Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.)
The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 10(6) CFU/g). In this context,...
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ftpubmed:oai:pubmedcentral.nih.gov:6777325 2023-05-15T15:31:15+02:00 Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) Jaramillo-Torres, Alexander Rawling, Mark D. Rodiles, Ana Mikalsen, Heidi E. Johansen, Lill-Heidi Tinsley, John Forberg, Torunn Aasum, Elisabeth Castex, Mathieu Merrifield, Daniel Lee 2019-09-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777325/ http://www.ncbi.nlm.nih.gov/pubmed/31611864 https://doi.org/10.3389/fmicb.2019.02243 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777325/ http://www.ncbi.nlm.nih.gov/pubmed/31611864 http://dx.doi.org/10.3389/fmicb.2019.02243 Copyright © 2019 Jaramillo-Torres, Rawling, Rodiles, Mikalsen, Johansen, Tinsley, Forberg, Aasum, Castex and Merrifield. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2019 ftpubmed https://doi.org/10.3389/fmicb.2019.02243 2019-10-20T00:19:27Z The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 10(6) CFU/g). In this context, fish health and antiviral response were also investigated. A 12-week feeding trial was conducted in a flow-through rearing system involving 6 weeks in freshwater and 6 weeks in seawater. Fish received a control and probiotic diet. The composition of the salmon gut bacterial communities was determined by high-throughput sequencing of digesta and mucosa samples from both the freshwater and seawater stage. The main phyla detected during both freshwater and seawater stages were Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. Significant differences were observed between the intestinal microbiota in the digesta and the mucosa. Both probiotic supplementation and the seawater transfer (SWT) had a substantial impact on the microbial communities, with most pronounced changes detected in the mucosal communities after SWT. This last finding together with a significantly higher antiviral response (mx-1 and tlr3 gene expression) in the distal intestine of fish fed the probiotic diet suggest a causal link between the microbiota modulation and activation of antiviral response. Feeding probiotics during the freshwater stage did not significantly increase survival after infectious pancreatic necrosis virus (IPNV) challenge after SWT, although higher survival was observed in one out of two replicate challenge tanks. In conclusion, this study demonstrated that both dietary probiotic supplementation and transfer from freshwater to seawater have an important role in modulating the bacterial communities in the distal intestine of Atlantic salmon. Furthermore, supplementation of the diet with P. acidilactici MA18/5M can modulate antiviral response. Text Atlantic salmon Salmo salar PubMed Central (PMC) Frontiers in Microbiology 10 |
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English |
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Microbiology |
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Microbiology Jaramillo-Torres, Alexander Rawling, Mark D. Rodiles, Ana Mikalsen, Heidi E. Johansen, Lill-Heidi Tinsley, John Forberg, Torunn Aasum, Elisabeth Castex, Mathieu Merrifield, Daniel Lee Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
topic_facet |
Microbiology |
description |
The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 10(6) CFU/g). In this context, fish health and antiviral response were also investigated. A 12-week feeding trial was conducted in a flow-through rearing system involving 6 weeks in freshwater and 6 weeks in seawater. Fish received a control and probiotic diet. The composition of the salmon gut bacterial communities was determined by high-throughput sequencing of digesta and mucosa samples from both the freshwater and seawater stage. The main phyla detected during both freshwater and seawater stages were Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. Significant differences were observed between the intestinal microbiota in the digesta and the mucosa. Both probiotic supplementation and the seawater transfer (SWT) had a substantial impact on the microbial communities, with most pronounced changes detected in the mucosal communities after SWT. This last finding together with a significantly higher antiviral response (mx-1 and tlr3 gene expression) in the distal intestine of fish fed the probiotic diet suggest a causal link between the microbiota modulation and activation of antiviral response. Feeding probiotics during the freshwater stage did not significantly increase survival after infectious pancreatic necrosis virus (IPNV) challenge after SWT, although higher survival was observed in one out of two replicate challenge tanks. In conclusion, this study demonstrated that both dietary probiotic supplementation and transfer from freshwater to seawater have an important role in modulating the bacterial communities in the distal intestine of Atlantic salmon. Furthermore, supplementation of the diet with P. acidilactici MA18/5M can modulate antiviral response. |
format |
Text |
author |
Jaramillo-Torres, Alexander Rawling, Mark D. Rodiles, Ana Mikalsen, Heidi E. Johansen, Lill-Heidi Tinsley, John Forberg, Torunn Aasum, Elisabeth Castex, Mathieu Merrifield, Daniel Lee |
author_facet |
Jaramillo-Torres, Alexander Rawling, Mark D. Rodiles, Ana Mikalsen, Heidi E. Johansen, Lill-Heidi Tinsley, John Forberg, Torunn Aasum, Elisabeth Castex, Mathieu Merrifield, Daniel Lee |
author_sort |
Jaramillo-Torres, Alexander |
title |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_short |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_full |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_fullStr |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_full_unstemmed |
Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.) |
title_sort |
influence of dietary supplementation of probiotic pediococcus acidilactici ma18/5m during the transition from freshwater to seawater on intestinal health and microbiota of atlantic salmon (salmo salar l.) |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777325/ http://www.ncbi.nlm.nih.gov/pubmed/31611864 https://doi.org/10.3389/fmicb.2019.02243 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777325/ http://www.ncbi.nlm.nih.gov/pubmed/31611864 http://dx.doi.org/10.3389/fmicb.2019.02243 |
op_rights |
Copyright © 2019 Jaramillo-Torres, Rawling, Rodiles, Mikalsen, Johansen, Tinsley, Forberg, Aasum, Castex and Merrifield. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2019.02243 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766361738951786496 |