Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus)
Benzothiadiazole (BTH) induces resistance to the downy mildew pathogen, Peronospora sparsa, in arctic bramble, but the basis for the BTH‐induced resistance is unknown. Arctic bramble cv. Mespi was treated with BTH to study the changes in leaf proteome and to identify proteins with a putative role in...
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ftpubmed:oai:pubmedcentral.nih.gov:6640374 2023-05-15T14:54:13+02:00 Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus) HUKKANEN, ANNE KOKKO, HARRI BUCHALA, ANTONY HÄYRINEN, JUKKA KÄRENLAMPI, SIRPA 2008-08-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640374/ http://www.ncbi.nlm.nih.gov/pubmed/19019008 https://doi.org/10.1111/j.1364-3703.2008.00502.x en eng Blackwell Publishing Ltd http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640374/ http://www.ncbi.nlm.nih.gov/pubmed/19019008 http://dx.doi.org/10.1111/j.1364-3703.2008.00502.x © 2008 Blackwell publishing Ltd Original Articles Text 2008 ftpubmed https://doi.org/10.1111/j.1364-3703.2008.00502.x 2019-09-22T00:14:35Z Benzothiadiazole (BTH) induces resistance to the downy mildew pathogen, Peronospora sparsa, in arctic bramble, but the basis for the BTH‐induced resistance is unknown. Arctic bramble cv. Mespi was treated with BTH to study the changes in leaf proteome and to identify proteins with a putative role in disease resistance. First, BTH induced strong expression of one PR‐1 protein isoform, which was also induced by salicylic acid (SA). The PR‐1 was responsive to BTH and exogenous SA despite a high endogenous SA content (20–25 µg/g fresh weight), which increased to an even higher level after treatment with BTH. Secondly, a total of 792 protein spots were detected in two‐dimensional gel electrophoresis, eight proteins being detected solely in the BTH‐treated plants. BTH caused up‐ or down‐regulation of 72 and 31 proteins, respectively, of which 18 were tentatively identified by mass spectrometry. The up‐regulation of flavanone‐3‐hydroxylase, alanine aminotransferase, 1‐aminocyclopropane‐1‐carboxylate oxidase, PR‐1 and PR‐10 proteins may partly explain the BTH‐induced resistance against P. sparsa. Other proteins with changes in intensity appear to be involved in, for example, energy metabolism and protein processing. The decline in ATP synthase, triosephosphate isomerase, fructose bisphosphate aldolase and glutamine synthetase suggests that BTH causes significant changes in primary metabolism, which provides one possible explanation for the decreased vegetative growth of foliage and rhizome observed in BTH‐treated plants. Text Arctic PubMed Central (PMC) Arctic Molecular Plant Pathology 9 6 799 808 |
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Original Articles HUKKANEN, ANNE KOKKO, HARRI BUCHALA, ANTONY HÄYRINEN, JUKKA KÄRENLAMPI, SIRPA Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus) |
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Original Articles |
description |
Benzothiadiazole (BTH) induces resistance to the downy mildew pathogen, Peronospora sparsa, in arctic bramble, but the basis for the BTH‐induced resistance is unknown. Arctic bramble cv. Mespi was treated with BTH to study the changes in leaf proteome and to identify proteins with a putative role in disease resistance. First, BTH induced strong expression of one PR‐1 protein isoform, which was also induced by salicylic acid (SA). The PR‐1 was responsive to BTH and exogenous SA despite a high endogenous SA content (20–25 µg/g fresh weight), which increased to an even higher level after treatment with BTH. Secondly, a total of 792 protein spots were detected in two‐dimensional gel electrophoresis, eight proteins being detected solely in the BTH‐treated plants. BTH caused up‐ or down‐regulation of 72 and 31 proteins, respectively, of which 18 were tentatively identified by mass spectrometry. The up‐regulation of flavanone‐3‐hydroxylase, alanine aminotransferase, 1‐aminocyclopropane‐1‐carboxylate oxidase, PR‐1 and PR‐10 proteins may partly explain the BTH‐induced resistance against P. sparsa. Other proteins with changes in intensity appear to be involved in, for example, energy metabolism and protein processing. The decline in ATP synthase, triosephosphate isomerase, fructose bisphosphate aldolase and glutamine synthetase suggests that BTH causes significant changes in primary metabolism, which provides one possible explanation for the decreased vegetative growth of foliage and rhizome observed in BTH‐treated plants. |
format |
Text |
author |
HUKKANEN, ANNE KOKKO, HARRI BUCHALA, ANTONY HÄYRINEN, JUKKA KÄRENLAMPI, SIRPA |
author_facet |
HUKKANEN, ANNE KOKKO, HARRI BUCHALA, ANTONY HÄYRINEN, JUKKA KÄRENLAMPI, SIRPA |
author_sort |
HUKKANEN, ANNE |
title |
Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus) |
title_short |
Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus) |
title_full |
Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus) |
title_fullStr |
Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus) |
title_full_unstemmed |
Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus) |
title_sort |
benzothiadiazole affects the leaf proteome in arctic bramble (rubus arcticus) |
publisher |
Blackwell Publishing Ltd |
publishDate |
2008 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640374/ http://www.ncbi.nlm.nih.gov/pubmed/19019008 https://doi.org/10.1111/j.1364-3703.2008.00502.x |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640374/ http://www.ncbi.nlm.nih.gov/pubmed/19019008 http://dx.doi.org/10.1111/j.1364-3703.2008.00502.x |
op_rights |
© 2008 Blackwell publishing Ltd |
op_doi |
https://doi.org/10.1111/j.1364-3703.2008.00502.x |
container_title |
Molecular Plant Pathology |
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9 |
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6 |
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799 |
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808 |
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1766325942846750720 |