Role of Anti–Carbamylated Protein Antibodies Compared to Anti–Citrullinated Protein Antibodies in Indigenous North Americans With Rheumatoid Arthritis, Their First‐Degree Relatives, and Healthy Controls
OBJECTIVE: Rheumatoid arthritis (RA) is characterized by the presence of autoantibodies, including seropositivity for rheumatoid factor (RF) and anti–citrullinated protein antibodies (ACPAs). In addition, antibodies to carbamylated proteins (anti‐CarP) are present in patients with RA and are associa...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680251/ http://www.ncbi.nlm.nih.gov/pubmed/26946484 https://doi.org/10.1002/art.39664 |
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ftpubmed:oai:pubmedcentral.nih.gov:6680251 2023-05-15T16:17:13+02:00 Role of Anti–Carbamylated Protein Antibodies Compared to Anti–Citrullinated Protein Antibodies in Indigenous North Americans With Rheumatoid Arthritis, Their First‐Degree Relatives, and Healthy Controls Koppejan, H. Trouw, L. A. Sokolove, J. Lahey, L. J. Huizinga, T. J. W. Smolik, I. A. Robinson, D. B. El‐Gabalawy, H. S. Toes, R. E. M. Hitchon, C. A. 2016-08-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680251/ http://www.ncbi.nlm.nih.gov/pubmed/26946484 https://doi.org/10.1002/art.39664 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680251/ http://www.ncbi.nlm.nih.gov/pubmed/26946484 http://dx.doi.org/10.1002/art.39664 © 2016 The Authors. Arthritis & Rheumatology published by Wiley Periodicals, Inc. on behalf of the American College of Rheumatology. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. CC-BY-NC-ND Rheumatoid Arthritis Text 2016 ftpubmed https://doi.org/10.1002/art.39664 2019-08-18T00:39:12Z OBJECTIVE: Rheumatoid arthritis (RA) is characterized by the presence of autoantibodies, including seropositivity for rheumatoid factor (RF) and anti–citrullinated protein antibodies (ACPAs). In addition, antibodies to carbamylated proteins (anti‐CarP) are present in patients with RA and are associated with joint damage. This study was undertaken to assess the presence of anti‐CarP antibodies in indigenous North Americans (First Nations [FN] populations) with RA compared to their at‐risk first‐degree relatives (FDRs) and healthy controls. METHODS: Anti‐CarP IgG and ACPAs (specifically, anti–cyclic citrullinated peptide [anti‐CCP] antibodies) were measured by enzyme‐linked immunosorbent assay in the sera of FN patients with RA (n = 95), their unaffected FDRs (n = 109), and healthy FN controls (n = 85). Antibodies to additional citrullinated peptides were measured using a multiplex ACPA array, and the number of peptides recognized was reported as an ACPA score. Groups were compared using the chi‐square test and Mann‐Whitney U test. Associations between RA and seropositivity for RF, ACPAs, and anti‐CarP antibodies were determined by logistic regression. RESULTS: Anti‐CarP antibodies were more frequent in FN patients with RA (44.3%) compared to FDRs (18.3%) and FN controls (4.7%) (both P < 0.0001 versus RA). Moreover, anti‐CarP antibodies were more frequent in FDRs than in FN controls (P = 0.008). The ACPA score was higher in anti‐CCP–positive FN patients with RA than in anti‐CCP–positive FN FDRs (median score 7 [interquartile range (IQR) 7] versus median score 1 [IQR 4]; P = 0.04). The association with RA was strongest when all 3 autoantibodies (RF, anti‐CCP, and anti‐CarP) were present in the patients’ serum (odds ratio 194, 95% confidence interval 23–1,609, P < 0.0001). CONCLUSION: Anti‐CarP antibodies are prevalent in FN patients with RA and also more common in their at‐risk FDRs compared to healthy controls. The results indicate an association of RF, ACPAs, and anti‐CarP with RA that is strongest when all ... Text First Nations PubMed Central (PMC) Arthritis & Rheumatology 68 9 2090 2098 |
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English |
topic |
Rheumatoid Arthritis |
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Rheumatoid Arthritis Koppejan, H. Trouw, L. A. Sokolove, J. Lahey, L. J. Huizinga, T. J. W. Smolik, I. A. Robinson, D. B. El‐Gabalawy, H. S. Toes, R. E. M. Hitchon, C. A. Role of Anti–Carbamylated Protein Antibodies Compared to Anti–Citrullinated Protein Antibodies in Indigenous North Americans With Rheumatoid Arthritis, Their First‐Degree Relatives, and Healthy Controls |
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Rheumatoid Arthritis |
description |
