Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-ass...
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ftpubmed:oai:pubmedcentral.nih.gov:6629939 2023-05-15T15:27:19+02:00 Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease Marsian, Johanna Hurdiss, Daniel L. Ranson, Neil A. Ritala, Anneli Paley, Richard Cano, Irene Lomonossoff, George P. 2019-07-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/ http://www.ncbi.nlm.nih.gov/pubmed/31354759 https://doi.org/10.3389/fpls.2019.00880 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/ http://www.ncbi.nlm.nih.gov/pubmed/31354759 http://dx.doi.org/10.3389/fpls.2019.00880 Copyright © 2019 Marsian, Hurdiss, Ranson, Ritala, Paley, Cano and Lomonossoff. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Plant Science Text 2019 ftpubmed https://doi.org/10.3389/fpls.2019.00880 2019-08-04T00:43:33Z Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture. Text atlantic cod PubMed Central (PMC) Frontiers in Plant Science 10 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Plant Science |
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Plant Science Marsian, Johanna Hurdiss, Daniel L. Ranson, Neil A. Ritala, Anneli Paley, Richard Cano, Irene Lomonossoff, George P. Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease |
topic_facet |
Plant Science |
description |
Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture. |
format |
Text |
author |
Marsian, Johanna Hurdiss, Daniel L. Ranson, Neil A. Ritala, Anneli Paley, Richard Cano, Irene Lomonossoff, George P. |
author_facet |
Marsian, Johanna Hurdiss, Daniel L. Ranson, Neil A. Ritala, Anneli Paley, Richard Cano, Irene Lomonossoff, George P. |
author_sort |
Marsian, Johanna |
title |
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease |
title_short |
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease |
title_full |
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease |
title_fullStr |
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease |
title_full_unstemmed |
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease |
title_sort |
plant-made nervous necrosis virus-like particles protect fish against disease |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/ http://www.ncbi.nlm.nih.gov/pubmed/31354759 https://doi.org/10.3389/fpls.2019.00880 |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/ http://www.ncbi.nlm.nih.gov/pubmed/31354759 http://dx.doi.org/10.3389/fpls.2019.00880 |
op_rights |
Copyright © 2019 Marsian, Hurdiss, Ranson, Ritala, Paley, Cano and Lomonossoff. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2019.00880 |
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Frontiers in Plant Science |
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10 |
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1766357767551975424 |