Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate
Background/Aim: Biocompatibility of a novel and more stable hydroxyapatite (HA)-dispersed titanium (Ti)-based composite was investigated, using the mouse osteoblast precursor cell line MC3T3-E1. Materials and Methods: Surface of powders and plates was observed by scanning electron microscopy. Distri...
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International Institute of Anticancer Research
2019
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689340/ http://www.ncbi.nlm.nih.gov/pubmed/31280194 https://doi.org/10.21873/invivo.11575 |
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ftpubmed:oai:pubmedcentral.nih.gov:6689340 2023-05-15T15:52:46+02:00 Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate KOBAYASHI, MASAHIKO NIHONMATSU, SHOICHI OKAWARA, TAKAHIRO ONUKI, HIROYUKI SAKAGAMI, HIROSHI NAKAJIMA, HIROSHI TAKEISHI, HIROYUKU SHIMADA, JUN 2019-07-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689340/ http://www.ncbi.nlm.nih.gov/pubmed/31280194 https://doi.org/10.21873/invivo.11575 en eng International Institute of Anticancer Research http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689340/ http://www.ncbi.nlm.nih.gov/pubmed/31280194 http://dx.doi.org/10.21873/invivo.11575 Copyright 2019, International Institute of Anticancer Research Research Article Text 2019 ftpubmed https://doi.org/10.21873/invivo.11575 2019-08-18T00:52:01Z Background/Aim: Biocompatibility of a novel and more stable hydroxyapatite (HA)-dispersed titanium (Ti)-based composite was investigated, using the mouse osteoblast precursor cell line MC3T3-E1. Materials and Methods: Surface of powders and plates was observed by scanning electron microscopy. Distribution of calcium and phosphorus on the surface of the composite was evaluated by an electron beam microanalyzer. Crystal structure was analyzed by X-ray diffractometer. Cell viability was determined by WST-1 assay. Results: HA was stable against the compressive force, shearing stress and sintering heat at 800˚C, but it slightly decomposed at 1100˚C. With the increase of HA in the composites, the adhesion/proliferation of MC3T3-E1 cells was reduced. The growth inhibition by HA does not seem to be due to materials released from the plate, but rather to the contact to the surface of the plate. Sintering of the HA plate at 1100˚C increased the number of attached viable cells. On the other hand, culturing on the synthesized calcium phosphate (apatite containing carbonic acid) increased the number of attached cells to a greater extent. Conclusion: HA inhibits the growth of osteoblastic cells, but sintering at 1100˚C changes the surface properties of the composite so as to stimulate cell growth. Text Carbonic acid PubMed Central (PMC) In Vivo 33 4 1067 1079 |
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Research Article KOBAYASHI, MASAHIKO NIHONMATSU, SHOICHI OKAWARA, TAKAHIRO ONUKI, HIROYUKI SAKAGAMI, HIROSHI NAKAJIMA, HIROSHI TAKEISHI, HIROYUKU SHIMADA, JUN Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate |
topic_facet |
Research Article |
description |
Background/Aim: Biocompatibility of a novel and more stable hydroxyapatite (HA)-dispersed titanium (Ti)-based composite was investigated, using the mouse osteoblast precursor cell line MC3T3-E1. Materials and Methods: Surface of powders and plates was observed by scanning electron microscopy. Distribution of calcium and phosphorus on the surface of the composite was evaluated by an electron beam microanalyzer. Crystal structure was analyzed by X-ray diffractometer. Cell viability was determined by WST-1 assay. Results: HA was stable against the compressive force, shearing stress and sintering heat at 800˚C, but it slightly decomposed at 1100˚C. With the increase of HA in the composites, the adhesion/proliferation of MC3T3-E1 cells was reduced. The growth inhibition by HA does not seem to be due to materials released from the plate, but rather to the contact to the surface of the plate. Sintering of the HA plate at 1100˚C increased the number of attached viable cells. On the other hand, culturing on the synthesized calcium phosphate (apatite containing carbonic acid) increased the number of attached cells to a greater extent. Conclusion: HA inhibits the growth of osteoblastic cells, but sintering at 1100˚C changes the surface properties of the composite so as to stimulate cell growth. |
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Text |
author |
KOBAYASHI, MASAHIKO NIHONMATSU, SHOICHI OKAWARA, TAKAHIRO ONUKI, HIROYUKI SAKAGAMI, HIROSHI NAKAJIMA, HIROSHI TAKEISHI, HIROYUKU SHIMADA, JUN |
author_facet |
KOBAYASHI, MASAHIKO NIHONMATSU, SHOICHI OKAWARA, TAKAHIRO ONUKI, HIROYUKI SAKAGAMI, HIROSHI NAKAJIMA, HIROSHI TAKEISHI, HIROYUKU SHIMADA, JUN |
author_sort |
KOBAYASHI, MASAHIKO |
title |
Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate |
title_short |
Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate |
title_full |
Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate |
title_fullStr |
Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate |
title_full_unstemmed |
Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate |
title_sort |
adhesion and proliferation of osteoblastic cells on hydroxyapatite-dispersed ti-based composite plate |
publisher |
International Institute of Anticancer Research |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689340/ http://www.ncbi.nlm.nih.gov/pubmed/31280194 https://doi.org/10.21873/invivo.11575 |
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Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689340/ http://www.ncbi.nlm.nih.gov/pubmed/31280194 http://dx.doi.org/10.21873/invivo.11575 |
op_rights |
Copyright 2019, International Institute of Anticancer Research |
op_doi |
https://doi.org/10.21873/invivo.11575 |
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In Vivo |
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33 |
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4 |
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1067 |
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1079 |
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