OBJECTIVE: Rheumatoid arthritis (RA) is characterized by the presence of autoantibodies, including seropositivity for rheumatoid factor (RF) and anti–citrullinated protein antibodies (ACPAs). In addition, antibodies to carbamylated proteins (anti‐CarP) are present in patients with RA and are associated with joint damage. This study was undertaken to assess the presence of anti‐CarP antibodies in indigenous North Americans (First Nations [FN] populations) with RA compared to their at‐risk first‐degree relatives (FDRs) and healthy controls. METHODS: Anti‐CarP IgG and ACPAs (specifically, anti–cyclic citrullinated peptide [anti‐CCP] antibodies) were measured by enzyme‐linked immunosorbent assay in the sera of FN patients with RA (n = 95), their unaffected FDRs (n = 109), and healthy FN controls (n = 85). Antibodies to additional citrullinated peptides were measured using a multiplex ACPA array, and the number of peptides recognized was reported as an ACPA score. Groups were compared using the chi‐square test and Mann‐Whitney U test. Associations between RA and seropositivity for RF, ACPAs, and anti‐CarP antibodies were determined by logistic regression. RESULTS: Anti‐CarP antibodies were more frequent in FN patients with RA (44.3%) compared to FDRs (18.3%) and FN controls (4.7%) (both P < 0.0001 versus RA). Moreover, anti‐CarP antibodies were more frequent in FDRs than in FN controls (P = 0.008). The ACPA score was higher in anti‐CCP–positive FN patients with RA than in anti‐CCP–positive FN FDRs (median score 7 [interquartile range (IQR) 7] versus median score 1 [IQR 4]; P = 0.04). The association with RA was strongest when all 3 autoantibodies (RF, anti‐CCP, and anti‐CarP) were present in the patients’ serum (odds ratio 194, 95% confidence interval 23–1,609, P < 0.0001). CONCLUSION: Anti‐CarP antibodies are prevalent in FN patients with RA and also more common in their at‐risk FDRs compared to healthy controls. The results indicate an association of RF, ACPAs, and anti‐CarP with RA that is strongest when all ... |
format |
Text |
author |
Koppejan, H. Trouw, L. A. Sokolove, J. Lahey, L. J. Huizinga, T. J. W. Smolik, I. A. Robinson, D. B. El‐Gabalawy, H. S. Toes, R. E. M. Hitchon, C. A. |
author_facet |
Koppejan, H. Trouw, L. A. Sokolove, J. Lahey, L. J. Huizinga, T. J. W. Smolik, I. A. Robinson, D. B. El‐Gabalawy, H. S. Toes, R. E. M. Hitchon, C. A. |
author_sort |
Koppejan, H. |
title |
Role of Anti–Carbamylated Protein Antibodies Compared to Anti–Citrullinated Protein Antibodies in Indigenous North Americans With Rheumatoid Arthritis, Their First‐Degree Relatives, and Healthy Controls |
title_short |
Role of Anti–Carbamylated Protein Antibodies Compared to Anti–Citrullinated Protein Antibodies in Indigenous North Americans With Rheumatoid Arthritis, Their First‐Degree Relatives, and Healthy Controls |
title_full |
Role of Anti–Carbamylated Protein Antibodies Compared to Anti–Citrullinated Protein Antibodies in Indigenous North Americans With Rheumatoid Arthritis, Their First‐Degree Relatives, and Healthy Controls |
title_fullStr |
Role of Anti–Carbamylated Protein Antibodies Compared to Anti–Citrullinated Protein Antibodies in Indigenous North Americans With Rheumatoid Arthritis, Their First‐Degree Relatives, and Healthy Controls |
title_full_unstemmed |
Role of Anti–Carbamylated Protein Antibodies Compared to Anti–Citrullinated Protein Antibodies in Indigenous North Americans With Rheumatoid Arthritis, Their First‐Degree Relatives, and Healthy Controls |
title_sort |
role of anti–carbamylated protein antibodies compared to anti–citrullinated protein antibodies in indigenous north americans with rheumatoid arthritis, their first‐degree relatives, and healthy controls |
publisher |
John Wiley and Sons Inc. |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680251/ http://www.ncbi.nlm.nih.gov/pubmed/26946484 https://doi.org/10.1002/art.39664 |
genre |
First Nations |
genre_facet |
First Nations |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680251/ http://www.ncbi.nlm.nih.gov/pubmed/26946484 http://dx.doi.org/10.1002/art.39664 |
op_rights |
© 2016 The Authors. Arthritis & Rheumatology published by Wiley Periodicals, Inc. on behalf of the American College of Rheumatology. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1002/art.39664 |
container_title |
Arthritis & Rheumatology |
container_volume |
68 |
container_issue |
9 |
container_start_page |
2090 |
op_container_end_page |
2098 |
_version_ |
1766003058721947648 |