Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity
Cold-active bacteria are currently of great interest in biotechnology, and their genomic and physiological features have been extensively studied. One of the model psychrotolerant bacteria are Psychrobacter spp. Analysis of Arctic psychrophilic Psychrobacter sp. DAB_AL32B genome content provided an...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579772/ http://www.ncbi.nlm.nih.gov/pubmed/30448872 https://doi.org/10.1007/s00203-018-1595-y |
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ftpubmed:oai:pubmedcentral.nih.gov:6579772 2023-05-15T14:55:14+02:00 Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity Ciok, Anna Dziewit, Lukasz 2018-11-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579772/ http://www.ncbi.nlm.nih.gov/pubmed/30448872 https://doi.org/10.1007/s00203-018-1595-y en eng Springer Berlin Heidelberg http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579772/ http://www.ncbi.nlm.nih.gov/pubmed/30448872 http://dx.doi.org/10.1007/s00203-018-1595-y © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. CC-BY Original Paper Text 2018 ftpubmed https://doi.org/10.1007/s00203-018-1595-y 2019-07-07T00:45:10Z Cold-active bacteria are currently of great interest in biotechnology, and their genomic and physiological features have been extensively studied. One of the model psychrotolerant bacteria are Psychrobacter spp. Analysis of Arctic psychrophilic Psychrobacter sp. DAB_AL32B genome content provided an insight into its overall stress response, and genes conferring protection against various life-limiting factors (i.e., low temperature, increased ultraviolet radiation, oxidative stress and osmotic pressure) were recognized and described. Moreover, it was revealed that the strain carries a large plasmid pP32BP2. Its replication system was used for the construction of two novel shuttle vectors (pPS-NR—Psychrobacter-Escherichia coli-specific plasmid and pPS-BR—Psychrobacter-various Proteobacteria-specific plasmid) of an increased carrying capacity, which may be used for genetic engineering of Psychrobacter spp. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00203-018-1595-y) contains supplementary material, which is available to authorized users. Text Arctic PubMed Central (PMC) Arctic Archives of Microbiology 201 5 559 569 |
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Original Paper |
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Original Paper Ciok, Anna Dziewit, Lukasz Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity |
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Original Paper |
description |
Cold-active bacteria are currently of great interest in biotechnology, and their genomic and physiological features have been extensively studied. One of the model psychrotolerant bacteria are Psychrobacter spp. Analysis of Arctic psychrophilic Psychrobacter sp. DAB_AL32B genome content provided an insight into its overall stress response, and genes conferring protection against various life-limiting factors (i.e., low temperature, increased ultraviolet radiation, oxidative stress and osmotic pressure) were recognized and described. Moreover, it was revealed that the strain carries a large plasmid pP32BP2. Its replication system was used for the construction of two novel shuttle vectors (pPS-NR—Psychrobacter-Escherichia coli-specific plasmid and pPS-BR—Psychrobacter-various Proteobacteria-specific plasmid) of an increased carrying capacity, which may be used for genetic engineering of Psychrobacter spp. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00203-018-1595-y) contains supplementary material, which is available to authorized users. |
format |
Text |
author |
Ciok, Anna Dziewit, Lukasz |
author_facet |
Ciok, Anna Dziewit, Lukasz |
author_sort |
Ciok, Anna |
title |
Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity |
title_short |
Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity |
title_full |
Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity |
title_fullStr |
Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity |
title_full_unstemmed |
Exploring the genome of Arctic Psychrobacter sp. DAB_AL32B and construction of novel Psychrobacter-specific cloning vectors of an increased carrying capacity |
title_sort |
exploring the genome of arctic psychrobacter sp. dab_al32b and construction of novel psychrobacter-specific cloning vectors of an increased carrying capacity |
publisher |
Springer Berlin Heidelberg |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579772/ http://www.ncbi.nlm.nih.gov/pubmed/30448872 https://doi.org/10.1007/s00203-018-1595-y |
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Arctic |
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Arctic |
genre |
Arctic |
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Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579772/ http://www.ncbi.nlm.nih.gov/pubmed/30448872 http://dx.doi.org/10.1007/s00203-018-1595-y |
op_rights |
© The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
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CC-BY |
op_doi |
https://doi.org/10.1007/s00203-018-1595-y |
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Archives of Microbiology |
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201 |
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5 |
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559 |
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569 |
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