Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin
The effect of collagen peptides (CPs) in intestinal mucosal protection has been approved in both cell and animal models. However, its structure–activity relationship and efficient peptide sequences are unclear, which hinders the in-depth study of its action mechanism and relative nutraceuticals and...
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ftpubmed:oai:pubmedcentral.nih.gov:6723256 2023-05-15T13:09:21+02:00 Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin Song, Wenkui Chen, Qianru Wang, Ying Han, Yan Zhang, Hongwei Li, Bo Yu, Guangli 2019-07-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723256/ http://www.ncbi.nlm.nih.gov/pubmed/31370332 https://doi.org/10.3390/md17080450 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723256/ http://www.ncbi.nlm.nih.gov/pubmed/31370332 http://dx.doi.org/10.3390/md17080450 © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2019 ftpubmed https://doi.org/10.3390/md17080450 2019-09-15T00:22:26Z The effect of collagen peptides (CPs) in intestinal mucosal protection has been approved in both cell and animal models. However, its structure–activity relationship and efficient peptide sequences are unclear, which hinders the in-depth study of its action mechanism and relative nutraceuticals and pharmaceuticals development. In this work, size exclusion chromatography, cation-exchange chromatography, and RP-HPLC were used to separate Alaska pollock skin-derived collagen hydrolysates based on their molecular weight, charge property, and hydrophobicity. The intestinal epithelial barrier function (IEBF) protective effect of separated peptide fractions were evaluated by tumor necrosis factor (TNF)-α-induced Caco-2 cell model. Results indicated that lower molecular weight (500–1000 Da) and higher hydrophilicity of CPs were related to better IEBF protective effect. Two high-efficiency IEBF protective peptide sequences, GPSGPQGSR and GPSGLLGPK with the corresponding molecular weights of 841.41 Da and 824.38 Da, were subsequently identified by UPLC-QToF-MS/MS. Their IEBF protective ability are comparable or even better than the currently used intestinal health supplements glutamine and arginine. The present findings suggested that the hydrophilic CPs, with molecular weight between 500 Da to 1000 Da, should be preferred in IEBF protective peptides preparation. GPSGPQGSR and GPSGLLGPK might have the potential of being IEBF protective ingredients used in intestinal health supplements and drugs. Text alaska pollock Alaska PubMed Central (PMC) Marine Drugs 17 8 450 |
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Article Song, Wenkui Chen, Qianru Wang, Ying Han, Yan Zhang, Hongwei Li, Bo Yu, Guangli Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
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Article |
description |
The effect of collagen peptides (CPs) in intestinal mucosal protection has been approved in both cell and animal models. However, its structure–activity relationship and efficient peptide sequences are unclear, which hinders the in-depth study of its action mechanism and relative nutraceuticals and pharmaceuticals development. In this work, size exclusion chromatography, cation-exchange chromatography, and RP-HPLC were used to separate Alaska pollock skin-derived collagen hydrolysates based on their molecular weight, charge property, and hydrophobicity. The intestinal epithelial barrier function (IEBF) protective effect of separated peptide fractions were evaluated by tumor necrosis factor (TNF)-α-induced Caco-2 cell model. Results indicated that lower molecular weight (500–1000 Da) and higher hydrophilicity of CPs were related to better IEBF protective effect. Two high-efficiency IEBF protective peptide sequences, GPSGPQGSR and GPSGLLGPK with the corresponding molecular weights of 841.41 Da and 824.38 Da, were subsequently identified by UPLC-QToF-MS/MS. Their IEBF protective ability are comparable or even better than the currently used intestinal health supplements glutamine and arginine. The present findings suggested that the hydrophilic CPs, with molecular weight between 500 Da to 1000 Da, should be preferred in IEBF protective peptides preparation. GPSGPQGSR and GPSGLLGPK might have the potential of being IEBF protective ingredients used in intestinal health supplements and drugs. |
format |
Text |
author |
Song, Wenkui Chen, Qianru Wang, Ying Han, Yan Zhang, Hongwei Li, Bo Yu, Guangli |
author_facet |
Song, Wenkui Chen, Qianru Wang, Ying Han, Yan Zhang, Hongwei Li, Bo Yu, Guangli |
author_sort |
Song, Wenkui |
title |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_short |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_full |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_fullStr |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_full_unstemmed |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_sort |
identification and structure–activity relationship of intestinal epithelial barrier function protective collagen peptides from alaska pollock skin |
publisher |
MDPI |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723256/ http://www.ncbi.nlm.nih.gov/pubmed/31370332 https://doi.org/10.3390/md17080450 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723256/ http://www.ncbi.nlm.nih.gov/pubmed/31370332 http://dx.doi.org/10.3390/md17080450 |
op_rights |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md17080450 |
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Marine Drugs |
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450 |
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1766173785735561216 |