Effects of Sanionia uncinata extracts in protecting against and inducing DNA cleavage by reactive oxygen species
When mosses are exposed to increased quantities of ultraviolet (UV) radiation, they produce more secondary metabolites. Antarctica moss Sanionia uncinata (Hedw.) Loeske has presented high carotenoid contents in response to an increase in UVB radiation. This moss has been recommended as a potential s...
Published in: | Redox Report |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
Taylor & Francis
2013
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837662/ http://www.ncbi.nlm.nih.gov/pubmed/22005340 https://doi.org/10.1179/1351000211Y.0000000011 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:6837662 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:6837662 2023-05-15T13:47:07+02:00 Effects of Sanionia uncinata extracts in protecting against and inducing DNA cleavage by reactive oxygen species Fernandes, Andréia da Silva Mazzei, José Luiz de Alencar, Alexandre Santos Evangelista, Heitor Felzenszwalb, Israel 2013-07-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837662/ http://www.ncbi.nlm.nih.gov/pubmed/22005340 https://doi.org/10.1179/1351000211Y.0000000011 en eng Taylor & Francis http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837662/ http://www.ncbi.nlm.nih.gov/pubmed/22005340 http://dx.doi.org/10.1179/1351000211Y.0000000011 © W.S. Maney & Son Ltd 2011 Research Article Text 2013 ftpubmed https://doi.org/10.1179/1351000211Y.0000000011 2020-04-19T00:17:11Z When mosses are exposed to increased quantities of ultraviolet (UV) radiation, they produce more secondary metabolites. Antarctica moss Sanionia uncinata (Hedw.) Loeske has presented high carotenoid contents in response to an increase in UVB radiation. This moss has been recommended as a potential source of antioxidants. In the present work, the protective and enhancing effects of aqueous (AE) and hydroalcoholic (HE) extracts of S. uncinata on the cleavage of supercoiled DNA were evaluated through topological modifications, quantified by densitometry after agarose gel electrophoresis. Total phenolic contents reached 5.89 mg/g. Our data demonstrated that the extract does not induce DNA cleavage. Furthermore, both extracts showed antioxidant activity that protected the DNA against cleavage induced by (i) O(2)(•−), 89% (AE) and 94% (HE) (P < 0.05), and (ii) .OH, 17% (AE) and 18% (HE). However, the extracts intensified cleavage induced by Fenton-like reactions: (i) Cu(2+)/H(2)O(2), 94% (AE) and 100% (HE) (P < 0.05), and (ii) SnCl(2), 62% (AE) and 56% (HE). DNA damages seem to follow different ways: (i) in the presence of Fenton-like reactions could be via reactive oxygen species generation and (ii) with HE/Cu(2+) could have also been triggered by other mechanisms. Text Antarc* Antarctica PubMed Central (PMC) Fenton ENVELOPE(161.917,161.917,-74.333,-74.333) Redox Report 16 5 201 207 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Fernandes, Andréia da Silva Mazzei, José Luiz de Alencar, Alexandre Santos Evangelista, Heitor Felzenszwalb, Israel Effects of Sanionia uncinata extracts in protecting against and inducing DNA cleavage by reactive oxygen species |
topic_facet |
Research Article |
description |
When mosses are exposed to increased quantities of ultraviolet (UV) radiation, they produce more secondary metabolites. Antarctica moss Sanionia uncinata (Hedw.) Loeske has presented high carotenoid contents in response to an increase in UVB radiation. This moss has been recommended as a potential source of antioxidants. In the present work, the protective and enhancing effects of aqueous (AE) and hydroalcoholic (HE) extracts of S. uncinata on the cleavage of supercoiled DNA were evaluated through topological modifications, quantified by densitometry after agarose gel electrophoresis. Total phenolic contents reached 5.89 mg/g. Our data demonstrated that the extract does not induce DNA cleavage. Furthermore, both extracts showed antioxidant activity that protected the DNA against cleavage induced by (i) O(2)(•−), 89% (AE) and 94% (HE) (P < 0.05), and (ii) .OH, 17% (AE) and 18% (HE). However, the extracts intensified cleavage induced by Fenton-like reactions: (i) Cu(2+)/H(2)O(2), 94% (AE) and 100% (HE) (P < 0.05), and (ii) SnCl(2), 62% (AE) and 56% (HE). DNA damages seem to follow different ways: (i) in the presence of Fenton-like reactions could be via reactive oxygen species generation and (ii) with HE/Cu(2+) could have also been triggered by other mechanisms. |
format |
Text |
author |
Fernandes, Andréia da Silva Mazzei, José Luiz de Alencar, Alexandre Santos Evangelista, Heitor Felzenszwalb, Israel |
author_facet |
Fernandes, Andréia da Silva Mazzei, José Luiz de Alencar, Alexandre Santos Evangelista, Heitor Felzenszwalb, Israel |
author_sort |
Fernandes, Andréia da Silva |
title |
Effects of Sanionia uncinata extracts in protecting against and inducing DNA cleavage by reactive oxygen species |
title_short |
Effects of Sanionia uncinata extracts in protecting against and inducing DNA cleavage by reactive oxygen species |
title_full |
Effects of Sanionia uncinata extracts in protecting against and inducing DNA cleavage by reactive oxygen species |
title_fullStr |
Effects of Sanionia uncinata extracts in protecting against and inducing DNA cleavage by reactive oxygen species |
title_full_unstemmed |
Effects of Sanionia uncinata extracts in protecting against and inducing DNA cleavage by reactive oxygen species |
title_sort |
effects of sanionia uncinata extracts in protecting against and inducing dna cleavage by reactive oxygen species |
publisher |
Taylor & Francis |
publishDate |
2013 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837662/ http://www.ncbi.nlm.nih.gov/pubmed/22005340 https://doi.org/10.1179/1351000211Y.0000000011 |
long_lat |
ENVELOPE(161.917,161.917,-74.333,-74.333) |
geographic |
Fenton |
geographic_facet |
Fenton |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837662/ http://www.ncbi.nlm.nih.gov/pubmed/22005340 http://dx.doi.org/10.1179/1351000211Y.0000000011 |
op_rights |
© W.S. Maney & Son Ltd 2011 |
op_doi |
https://doi.org/10.1179/1351000211Y.0000000011 |
container_title |
Redox Report |
container_volume |
16 |
container_issue |
5 |
container_start_page |
201 |
op_container_end_page |
207 |
_version_ |
1766246368305741824 |