Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae
Ocean acidification (OA), a direct consequence of increasing atmospheric CO(2) concentration dissolving in ocean waters, is impacting many fish species. Little is known about the molecular mechanisms underlying the observed physiological impacts in fish. We used RNAseq to characterize the transcript...
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ftpubmed:oai:pubmedcentral.nih.gov:6858462 2023-05-15T15:26:59+02:00 Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae Mittermayer, F. H. Stiasny, M. H. Clemmesen, C. Bayer, T. Puvanendran, V. Chierici, M. Jentoft, S. Reusch, T. B. H. 2019-11-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858462/ http://www.ncbi.nlm.nih.gov/pubmed/31729401 https://doi.org/10.1038/s41598-019-52628-1 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858462/ http://www.ncbi.nlm.nih.gov/pubmed/31729401 http://dx.doi.org/10.1038/s41598-019-52628-1 © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2019 ftpubmed https://doi.org/10.1038/s41598-019-52628-1 2019-12-01T01:24:20Z Ocean acidification (OA), a direct consequence of increasing atmospheric CO(2) concentration dissolving in ocean waters, is impacting many fish species. Little is known about the molecular mechanisms underlying the observed physiological impacts in fish. We used RNAseq to characterize the transcriptome of 3 different larval stages of Atlantic cod (Gadus morhua) exposed to simulated OA at levels (1179 µatm CO(2)) representing end-of-century predictions compared to controls (503 µatm CO(2)), which were shown to induce tissue damage and elevated mortality in G. morhua. Only few genes were differentially expressed in 6 and 13 days-post-hatching (dph) (3 and 16 genes, respectively), during a period when maximal mortality as a response to elevated pCO(2) occurred. At 36 dph, 1413 genes were differentially expressed, most likely caused by developmental asynchrony between the treatment groups, with individuals under OA growing faster. A target gene analysis revealed only few genes of the universal and well-defined cellular stress response to be differentially expressed. We thus suggest that predicted ocean acidification levels constitute a “stealth stress” for early Atlantic cod larvae, with a rapid breakdown of cellular homeostasis leading to organismal death that was missed even with an 8-fold replication implemented in this study. Text atlantic cod Gadus morhua Ocean acidification PubMed Central (PMC) Scientific Reports 9 1 |
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Article Mittermayer, F. H. Stiasny, M. H. Clemmesen, C. Bayer, T. Puvanendran, V. Chierici, M. Jentoft, S. Reusch, T. B. H. Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae |
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Ocean acidification (OA), a direct consequence of increasing atmospheric CO(2) concentration dissolving in ocean waters, is impacting many fish species. Little is known about the molecular mechanisms underlying the observed physiological impacts in fish. We used RNAseq to characterize the transcriptome of 3 different larval stages of Atlantic cod (Gadus morhua) exposed to simulated OA at levels (1179 µatm CO(2)) representing end-of-century predictions compared to controls (503 µatm CO(2)), which were shown to induce tissue damage and elevated mortality in G. morhua. Only few genes were differentially expressed in 6 and 13 days-post-hatching (dph) (3 and 16 genes, respectively), during a period when maximal mortality as a response to elevated pCO(2) occurred. At 36 dph, 1413 genes were differentially expressed, most likely caused by developmental asynchrony between the treatment groups, with individuals under OA growing faster. A target gene analysis revealed only few genes of the universal and well-defined cellular stress response to be differentially expressed. We thus suggest that predicted ocean acidification levels constitute a “stealth stress” for early Atlantic cod larvae, with a rapid breakdown of cellular homeostasis leading to organismal death that was missed even with an 8-fold replication implemented in this study. |
format |
Text |
author |
Mittermayer, F. H. Stiasny, M. H. Clemmesen, C. Bayer, T. Puvanendran, V. Chierici, M. Jentoft, S. Reusch, T. B. H. |
author_facet |
Mittermayer, F. H. Stiasny, M. H. Clemmesen, C. Bayer, T. Puvanendran, V. Chierici, M. Jentoft, S. Reusch, T. B. H. |
author_sort |
Mittermayer, F. H. |
title |
Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae |
title_short |
Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae |
title_full |
Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae |
title_fullStr |
Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae |
title_full_unstemmed |
Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae |
title_sort |
transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for atlantic cod larvae |
publisher |
Nature Publishing Group UK |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858462/ http://www.ncbi.nlm.nih.gov/pubmed/31729401 https://doi.org/10.1038/s41598-019-52628-1 |
genre |
atlantic cod Gadus morhua Ocean acidification |
genre_facet |
atlantic cod Gadus morhua Ocean acidification |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6858462/ http://www.ncbi.nlm.nih.gov/pubmed/31729401 http://dx.doi.org/10.1038/s41598-019-52628-1 |
op_rights |
© The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-52628-1 |
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Scientific Reports |
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