Amelioration of anti-hepatotoxic effect by Lichen rangiferinus against alcohol induced liver damage in rats
BACKGROUND: Reindeer lichen, Lichen rangiferinus syn. or Cladonia rangiferina (L.) F. H. Wigg. (Cladoniaceae) has been traditionally reported as a remedy to treat fever, colds, arthritis as well as convulsions, liver infections, coughs, constipation, and tuberculosis. The current study is aimed at r...
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ftpubmed:oai:pubmedcentral.nih.gov:6822147 2023-05-15T15:55:39+02:00 Amelioration of anti-hepatotoxic effect by Lichen rangiferinus against alcohol induced liver damage in rats Shukla, Ila Azmi, Lubna Gupta, Shyam Sundar Upreti, Dalip Kumar Rao, Chandana Venkateswara 2019 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822147/ http://www.ncbi.nlm.nih.gov/pubmed/29395895 https://doi.org/10.1016/j.jaim.2017.08.007 en eng Elsevier http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822147/ http://www.ncbi.nlm.nih.gov/pubmed/29395895 http://dx.doi.org/10.1016/j.jaim.2017.08.007 © 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). CC-BY-NC-ND Original Research Articles- Experimental Text 2019 ftpubmed https://doi.org/10.1016/j.jaim.2017.08.007 2019-11-10T01:27:17Z BACKGROUND: Reindeer lichen, Lichen rangiferinus syn. or Cladonia rangiferina (L.) F. H. Wigg. (Cladoniaceae) has been traditionally reported as a remedy to treat fever, colds, arthritis as well as convulsions, liver infections, coughs, constipation, and tuberculosis. The current study is aimed at rectification of alcohol induced liver damage by the use of L. rangiferinus extract. OBJECTIVES: The aim of the study was to compare some biochemical markers for liver injury and hematological indices in normal untreated rats and treated rats. MATERIAL AND METHODS: The study was performed using male Wistar rats. Animals were categorized into five groups, negative control group (normal diet only), treated groups (2 groups were lichen treated along with 10% ethanol & 1 group was only ethanol treated) and positive control group (Silymarin + 10% ethanol) of six animals in each group. Biochemical markers for liver injury and hematological indices of all animals were measured using standard diagnostic tools. The animals were then sacrificed and livers were sent to the pathology lab for histopathological analysis. RESULTS: Lichen extract showed a significant restoration of altered biochemical parameters towards normal in both in vitro and in vivo conditions. The total phenolic and flavonoid content of the LRE was found to be 21.78 μg PE/mg of extract and 5.13 μg RE/mg of extract respectively. The IC(50) values for atranorin and fumarprotocetraric acid were found to be 128.48 and 218.46 mg/mL respectively. Reducing power of the extract was found to be quite significant. After administration of lichen extract, endothelial cells were less injured around central vein and number of fat vacuoles was also lesser in hepatocytes. CONCLUSION: Conclusively, treatment with lichen extract assuages alcohol-related damage and guards hepatic tissue from alcohol-induced toxicity. Text Cladonia rangiferina Reindeer lichen PubMed Central (PMC) Journal of Ayurveda and Integrative Medicine 10 3 171 177 |
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Original Research Articles- Experimental Shukla, Ila Azmi, Lubna Gupta, Shyam Sundar Upreti, Dalip Kumar Rao, Chandana Venkateswara Amelioration of anti-hepatotoxic effect by Lichen rangiferinus against alcohol induced liver damage in rats |
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Original Research Articles- Experimental |
description |
BACKGROUND: Reindeer lichen, Lichen rangiferinus syn. or Cladonia rangiferina (L.) F. H. Wigg. (Cladoniaceae) has been traditionally reported as a remedy to treat fever, colds, arthritis as well as convulsions, liver infections, coughs, constipation, and tuberculosis. The current study is aimed at rectification of alcohol induced liver damage by the use of L. rangiferinus extract. OBJECTIVES: The aim of the study was to compare some biochemical markers for liver injury and hematological indices in normal untreated rats and treated rats. MATERIAL AND METHODS: The study was performed using male Wistar rats. Animals were categorized into five groups, negative control group (normal diet only), treated groups (2 groups were lichen treated along with 10% ethanol & 1 group was only ethanol treated) and positive control group (Silymarin + 10% ethanol) of six animals in each group. Biochemical markers for liver injury and hematological indices of all animals were measured using standard diagnostic tools. The animals were then sacrificed and livers were sent to the pathology lab for histopathological analysis. RESULTS: Lichen extract showed a significant restoration of altered biochemical parameters towards normal in both in vitro and in vivo conditions. The total phenolic and flavonoid content of the LRE was found to be 21.78 μg PE/mg of extract and 5.13 μg RE/mg of extract respectively. The IC(50) values for atranorin and fumarprotocetraric acid were found to be 128.48 and 218.46 mg/mL respectively. Reducing power of the extract was found to be quite significant. After administration of lichen extract, endothelial cells were less injured around central vein and number of fat vacuoles was also lesser in hepatocytes. CONCLUSION: Conclusively, treatment with lichen extract assuages alcohol-related damage and guards hepatic tissue from alcohol-induced toxicity. |
format |
Text |
author |
Shukla, Ila Azmi, Lubna Gupta, Shyam Sundar Upreti, Dalip Kumar Rao, Chandana Venkateswara |
author_facet |
Shukla, Ila Azmi, Lubna Gupta, Shyam Sundar Upreti, Dalip Kumar Rao, Chandana Venkateswara |
author_sort |
Shukla, Ila |
title |
Amelioration of anti-hepatotoxic effect by Lichen rangiferinus against alcohol induced liver damage in rats |
title_short |
Amelioration of anti-hepatotoxic effect by Lichen rangiferinus against alcohol induced liver damage in rats |
title_full |
Amelioration of anti-hepatotoxic effect by Lichen rangiferinus against alcohol induced liver damage in rats |
title_fullStr |
Amelioration of anti-hepatotoxic effect by Lichen rangiferinus against alcohol induced liver damage in rats |
title_full_unstemmed |
Amelioration of anti-hepatotoxic effect by Lichen rangiferinus against alcohol induced liver damage in rats |
title_sort |
amelioration of anti-hepatotoxic effect by lichen rangiferinus against alcohol induced liver damage in rats |
publisher |
Elsevier |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822147/ http://www.ncbi.nlm.nih.gov/pubmed/29395895 https://doi.org/10.1016/j.jaim.2017.08.007 |
genre |
Cladonia rangiferina Reindeer lichen |
genre_facet |
Cladonia rangiferina Reindeer lichen |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822147/ http://www.ncbi.nlm.nih.gov/pubmed/29395895 http://dx.doi.org/10.1016/j.jaim.2017.08.007 |
op_rights |
© 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.jaim.2017.08.007 |
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Journal of Ayurveda and Integrative Medicine |
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10 |
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3 |
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171 |
op_container_end_page |
177 |
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