Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene
BACKGROUND: Candidatus Neoehrlichia mikurensis is an emerging tick-borne pathogen. It is widely distributed in Ixodes ricinus ticks in Europe, but knowledge of its distribution in Norway, where I. ricinus reaches its northern limit, is limited. In this study we have developed a real time PCR test fo...
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ftpubmed:oai:pubmedcentral.nih.gov:6714093 2023-05-15T14:59:20+02:00 Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene Jenkins, Andrew Raasok, Cecilie Pedersen, Benedikte N. Jensen, Kristine Andreassen, Åshild Soleng, Arnulf Edgar, Kristin Skarsfjord Lindstedt, Heidi Heggen Kjelland, Vivian Stuen, Snorre Hvidsten, Dag Kristiansen, Bjørn-Erik 2019-08-28 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714093/ http://www.ncbi.nlm.nih.gov/pubmed/31462211 https://doi.org/10.1186/s12866-019-1502-y en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714093/ http://www.ncbi.nlm.nih.gov/pubmed/31462211 http://dx.doi.org/10.1186/s12866-019-1502-y © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Research Article Text 2019 ftpubmed https://doi.org/10.1186/s12866-019-1502-y 2019-09-08T00:40:59Z BACKGROUND: Candidatus Neoehrlichia mikurensis is an emerging tick-borne pathogen. It is widely distributed in Ixodes ricinus ticks in Europe, but knowledge of its distribution in Norway, where I. ricinus reaches its northern limit, is limited. In this study we have developed a real time PCR test for Ca. N. mikurensis and used it to investigate the distribution of Ca. N. mikurensis in Norway. RESULTS: Real time PCR targeting the groEL gene was developed and shown to be highly sensitive. It was used to detect Ca. N. mikurensis in 1651 I. ricinus nymphs and adults collected from twelve locations in Norway, from the eastern Oslo Fjord in the south to near the Arctic Circle in the north. The overall prevalence was 6.5% and varied locally between 0 and 16%. Prevalence in adults and nymphs was similar, suggesting that ticks acquire Ca. N. mikurensis predominantly during their first blood meal. In addition, 123 larvae were investigated; Ca. N. mikurensis was not found in larvae, suggesting that transovarial transmission is rare or absent. Sequence analysis suggests that a single variant dominates in Norway. CONCLUSIONS: Ca. N. mikurensis is widespread and common in ticks in Norway and reaches up to their northern limit near the Arctic Circle. Ticks appear to acquire Ca. N. mikurensis during their first blood meal. No evidence for transovarial transmission was found. Text Arctic PubMed Central (PMC) Arctic Norway BMC Microbiology 19 1 |
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Research Article Jenkins, Andrew Raasok, Cecilie Pedersen, Benedikte N. Jensen, Kristine Andreassen, Åshild Soleng, Arnulf Edgar, Kristin Skarsfjord Lindstedt, Heidi Heggen Kjelland, Vivian Stuen, Snorre Hvidsten, Dag Kristiansen, Bjørn-Erik Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
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Research Article |
description |
BACKGROUND: Candidatus Neoehrlichia mikurensis is an emerging tick-borne pathogen. It is widely distributed in Ixodes ricinus ticks in Europe, but knowledge of its distribution in Norway, where I. ricinus reaches its northern limit, is limited. In this study we have developed a real time PCR test for Ca. N. mikurensis and used it to investigate the distribution of Ca. N. mikurensis in Norway. RESULTS: Real time PCR targeting the groEL gene was developed and shown to be highly sensitive. It was used to detect Ca. N. mikurensis in 1651 I. ricinus nymphs and adults collected from twelve locations in Norway, from the eastern Oslo Fjord in the south to near the Arctic Circle in the north. The overall prevalence was 6.5% and varied locally between 0 and 16%. Prevalence in adults and nymphs was similar, suggesting that ticks acquire Ca. N. mikurensis predominantly during their first blood meal. In addition, 123 larvae were investigated; Ca. N. mikurensis was not found in larvae, suggesting that transovarial transmission is rare or absent. Sequence analysis suggests that a single variant dominates in Norway. CONCLUSIONS: Ca. N. mikurensis is widespread and common in ticks in Norway and reaches up to their northern limit near the Arctic Circle. Ticks appear to acquire Ca. N. mikurensis during their first blood meal. No evidence for transovarial transmission was found. |
format |
Text |
author |
Jenkins, Andrew Raasok, Cecilie Pedersen, Benedikte N. Jensen, Kristine Andreassen, Åshild Soleng, Arnulf Edgar, Kristin Skarsfjord Lindstedt, Heidi Heggen Kjelland, Vivian Stuen, Snorre Hvidsten, Dag Kristiansen, Bjørn-Erik |
author_facet |
Jenkins, Andrew Raasok, Cecilie Pedersen, Benedikte N. Jensen, Kristine Andreassen, Åshild Soleng, Arnulf Edgar, Kristin Skarsfjord Lindstedt, Heidi Heggen Kjelland, Vivian Stuen, Snorre Hvidsten, Dag Kristiansen, Bjørn-Erik |
author_sort |
Jenkins, Andrew |
title |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_short |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_full |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_fullStr |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_full_unstemmed |
Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene |
title_sort |
detection of candidatus neoehrlichia mikurensis in norway up to the northern limit of ixodes ricinus distribution using a novel real time pcr test targeting the groel gene |
publisher |
BioMed Central |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714093/ http://www.ncbi.nlm.nih.gov/pubmed/31462211 https://doi.org/10.1186/s12866-019-1502-y |
geographic |
Arctic Norway |
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Arctic Norway |
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Arctic |
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Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714093/ http://www.ncbi.nlm.nih.gov/pubmed/31462211 http://dx.doi.org/10.1186/s12866-019-1502-y |
op_rights |
© The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s12866-019-1502-y |
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BMC Microbiology |
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19 |
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1766331446164717568 |