Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments
Sedimentary biofilms comprising microbial communities mediating the anaerobic oxidation of methane are rare. Here, we describe two biofilm communities discovered in sediment cores recovered from Arctic cold seep sites (gas hydrate pingos) in the north-western Barents Sea, characterized by steady met...
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ftpubmed:oai:pubmedcentral.nih.gov:6611871 2023-05-15T14:57:18+02:00 Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments Gründger, Friederike Carrier, Vincent Svenning, Mette M. Panieri, Giuliana Vonnahme, Tobias R. Klasek, Scott Niemann, Helge 2019-07-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611871/ http://www.ncbi.nlm.nih.gov/pubmed/31278352 https://doi.org/10.1038/s41598-019-46209-5 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611871/ http://www.ncbi.nlm.nih.gov/pubmed/31278352 http://dx.doi.org/10.1038/s41598-019-46209-5 © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2019 ftpubmed https://doi.org/10.1038/s41598-019-46209-5 2019-07-21T00:21:38Z Sedimentary biofilms comprising microbial communities mediating the anaerobic oxidation of methane are rare. Here, we describe two biofilm communities discovered in sediment cores recovered from Arctic cold seep sites (gas hydrate pingos) in the north-western Barents Sea, characterized by steady methane fluxes. We found macroscopically visible biofilms in pockets in the sediment matrix at the depth of the sulphate-methane-transition zone. 16S rRNA gene surveys revealed that the microbial community in one of the two biofilms comprised exclusively of putative anaerobic methanotrophic archaea of which ANME-1 was the sole archaeal taxon. The bacterial community consisted of relatives of sulphate-reducing bacteria (SRB) belonging to uncultured Desulfobacteraceae clustering into SEEP-SRB1 (i.e. the typical SRB associated to ANME-1), and members of the atribacterial JS1 clade. Confocal laser scanning microscopy demonstrates that this biofilm is composed of multicellular strands and patches of ANME-1 that are loosely associated with SRB cells, but not tightly connected in aggregates. Our discovery of methanotrophic biofilms in sediment pockets closely associated with methane seeps constitutes a hitherto overlooked and potentially widespread sink for methane and sulphate in marine sediments. Text Arctic Barents Sea PubMed Central (PMC) Arctic Barents Sea Scientific Reports 9 1 |
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Article Gründger, Friederike Carrier, Vincent Svenning, Mette M. Panieri, Giuliana Vonnahme, Tobias R. Klasek, Scott Niemann, Helge Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments |
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Sedimentary biofilms comprising microbial communities mediating the anaerobic oxidation of methane are rare. Here, we describe two biofilm communities discovered in sediment cores recovered from Arctic cold seep sites (gas hydrate pingos) in the north-western Barents Sea, characterized by steady methane fluxes. We found macroscopically visible biofilms in pockets in the sediment matrix at the depth of the sulphate-methane-transition zone. 16S rRNA gene surveys revealed that the microbial community in one of the two biofilms comprised exclusively of putative anaerobic methanotrophic archaea of which ANME-1 was the sole archaeal taxon. The bacterial community consisted of relatives of sulphate-reducing bacteria (SRB) belonging to uncultured Desulfobacteraceae clustering into SEEP-SRB1 (i.e. the typical SRB associated to ANME-1), and members of the atribacterial JS1 clade. Confocal laser scanning microscopy demonstrates that this biofilm is composed of multicellular strands and patches of ANME-1 that are loosely associated with SRB cells, but not tightly connected in aggregates. Our discovery of methanotrophic biofilms in sediment pockets closely associated with methane seeps constitutes a hitherto overlooked and potentially widespread sink for methane and sulphate in marine sediments. |
format |
Text |
author |
Gründger, Friederike Carrier, Vincent Svenning, Mette M. Panieri, Giuliana Vonnahme, Tobias R. Klasek, Scott Niemann, Helge |
author_facet |
Gründger, Friederike Carrier, Vincent Svenning, Mette M. Panieri, Giuliana Vonnahme, Tobias R. Klasek, Scott Niemann, Helge |
author_sort |
Gründger, Friederike |
title |
Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments |
title_short |
Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments |
title_full |
Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments |
title_fullStr |
Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments |
title_full_unstemmed |
Methane-fuelled biofilms predominantly composed of methanotrophic ANME-1 in Arctic gas hydrate-related sediments |
title_sort |
methane-fuelled biofilms predominantly composed of methanotrophic anme-1 in arctic gas hydrate-related sediments |
publisher |
Nature Publishing Group UK |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611871/ http://www.ncbi.nlm.nih.gov/pubmed/31278352 https://doi.org/10.1038/s41598-019-46209-5 |
geographic |
Arctic Barents Sea |
geographic_facet |
Arctic Barents Sea |
genre |
Arctic Barents Sea |
genre_facet |
Arctic Barents Sea |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611871/ http://www.ncbi.nlm.nih.gov/pubmed/31278352 http://dx.doi.org/10.1038/s41598-019-46209-5 |
op_rights |
© The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-46209-5 |
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Scientific Reports |
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