Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile
BACKGROUND: Rattus rattus is a widely distributed, invasive species that presents an important role in disease transmission, either directly or through vector arthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella, which can ca...
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ftpubmed:oai:pubmedcentral.nih.gov:6679904 2023-05-15T18:05:12+02:00 Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile Moreno Salas, Lucila Espinoza-Carniglia, Mario Lizama Schmeisser, Nicol Torres, L. Gonzalo Silva-de la Fuente, María Carolina Lareschi, Marcela González-Acuña, Daniel 2019-08-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679904/ http://www.ncbi.nlm.nih.gov/pubmed/31396444 https://doi.org/10.7717/peerj.7371 en eng PeerJ Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679904/ http://www.ncbi.nlm.nih.gov/pubmed/31396444 http://dx.doi.org/10.7717/peerj.7371 © 2019 Moreno Salas et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. CC-BY Parasitology Text 2019 ftpubmed https://doi.org/10.7717/peerj.7371 2019-08-18T00:38:36Z BACKGROUND: Rattus rattus is a widely distributed, invasive species that presents an important role in disease transmission, either directly or through vector arthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella, which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. METHODS: In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis. RESULTS: Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species (n = 55), and it also presented a high prevalence of Bartonella DNA (P% = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB), followed by cities (14.8% gltA and 11.1% rpoB) and wild areas (7.4% gltA and 14.8% rpoB). The BLAST analysis showed a high similitude ... Text Rattus rattus PubMed Central (PMC) PeerJ 7 e7371 |
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Parasitology |
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Parasitology Moreno Salas, Lucila Espinoza-Carniglia, Mario Lizama Schmeisser, Nicol Torres, L. Gonzalo Silva-de la Fuente, María Carolina Lareschi, Marcela González-Acuña, Daniel Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile |
topic_facet |
Parasitology |
description |
BACKGROUND: Rattus rattus is a widely distributed, invasive species that presents an important role in disease transmission, either directly or through vector arthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella, which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. METHODS: In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis. RESULTS: Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species (n = 55), and it also presented a high prevalence of Bartonella DNA (P% = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB), followed by cities (14.8% gltA and 11.1% rpoB) and wild areas (7.4% gltA and 14.8% rpoB). The BLAST analysis showed a high similitude ... |
format |
Text |
author |
Moreno Salas, Lucila Espinoza-Carniglia, Mario Lizama Schmeisser, Nicol Torres, L. Gonzalo Silva-de la Fuente, María Carolina Lareschi, Marcela González-Acuña, Daniel |
author_facet |
Moreno Salas, Lucila Espinoza-Carniglia, Mario Lizama Schmeisser, Nicol Torres, L. Gonzalo Silva-de la Fuente, María Carolina Lareschi, Marcela González-Acuña, Daniel |
author_sort |
Moreno Salas, Lucila |
title |
Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile |
title_short |
Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile |
title_full |
Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile |
title_fullStr |
Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile |
title_full_unstemmed |
Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile |
title_sort |
fleas of black rats (rattus rattus) as reservoir host of bartonella spp. in chile |
publisher |
PeerJ Inc. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679904/ http://www.ncbi.nlm.nih.gov/pubmed/31396444 https://doi.org/10.7717/peerj.7371 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679904/ http://www.ncbi.nlm.nih.gov/pubmed/31396444 http://dx.doi.org/10.7717/peerj.7371 |
op_rights |
© 2019 Moreno Salas et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.7717/peerj.7371 |
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e7371 |
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