Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis
The Dungeness crab is an economically and ecologically important species distributed along the North American Pacific coast. To predict how Dungeness crab may physiologically respond to future global ocean change on a molecular level, we performed untargeted metabolomic approaches on individual Dung...
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ftpubmed:oai:pubmedcentral.nih.gov:6656712 2023-05-15T17:51:17+02:00 Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis Trigg, Shelly A. McElhany, Paul Maher, Michael Perez, Danielle Busch, D. Shallin Nichols, Krista M. 2019-07-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656712/ http://www.ncbi.nlm.nih.gov/pubmed/31341175 https://doi.org/10.1038/s41598-019-46947-6 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656712/ http://www.ncbi.nlm.nih.gov/pubmed/31341175 http://dx.doi.org/10.1038/s41598-019-46947-6 © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2019 ftpubmed https://doi.org/10.1038/s41598-019-46947-6 2019-08-04T01:01:55Z The Dungeness crab is an economically and ecologically important species distributed along the North American Pacific coast. To predict how Dungeness crab may physiologically respond to future global ocean change on a molecular level, we performed untargeted metabolomic approaches on individual Dungeness crab juveniles reared in treatments that mimicked current and projected future pH and dissolved oxygen conditions. We found 94 metabolites and 127 lipids responded in a condition-specific manner, with a greater number of known compounds more strongly responding to low oxygen than low pH exposure. Pathway analysis of these compounds revealed that juveniles may respond to low oxygen through evolutionarily conserved processes including downregulating glutathione biosynthesis and upregulating glycogen storage, and may respond to low pH by increasing ATP production. Most interestingly, we found that the response of juveniles to combined low pH and low oxygen exposure was most similar to the low oxygen exposure response, indicating low oxygen may drive the physiology of juvenile crabs more than pH. Our study elucidates metabolic dynamics that expand our overall understanding of how the species might respond to future ocean conditions and provides a comprehensive dataset that could be used in future ocean acidification response studies. Text Ocean acidification PubMed Central (PMC) Pacific Scientific Reports 9 1 |
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Article Trigg, Shelly A. McElhany, Paul Maher, Michael Perez, Danielle Busch, D. Shallin Nichols, Krista M. Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis |
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The Dungeness crab is an economically and ecologically important species distributed along the North American Pacific coast. To predict how Dungeness crab may physiologically respond to future global ocean change on a molecular level, we performed untargeted metabolomic approaches on individual Dungeness crab juveniles reared in treatments that mimicked current and projected future pH and dissolved oxygen conditions. We found 94 metabolites and 127 lipids responded in a condition-specific manner, with a greater number of known compounds more strongly responding to low oxygen than low pH exposure. Pathway analysis of these compounds revealed that juveniles may respond to low oxygen through evolutionarily conserved processes including downregulating glutathione biosynthesis and upregulating glycogen storage, and may respond to low pH by increasing ATP production. Most interestingly, we found that the response of juveniles to combined low pH and low oxygen exposure was most similar to the low oxygen exposure response, indicating low oxygen may drive the physiology of juvenile crabs more than pH. Our study elucidates metabolic dynamics that expand our overall understanding of how the species might respond to future ocean conditions and provides a comprehensive dataset that could be used in future ocean acidification response studies. |
format |
Text |
author |
Trigg, Shelly A. McElhany, Paul Maher, Michael Perez, Danielle Busch, D. Shallin Nichols, Krista M. |
author_facet |
Trigg, Shelly A. McElhany, Paul Maher, Michael Perez, Danielle Busch, D. Shallin Nichols, Krista M. |
author_sort |
Trigg, Shelly A. |
title |
Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis |
title_short |
Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis |
title_full |
Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis |
title_fullStr |
Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis |
title_full_unstemmed |
Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis |
title_sort |
uncovering mechanisms of global ocean change effects on the dungeness crab (cancer magister) through metabolomics analysis |
publisher |
Nature Publishing Group UK |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656712/ http://www.ncbi.nlm.nih.gov/pubmed/31341175 https://doi.org/10.1038/s41598-019-46947-6 |
geographic |
Pacific |
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Pacific |
genre |
Ocean acidification |
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Ocean acidification |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656712/ http://www.ncbi.nlm.nih.gov/pubmed/31341175 http://dx.doi.org/10.1038/s41598-019-46947-6 |
op_rights |
© The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-46947-6 |
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Scientific Reports |
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