Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

• Published in: Scientific Reports
• Main Authors: Reis-Mansur, Maria Cristina P. P., Cardoso-Rurr, Janine S., Silva, Josemar V. Maiworm Abreu, de Souza, Gabriela Rodrigues, Cardoso, Verônica da Silva, Mansoldo, Felipe Raposo Passos, Pinheiro, Yuri, Schultz, Júnia, Lopez Balottin, Luciene B., da Silva, Antonio Jorge Ribeiro, Lage, Claudia, dos Santos, Elisabete Pereira, Rosado, Alexandre Soares, Vermelho, Alane Beatriz
• Format: Text
• Language: English
• Published: Nature Publishing Group UK 2019
• Subjects: Article; Antarctic; Antarc*
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606617/; http://www.ncbi.nlm.nih.gov/pubmed/31266976; https://doi.org/10.1038/s41598-019-45840-6

The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.