“Water-like” Dual-Functionalized Ionic Liquids for Enzyme Activation
[Image: see text] By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH(3)(OCH(2)CH(2))(n)-Im-t-BuOH][Tf(2)N]). This unique combination of the “water-like” structure en...
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ftpubmed:oai:pubmedcentral.nih.gov:6751713 2023-05-15T13:38:01+02:00 “Water-like” Dual-Functionalized Ionic Liquids for Enzyme Activation Zhao, Hua Harter, Glenn A. Martin, Caden J. 2019-09-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751713/ http://www.ncbi.nlm.nih.gov/pubmed/31552369 https://doi.org/10.1021/acsomega.9b02118 en eng American Chemical Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751713/ http://www.ncbi.nlm.nih.gov/pubmed/31552369 http://dx.doi.org/10.1021/acsomega.9b02118 Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. CC-BY-NC-ND Text 2019 ftpubmed https://doi.org/10.1021/acsomega.9b02118 2019-09-29T00:20:43Z [Image: see text] By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH(3)(OCH(2)CH(2))(n)-Im-t-BuOH][Tf(2)N]). This unique combination of the “water-like” structure enabled very high transesterification (synthetic) activities for immobilized lipase B from Candida antarctica, which are up to 2–4 folds higher than nonfunctionalized “classical” ionic liquids (such as [BMIM][Tf(2)N]) and up to 40–100% higher than diisopropyl ether and tert-butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized ILs showed high thermal stabilities, which are comparable with diisopropyl ether but much higher than tert-butanol. Text Antarc* Antarctica PubMed Central (PMC) ACS Omega 4 12 15234 15239 |
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[Image: see text] By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH(3)(OCH(2)CH(2))(n)-Im-t-BuOH][Tf(2)N]). This unique combination of the “water-like” structure enabled very high transesterification (synthetic) activities for immobilized lipase B from Candida antarctica, which are up to 2–4 folds higher than nonfunctionalized “classical” ionic liquids (such as [BMIM][Tf(2)N]) and up to 40–100% higher than diisopropyl ether and tert-butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized ILs showed high thermal stabilities, which are comparable with diisopropyl ether but much higher than tert-butanol. |
format |
Text |
author |
Zhao, Hua Harter, Glenn A. Martin, Caden J. |
spellingShingle |
Zhao, Hua Harter, Glenn A. Martin, Caden J. “Water-like” Dual-Functionalized Ionic Liquids for Enzyme Activation |
author_facet |
Zhao, Hua Harter, Glenn A. Martin, Caden J. |
author_sort |
Zhao, Hua |
title |
“Water-like” Dual-Functionalized Ionic Liquids for Enzyme Activation |
title_short |
“Water-like” Dual-Functionalized Ionic Liquids for Enzyme Activation |
title_full |
“Water-like” Dual-Functionalized Ionic Liquids for Enzyme Activation |
title_fullStr |
“Water-like” Dual-Functionalized Ionic Liquids for Enzyme Activation |
title_full_unstemmed |
“Water-like” Dual-Functionalized Ionic Liquids for Enzyme Activation |
title_sort |
“water-like” dual-functionalized ionic liquids for enzyme activation |
publisher |
American Chemical Society |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751713/ http://www.ncbi.nlm.nih.gov/pubmed/31552369 https://doi.org/10.1021/acsomega.9b02118 |
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Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751713/ http://www.ncbi.nlm.nih.gov/pubmed/31552369 http://dx.doi.org/10.1021/acsomega.9b02118 |
op_rights |
Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
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CC-BY-NC-ND |
op_doi |
https://doi.org/10.1021/acsomega.9b02118 |
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ACS Omega |
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12 |
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