645/ http://www.ncbi.nlm.nih.gov/pubmed/31694159 https://doi.org/10.3390/molecules24213991 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864645/ http://www.ncbi.nlm.nih.gov/pubmed/31694159 http://dx.doi.org/10.3390/molecules24213991 © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2019 ftpubmed https://doi.org/10.3390/molecules24213991 2019-12-29T01:13:55Z Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. Text Barents Sea PubMed Central (PMC) Barents Sea Molecules 24 21 3991 |
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Article Schneider, Yannik K.-H. Ø. Hansen, Kine Isaksson, Johan Ullsten, Sara H. Hansen, Espen Hammer Andersen, Jeanette Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
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Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter, were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. |
format |
Text |
author |
Schneider, Yannik K.-H. Ø. Hansen, Kine Isaksson, Johan Ullsten, Sara H. Hansen, Espen Hammer Andersen, Jeanette |
author_facet |
Schneider, Yannik K.-H. Ø. Hansen, Kine Isaksson, Johan Ullsten, Sara H. Hansen, Espen Hammer Andersen, Jeanette |
author_sort |
Schneider, Yannik K.-H. |
title |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_short |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_full |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_fullStr |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_full_unstemmed |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_sort |
anti-bacterial effect and cytotoxicity assessment of lipid 430 isolated from algibacter sp. |
publisher |
MDPI |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864645/ http://www.ncbi.nlm.nih.gov/pubmed/31694159 https://doi.org/10.3390/molecules24213991 |
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Barents Sea |
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Barents Sea |
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Barents Sea |
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Barents Sea |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864645/ http://www.ncbi.nlm.nih.gov/pubmed/31694159 http://dx.doi.org/10.3390/molecules24213991 |
op_rights |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
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https://doi.org/10.3390/molecules24213991 |
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Molecules |
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