Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease

Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-ass...

Full description

Bibliographic Details
Published in:Frontiers in Plant Science
Main Authors: Marsian, Johanna, Hurdiss, Daniel L., Ranson, Neil A., Ritala, Anneli, Paley, Richard, Cano, Irene, Lomonossoff, George P.
Format: Text
Language:English
Published: Frontiers Media S.A. 2019
Subjects:
Plant Science
atlantic cod
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/
http://www.ncbi.nlm.nih.gov/pubmed/31354759
https://doi.org/10.3389/fpls.2019.00880
id ftpubmed:oai:pubmedcentral.nih.gov:6629939
record_format openpolar
spelling ftpubmed:oai:pubmedcentral.nih.gov:6629939 2023-05-15T15:27:19+02:00 Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease Marsian, Johanna Hurdiss, Daniel L. Ranson, Neil A. Ritala, Anneli Paley, Richard Cano, Irene Lomonossoff, George P. 2019-07-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/ http://www.ncbi.nlm.nih.gov/pubmed/31354759 https://doi.org/10.3389/fpls.2019.00880 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/ http://www.ncbi.nlm.nih.gov/pubmed/31354759 http://dx.doi.org/10.3389/fpls.2019.00880 Copyright © 2019 Marsian, Hurdiss, Ranson, Ritala, Paley, Cano and Lomonossoff. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Plant Science Text 2019 ftpubmed https://doi.org/10.3389/fpls.2019.00880 2019-08-04T00:43:33Z Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture. Text atlantic cod PubMed Central (PMC) Frontiers in Plant Science 10
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Plant Science
spellingShingle Plant Science
Marsian, Johanna
Hurdiss, Daniel L.
Ranson, Neil A.
Ritala, Anneli
Paley, Richard
Cano, Irene
Lomonossoff, George P.
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
topic_facet Plant Science
description Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.
format Text
author Marsian, Johanna
Hurdiss, Daniel L.
Ranson, Neil A.
Ritala, Anneli
Paley, Richard
Cano, Irene
Lomonossoff, George P.
author_facet Marsian, Johanna
Hurdiss, Daniel L.
Ranson, Neil A.
Ritala, Anneli
Paley, Richard
Cano, Irene
Lomonossoff, George P.
author_sort Marsian, Johanna
title Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_short Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_full Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_fullStr Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_full_unstemmed Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_sort plant-made nervous necrosis virus-like particles protect fish against disease
publisher Frontiers Media S.A.
publishDate 2019
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/
http://www.ncbi.nlm.nih.gov/pubmed/31354759
https://doi.org/10.3389/fpls.2019.00880
genre atlantic cod
genre_facet atlantic cod
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629939/
http://www.ncbi.nlm.nih.gov/pubmed/31354759
http://dx.doi.org/10.3389/fpls.2019.00880
op_rights Copyright © 2019 Marsian, Hurdiss, Ranson, Ritala, Paley, Cano and Lomonossoff.
http://creativecommons.org/licenses/by/4.0/
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fpls.2019.00880
container_title Frontiers in Plant Science
container_volume 10
_version_ 1766357767551975424

Similar Items