Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference?
The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique a...
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ftpubmed:oai:pubmedcentral.nih.gov:6554325 2023-05-15T15:36:40+02:00 Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? Marsili, Letizia Di Guardo, Giovanni Mazzariol, Sandro Casini, Silvia 2019-05-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554325/ http://www.ncbi.nlm.nih.gov/pubmed/31214183 https://doi.org/10.3389/fimmu.2019.01219 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554325/ http://www.ncbi.nlm.nih.gov/pubmed/31214183 http://dx.doi.org/10.3389/fimmu.2019.01219 Copyright © 2019 Marsili, Di Guardo, Mazzariol and Casini. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Immunology Text 2019 ftpubmed https://doi.org/10.3389/fimmu.2019.01219 2019-06-23T00:17:32Z The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique and to outline possible variations in MICA expression linked to different ecological and biological factors, while also investigating MICA expression after in vitro treatments with different contaminants. Free-ranging or stranded specimens of cetaceans were sampled in the Sea of Cortez (Mexico) (Balaenoptera edeni, Delphinus capensis, and Orcinus orca) and in the Mediterranean Sea (Balaenoptera physalus, Physeter macrocephalus, Tursiops truncatus, and Stenella coeruleoalba). Cell cultures were treated with an OC mixture, flame retardants, PAHs, MeHg, and BPA. The three species from the Sea of Cortez showed higher basal activity of MICA and lower levels of DDTs and PCBs than the Mediterranean species. A Pearson's linear coefficient equal to −0.45 also confirmed this tendency to have high levels of MICA and low total OC levels. Treatment of cultured fibroblasts with different contaminants mostly resulted in the upregulation of MICA protein expression by at least one treatment dose; downregulation was also found in some species or treatments. MICA alteration indicates a state of stress of the organism and a modification of the immune system's response and can be proposed as a non-invasive immunological marker that can be measured in skin biopsy samples, thus offering a good alternative to blood measurements. Text Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus PubMed Central (PMC) Frontiers in Immunology 10 |
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Immunology |
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Immunology Marsili, Letizia Di Guardo, Giovanni Mazzariol, Sandro Casini, Silvia Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
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Immunology |
description |
The aim of this study was to evaluate the expression of Major histocompatibility complex (MHC) class I chain-related protein A (MICA) in fibroblast cell cultures of cetaceans (skin biopsies of free-ranging specimens and skin samples of freshly stranded cetaceans) by an immunofluorescence technique and to outline possible variations in MICA expression linked to different ecological and biological factors, while also investigating MICA expression after in vitro treatments with different contaminants. Free-ranging or stranded specimens of cetaceans were sampled in the Sea of Cortez (Mexico) (Balaenoptera edeni, Delphinus capensis, and Orcinus orca) and in the Mediterranean Sea (Balaenoptera physalus, Physeter macrocephalus, Tursiops truncatus, and Stenella coeruleoalba). Cell cultures were treated with an OC mixture, flame retardants, PAHs, MeHg, and BPA. The three species from the Sea of Cortez showed higher basal activity of MICA and lower levels of DDTs and PCBs than the Mediterranean species. A Pearson's linear coefficient equal to −0.45 also confirmed this tendency to have high levels of MICA and low total OC levels. Treatment of cultured fibroblasts with different contaminants mostly resulted in the upregulation of MICA protein expression by at least one treatment dose; downregulation was also found in some species or treatments. MICA alteration indicates a state of stress of the organism and a modification of the immune system's response and can be proposed as a non-invasive immunological marker that can be measured in skin biopsy samples, thus offering a good alternative to blood measurements. |
format |
Text |
author |
Marsili, Letizia Di Guardo, Giovanni Mazzariol, Sandro Casini, Silvia |
author_facet |
Marsili, Letizia Di Guardo, Giovanni Mazzariol, Sandro Casini, Silvia |
author_sort |
Marsili, Letizia |
title |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_short |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_full |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_fullStr |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_full_unstemmed |
Insights Into Cetacean Immunology: Do Ecological and Biological Factors Make the Difference? |
title_sort |
insights into cetacean immunology: do ecological and biological factors make the difference? |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554325/ http://www.ncbi.nlm.nih.gov/pubmed/31214183 https://doi.org/10.3389/fimmu.2019.01219 |
genre |
Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus |
genre_facet |
Balaenoptera physalus Orca Orcinus orca Physeter macrocephalus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554325/ http://www.ncbi.nlm.nih.gov/pubmed/31214183 http://dx.doi.org/10.3389/fimmu.2019.01219 |
op_rights |
Copyright © 2019 Marsili, Di Guardo, Mazzariol and Casini. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2019.01219 |
container_title |
Frontiers in Immunology |
container_volume |
10 |
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1766367032539873280 |