Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor)
Marine mammals, such as whales, have a high proportion of body fat and so are susceptible to the accumulation, and associated detrimental health effects, of lipophilic environmental contaminants. Recently, we developed a wild-type cell line from humpback whale fibroblasts (HuWa). Extensive molecular...
Published in: | Cell Biology and Toxicology |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
Springer Netherlands
2019
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757015/ http://www.ncbi.nlm.nih.gov/pubmed/30627956 https://doi.org/10.1007/s10565-018-09457-1 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:6757015 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:6757015 2023-05-15T16:35:54+02:00 Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor) Burkard, Michael Bengtson Nash, Susan Gambaro, Gessica Whitworth, Deanne Schirmer, Kristin 2019-01-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757015/ http://www.ncbi.nlm.nih.gov/pubmed/30627956 https://doi.org/10.1007/s10565-018-09457-1 en eng Springer Netherlands http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757015/ http://www.ncbi.nlm.nih.gov/pubmed/30627956 http://dx.doi.org/10.1007/s10565-018-09457-1 © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. CC-BY Original Article Text 2019 ftpubmed https://doi.org/10.1007/s10565-018-09457-1 2019-10-13T00:13:59Z Marine mammals, such as whales, have a high proportion of body fat and so are susceptible to the accumulation, and associated detrimental health effects, of lipophilic environmental contaminants. Recently, we developed a wild-type cell line from humpback whale fibroblasts (HuWa). Extensive molecular assessments with mammalian wild-type cells are typically constrained by a finite life span, with cells eventually becoming senescent. Thus, the present work explored the possibility of preventing senescence in the HuWa cell line by transfection with plasmids encoding the simian virus large T antigen (SV40T) or telomerase reverse transcriptase (TERT). No stable expression was achieved upon SV40 transfection. Transfection with TERT, on the other hand, activated the expression of telomerase in HuWa cells. At the time of manuscript preparation, the transfected HuWa cells (HuWa(TERT)) have been stable for at least 59 passages post-transfection. HuWa(TERT) proliferate rapidly and maintain initial cell characteristics, such as morphology and chromosomal stability. The response of HuWa(TERT) cells to an immune stimulant (lipopolysaccharide (LPS)) and an immunotoxicant (Aroclor1254) was assessed by measurement of intracellular levels of the pro-inflammatory cytokines interleukin (IL)-6, IL-1β and tumour necrosis factor (TNF)-α. HuWa(TERT) cells constitutively express IL-6, IL-1β and TNFα. Exposure to neither LPS nor Aroclor1254 had an effect on the levels of these cytokines. Overall, this work supports the diverse applicability of HuWa cell lines in that they display reliable long-term preservation, susceptibility to exogenous gene transfer and enable the study of humpback whale-specific cellular response mechanisms. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10565-018-09457-1) contains supplementary material, which is available to authorized users. Text Humpback Whale PubMed Central (PMC) Cell Biology and Toxicology 35 4 387 398 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Original Article |
spellingShingle |
Original Article Burkard, Michael Bengtson Nash, Susan Gambaro, Gessica Whitworth, Deanne Schirmer, Kristin Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor) |
topic_facet |
Original Article |
description |
Marine mammals, such as whales, have a high proportion of body fat and so are susceptible to the accumulation, and associated detrimental health effects, of lipophilic environmental contaminants. Recently, we developed a wild-type cell line from humpback whale fibroblasts (HuWa). Extensive molecular assessments with mammalian wild-type cells are typically constrained by a finite life span, with cells eventually becoming senescent. Thus, the present work explored the possibility of preventing senescence in the HuWa cell line by transfection with plasmids encoding the simian virus large T antigen (SV40T) or telomerase reverse transcriptase (TERT). No stable expression was achieved upon SV40 transfection. Transfection with TERT, on the other hand, activated the expression of telomerase in HuWa cells. At the time of manuscript preparation, the transfected HuWa cells (HuWa(TERT)) have been stable for at least 59 passages post-transfection. HuWa(TERT) proliferate rapidly and maintain initial cell characteristics, such as morphology and chromosomal stability. The response of HuWa(TERT) cells to an immune stimulant (lipopolysaccharide (LPS)) and an immunotoxicant (Aroclor1254) was assessed by measurement of intracellular levels of the pro-inflammatory cytokines interleukin (IL)-6, IL-1β and tumour necrosis factor (TNF)-α. HuWa(TERT) cells constitutively express IL-6, IL-1β and TNFα. Exposure to neither LPS nor Aroclor1254 had an effect on the levels of these cytokines. Overall, this work supports the diverse applicability of HuWa cell lines in that they display reliable long-term preservation, susceptibility to exogenous gene transfer and enable the study of humpback whale-specific cellular response mechanisms. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10565-018-09457-1) contains supplementary material, which is available to authorized users. |
format |
Text |
author |
Burkard, Michael Bengtson Nash, Susan Gambaro, Gessica Whitworth, Deanne Schirmer, Kristin |
author_facet |
Burkard, Michael Bengtson Nash, Susan Gambaro, Gessica Whitworth, Deanne Schirmer, Kristin |
author_sort |
Burkard, Michael |
title |
Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor) |
title_short |
Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor) |
title_full |
Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor) |
title_fullStr |
Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor) |
title_full_unstemmed |
Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor) |
title_sort |
lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (lps) and a mixture of polychlorinated biphenyls (aroclor) |
publisher |
Springer Netherlands |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757015/ http://www.ncbi.nlm.nih.gov/pubmed/30627956 https://doi.org/10.1007/s10565-018-09457-1 |
genre |
Humpback Whale |
genre_facet |
Humpback Whale |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757015/ http://www.ncbi.nlm.nih.gov/pubmed/30627956 http://dx.doi.org/10.1007/s10565-018-09457-1 |
op_rights |
© The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s10565-018-09457-1 |
container_title |
Cell Biology and Toxicology |
container_volume |
35 |
container_issue |
4 |
container_start_page |
387 |
op_container_end_page |
398 |
_version_ |
1766026207528222720 |