Molecular Detection of Babesia spp. (Apicomplexa: Piroplasma) in Free-Ranging Canids and Mustelids From Southern Italy
Babesiosis is an emerging tick-borne disease caused by apicomplexan parasites with widespread geographical distribution and various wildlife species as reservoir hosts. The aims of this study were to investigate the prevalence and assess the role of free-ranging canids and mustelids in the maintenan...
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ftpubmed:oai:pubmedcentral.nih.gov:6716145 2023-05-15T15:50:47+02:00 Molecular Detection of Babesia spp. (Apicomplexa: Piroplasma) in Free-Ranging Canids and Mustelids From Southern Italy Santoro, Mario Auriemma, Clementina Lucibelli, Maria Gabriella Borriello, Giorgia D'Alessio, Nicola Sgroi, Giovanni Veneziano, Vincenzo Galiero, Giorgio Fusco, Giovanna 2019-08-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716145/ https://doi.org/10.3389/fvets.2019.00269 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716145/ http://dx.doi.org/10.3389/fvets.2019.00269 Copyright © 2019 Santoro, Auriemma, Lucibelli, Borriello, D'Alessio, Sgroi, Veneziano, Galiero and Fusco. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Veterinary Science Text 2019 ftpubmed https://doi.org/10.3389/fvets.2019.00269 2019-09-29T00:12:12Z Babesiosis is an emerging tick-borne disease caused by apicomplexan parasites with widespread geographical distribution and various wildlife species as reservoir hosts. The aims of this study were to investigate the prevalence and assess the role of free-ranging canids and mustelids in the maintenance of Babesia spp. in southern Italy. PCR analysis of splenic samples targeting the 18S rRNA gene revealed the presence of Babesia spp. in 36 of 82 (43.9%) red foxes (Vulpes vulpes) including 29 (58%) from Campania region and seven (21.8%) from Calabria region, in seven of 13 (53.8%) Eurasian badgers (Meles meles), and in one of 13 (7.7%) gray wolves (Canis lupus). Samples from other host species including 9 Eurasian otters (Lutra lutra), 1 stone marten (Martes foina), 1 least weasel (Mustela nivalis), and 1 European polecat (Mustela putorius) tested Babesia spp. negative. Sequence analysis of the 18S rRNA gene demonstrated the presence of B. vulpes in the red fox and two sequence types of badger-associated Babesia spp. in the Eurasian badger. The Babesia sp. sequence detected in the gray wolf was identical to a badger-associated Babesia sp. This study shows that the number of Babesia spp. infecting free-ranging carnivores in Italy is higher than currently believed, and suggests that these hosts may play an important role in the maintenance of the sylvatic cycle of these parasites. It is the first report of badger-associated Babesia spp. in Italy and in a gray wolf. Text Canis lupus gray wolf Lutra lutra PubMed Central (PMC) Frontiers in Veterinary Science 6 |
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Veterinary Science Santoro, Mario Auriemma, Clementina Lucibelli, Maria Gabriella Borriello, Giorgia D'Alessio, Nicola Sgroi, Giovanni Veneziano, Vincenzo Galiero, Giorgio Fusco, Giovanna Molecular Detection of Babesia spp. (Apicomplexa: Piroplasma) in Free-Ranging Canids and Mustelids From Southern Italy |
topic_facet |
Veterinary Science |
description |
Babesiosis is an emerging tick-borne disease caused by apicomplexan parasites with widespread geographical distribution and various wildlife species as reservoir hosts. The aims of this study were to investigate the prevalence and assess the role of free-ranging canids and mustelids in the maintenance of Babesia spp. in southern Italy. PCR analysis of splenic samples targeting the 18S rRNA gene revealed the presence of Babesia spp. in 36 of 82 (43.9%) red foxes (Vulpes vulpes) including 29 (58%) from Campania region and seven (21.8%) from Calabria region, in seven of 13 (53.8%) Eurasian badgers (Meles meles), and in one of 13 (7.7%) gray wolves (Canis lupus). Samples from other host species including 9 Eurasian otters (Lutra lutra), 1 stone marten (Martes foina), 1 least weasel (Mustela nivalis), and 1 European polecat (Mustela putorius) tested Babesia spp. negative. Sequence analysis of the 18S rRNA gene demonstrated the presence of B. vulpes in the red fox and two sequence types of badger-associated Babesia spp. in the Eurasian badger. The Babesia sp. sequence detected in the gray wolf was identical to a badger-associated Babesia sp. This study shows that the number of Babesia spp. infecting free-ranging carnivores in Italy is higher than currently believed, and suggests that these hosts may play an important role in the maintenance of the sylvatic cycle of these parasites. It is the first report of badger-associated Babesia spp. in Italy and in a gray wolf. |
format |
Text |
author |
Santoro, Mario Auriemma, Clementina Lucibelli, Maria Gabriella Borriello, Giorgia D'Alessio, Nicola Sgroi, Giovanni Veneziano, Vincenzo Galiero, Giorgio Fusco, Giovanna |
author_facet |
Santoro, Mario Auriemma, Clementina Lucibelli, Maria Gabriella Borriello, Giorgia D'Alessio, Nicola Sgroi, Giovanni Veneziano, Vincenzo Galiero, Giorgio Fusco, Giovanna |
author_sort |
Santoro, Mario |
title |
Molecular Detection of Babesia spp. (Apicomplexa: Piroplasma) in Free-Ranging Canids and Mustelids From Southern Italy |
title_short |
Molecular Detection of Babesia spp. (Apicomplexa: Piroplasma) in Free-Ranging Canids and Mustelids From Southern Italy |
title_full |
Molecular Detection of Babesia spp. (Apicomplexa: Piroplasma) in Free-Ranging Canids and Mustelids From Southern Italy |
title_fullStr |
Molecular Detection of Babesia spp. (Apicomplexa: Piroplasma) in Free-Ranging Canids and Mustelids From Southern Italy |
title_full_unstemmed |
Molecular Detection of Babesia spp. (Apicomplexa: Piroplasma) in Free-Ranging Canids and Mustelids From Southern Italy |
title_sort |
molecular detection of babesia spp. (apicomplexa: piroplasma) in free-ranging canids and mustelids from southern italy |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716145/ https://doi.org/10.3389/fvets.2019.00269 |
genre |
Canis lupus gray wolf Lutra lutra |
genre_facet |
Canis lupus gray wolf Lutra lutra |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716145/ http://dx.doi.org/10.3389/fvets.2019.00269 |
op_rights |
Copyright © 2019 Santoro, Auriemma, Lucibelli, Borriello, D'Alessio, Sgroi, Veneziano, Galiero and Fusco. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fvets.2019.00269 |
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Frontiers in Veterinary Science |
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6 |
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1766385801416933376 |