Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein
Many marine species inhabiting icy seawater produce antifreeze proteins (AFPs) to prevent their body fluids from freezing. The sculpin species of the superfamily Cottoidea are widely found from the Arctic to southern hemisphere, some of which are known to express AFP. Here we clarified DNA sequence...
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ftpubmed:oai:pubmedcentral.nih.gov:6523315 2023-05-15T14:48:14+02:00 Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein Yamazaki, Aya Nishimiya, Yoshiyuki Tsuda, Sakae Togashi, Koji Munehara, Hiroyuki 2019-04-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523315/ http://www.ncbi.nlm.nih.gov/pubmed/30959891 https://doi.org/10.3390/biom9040139 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523315/ http://www.ncbi.nlm.nih.gov/pubmed/30959891 http://dx.doi.org/10.3390/biom9040139 © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2019 ftpubmed https://doi.org/10.3390/biom9040139 2019-06-09T00:14:34Z Many marine species inhabiting icy seawater produce antifreeze proteins (AFPs) to prevent their body fluids from freezing. The sculpin species of the superfamily Cottoidea are widely found from the Arctic to southern hemisphere, some of which are known to express AFP. Here we clarified DNA sequence encoding type I AFP for 3 species of 2 families (Cottidae and Agonidae) belonging to Cottoidea. We also examined antifreeze activity for 3 families and 32 species of Cottoidea (Cottidae, Agonidae, and Rhamphocottidae). These fishes were collected in 2013–2015 from the Arctic Ocean, Alaska, Japan. We could identify 8 distinct DNA sequences exhibiting a high similarity to those reported for Myoxocephalus species, suggesting that Cottidae and Agonidae share the same DNA sequence encoding type I AFP. Among the 3 families, Rhamphocottidae that experience a warm current did not show antifreeze activity. The species inhabiting the Arctic Ocean and Northern Japan that often covered with ice floe showed high activity, while those inhabiting Alaska, Southern Japan with a warm current showed low/no activity. These results suggest that Cottoidea acquires type I AFP gene before dividing into Cottidae and Agonidae, and have adapted to each location with optimal antifreeze activity level. Text Arctic Arctic Ocean Alaska PubMed Central (PMC) Arctic Arctic Ocean Pacific Biomolecules 9 4 139 |
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Article Yamazaki, Aya Nishimiya, Yoshiyuki Tsuda, Sakae Togashi, Koji Munehara, Hiroyuki Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
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Article |
description |
Many marine species inhabiting icy seawater produce antifreeze proteins (AFPs) to prevent their body fluids from freezing. The sculpin species of the superfamily Cottoidea are widely found from the Arctic to southern hemisphere, some of which are known to express AFP. Here we clarified DNA sequence encoding type I AFP for 3 species of 2 families (Cottidae and Agonidae) belonging to Cottoidea. We also examined antifreeze activity for 3 families and 32 species of Cottoidea (Cottidae, Agonidae, and Rhamphocottidae). These fishes were collected in 2013–2015 from the Arctic Ocean, Alaska, Japan. We could identify 8 distinct DNA sequences exhibiting a high similarity to those reported for Myoxocephalus species, suggesting that Cottidae and Agonidae share the same DNA sequence encoding type I AFP. Among the 3 families, Rhamphocottidae that experience a warm current did not show antifreeze activity. The species inhabiting the Arctic Ocean and Northern Japan that often covered with ice floe showed high activity, while those inhabiting Alaska, Southern Japan with a warm current showed low/no activity. These results suggest that Cottoidea acquires type I AFP gene before dividing into Cottidae and Agonidae, and have adapted to each location with optimal antifreeze activity level. |
format |
Text |
author |
Yamazaki, Aya Nishimiya, Yoshiyuki Tsuda, Sakae Togashi, Koji Munehara, Hiroyuki |
author_facet |
Yamazaki, Aya Nishimiya, Yoshiyuki Tsuda, Sakae Togashi, Koji Munehara, Hiroyuki |
author_sort |
Yamazaki, Aya |
title |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_short |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_full |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_fullStr |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_full_unstemmed |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_sort |
freeze tolerance in sculpins (pisces; cottoidea) inhabiting north pacific and arctic oceans: antifreeze activity and gene sequences of the antifreeze protein |
publisher |
MDPI |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523315/ http://www.ncbi.nlm.nih.gov/pubmed/30959891 https://doi.org/10.3390/biom9040139 |
geographic |
Arctic Arctic Ocean Pacific |
geographic_facet |
Arctic Arctic Ocean Pacific |
genre |
Arctic Arctic Ocean Alaska |
genre_facet |
Arctic Arctic Ocean Alaska |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523315/ http://www.ncbi.nlm.nih.gov/pubmed/30959891 http://dx.doi.org/10.3390/biom9040139 |
op_rights |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/biom9040139 |
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Biomolecules |
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9 |
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139 |
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