(13)C values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish
BACKGROUND: Stable isotope analysis of single amino acids (AA) is usually applied in food web studies for tracing biosynthetic origins of AA carbon backbones and establishing trophic positions of consumers, but the method is also showing promise for characterizing quantity and quality of dietary lip...
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ftpubmed:oai:pubmedcentral.nih.gov:6754727 2023-05-15T15:32:47+02:00 (13)C values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish Wang, Yiming V. Wan, Alex H.L. Krogdahl, Åshild Johnson, Mark Larsen, Thomas 2019-09-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754727/ https://doi.org/10.7717/peerj.7701 en eng PeerJ Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754727/ http://dx.doi.org/10.7717/peerj.7701 ©2019 Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. CC-BY Aquaculture Fisheries and Fish Science Text 2019 ftpubmed https://doi.org/10.7717/peerj.7701 2019-10-06T00:32:42Z BACKGROUND: Stable isotope analysis of single amino acids (AA) is usually applied in food web studies for tracing biosynthetic origins of AA carbon backbones and establishing trophic positions of consumers, but the method is also showing promise for characterizing quantity and quality of dietary lipids and carbohydrates. METHODS: To investigate whether changes in high- and low-digestible carbohydrates affect δ(13)C values of glycolytic AA, i.e., AA carbon backbones sourced from the glycolytic pathway, we compared Atlantic salmon (Salmo salar) from a feeding experiment with and without dietary inclusion of the red macroalga Palmaria palmata. The Control and experimental diets had similar relative proportions of macronutrients, but their ingredients differed; in the experimental treatment, 15% Palmaria inclusion substituted proteins from fishmeal and carbohydrates from corn starch. RESULTS: We found that (13)C values of the glycolytic AA were highly sensitive to substitution of corn starch with Palmaria. The δ(13)C offsets of glycolytic AA between salmon and their diets were significantly greater in the Palmaria inclusion than Control treatment. This greater offset can be attributed to the different utilization of high- vs. low-digestible carbohydrate sources, i.e., corn starch vs. Palmaria, in the two treatments, and metabolic routing of dietary lipids. In addition, similar δ(13)C values of essential AA between treatments indicate similar nutrient assimilation efficiency for all terrestrial (pea protein concentrate and wheat gluten meal) and marine (fishmeal and red alga) derived protein sources. These results show that δ(13)C(AA) analysis is a promising tool for improving our understanding of how carnivorous fish utilize macronutrient and route metabolic intermediates to tissue. Text Atlantic salmon Salmo salar PubMed Central (PMC) PeerJ 7 e7701 |
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English |
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Aquaculture Fisheries and Fish Science |
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Aquaculture Fisheries and Fish Science Wang, Yiming V. Wan, Alex H.L. Krogdahl, Åshild Johnson, Mark Larsen, Thomas (13)C values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish |
topic_facet |
Aquaculture Fisheries and Fish Science |
description |
BACKGROUND: Stable isotope analysis of single amino acids (AA) is usually applied in food web studies for tracing biosynthetic origins of AA carbon backbones and establishing trophic positions of consumers, but the method is also showing promise for characterizing quantity and quality of dietary lipids and carbohydrates. METHODS: To investigate whether changes in high- and low-digestible carbohydrates affect δ(13)C values of glycolytic AA, i.e., AA carbon backbones sourced from the glycolytic pathway, we compared Atlantic salmon (Salmo salar) from a feeding experiment with and without dietary inclusion of the red macroalga Palmaria palmata. The Control and experimental diets had similar relative proportions of macronutrients, but their ingredients differed; in the experimental treatment, 15% Palmaria inclusion substituted proteins from fishmeal and carbohydrates from corn starch. RESULTS: We found that (13)C values of the glycolytic AA were highly sensitive to substitution of corn starch with Palmaria. The δ(13)C offsets of glycolytic AA between salmon and their diets were significantly greater in the Palmaria inclusion than Control treatment. This greater offset can be attributed to the different utilization of high- vs. low-digestible carbohydrate sources, i.e., corn starch vs. Palmaria, in the two treatments, and metabolic routing of dietary lipids. In addition, similar δ(13)C values of essential AA between treatments indicate similar nutrient assimilation efficiency for all terrestrial (pea protein concentrate and wheat gluten meal) and marine (fishmeal and red alga) derived protein sources. These results show that δ(13)C(AA) analysis is a promising tool for improving our understanding of how carnivorous fish utilize macronutrient and route metabolic intermediates to tissue. |
format |
Text |
author |
Wang, Yiming V. Wan, Alex H.L. Krogdahl, Åshild Johnson, Mark Larsen, Thomas |
author_facet |
Wang, Yiming V. Wan, Alex H.L. Krogdahl, Åshild Johnson, Mark Larsen, Thomas |
author_sort |
Wang, Yiming V. |
title |
(13)C values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish |
title_short |
(13)C values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish |
title_full |
(13)C values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish |
title_fullStr |
(13)C values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish |
title_full_unstemmed |
(13)C values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish |
title_sort |
(13)c values of glycolytic amino acids as indicators of carbohydrate utilization in carnivorous fish |
publisher |
PeerJ Inc. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754727/ https://doi.org/10.7717/peerj.7701 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754727/ http://dx.doi.org/10.7717/peerj.7701 |
op_rights |
©2019 Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
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CC-BY |
op_doi |
https://doi.org/10.7717/peerj.7701 |
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