Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery

Antarctic have been suggested as an attractive source for antibiotics discovery and members of Streptomyces genus have historically been studied as natural producers of antimicrobial metabolites. Nonetheless, our knowledge on antibiotic-producing Streptomyces from Antarctic is very limited. In this...

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Published in:Scientific Reports
Main Authors: Núñez-Montero, Kattia, Lamilla, Claudio, Abanto, Michel, Maruyama, Fumito, Jorquera, Milko A., Santos, Andrés, Martinez-Urtaza, Jaime, Barrientos, Leticia
Format: Text
Language:English
Published: Nature Publishing Group UK 2019
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Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522549/
http://www.ncbi.nlm.nih.gov/pubmed/31097761
https://doi.org/10.1038/s41598-019-43960-7
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spelling ftpubmed:oai:pubmedcentral.nih.gov:6522549 2023-05-15T13:50:01+02:00 Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery Núñez-Montero, Kattia Lamilla, Claudio Abanto, Michel Maruyama, Fumito Jorquera, Milko A. Santos, Andrés Martinez-Urtaza, Jaime Barrientos, Leticia 2019-05-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522549/ http://www.ncbi.nlm.nih.gov/pubmed/31097761 https://doi.org/10.1038/s41598-019-43960-7 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522549/ http://www.ncbi.nlm.nih.gov/pubmed/31097761 http://dx.doi.org/10.1038/s41598-019-43960-7 © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2019 ftpubmed https://doi.org/10.1038/s41598-019-43960-7 2019-06-02T00:20:20Z Antarctic have been suggested as an attractive source for antibiotics discovery and members of Streptomyces genus have historically been studied as natural producers of antimicrobial metabolites. Nonetheless, our knowledge on antibiotic-producing Streptomyces from Antarctic is very limited. In this study, the antimicrobial activity of organic extracts from Antarctic Streptomyces strains was evaluated by disk diffusion assays and minimum inhibitory concentration. The strain Streptomyces sp. So13.3 showed the greatest antibiotic activity (MIC = 15.6 μg/mL) against Gram-positive bacteria and growth reduction of Gram‒negative pathogens. The bioactive fraction in the crude extract was revealed by TLC‒bioautography at R(f) = 0.78 with molecular weight between 148 and 624 m/z detected by LC-ESI-MS/MS. The strain So13.3 was taxonomically affiliated as Streptomyces fildesensis. Whole genome sequencing and analysis suggested a 9.47 Mb genome size with 42 predicted biosynthetic gene clusters (BGCs) and 56 putative clusters representing a 22% of total genome content. Interestingly, a large number of them (11 of 42 BGCs and 40 of 56 putative BGCs), did not show similarities with other known BGCs. Our results highlight the potential of the Antarctic Streptomyces strains as a promising source of novel antimicrobials, particularly the strain Streptomyces fildesensis So13.3, which first draft genome is reported in this work. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Scientific Reports 9 1
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Article
spellingShingle Article
Núñez-Montero, Kattia
Lamilla, Claudio
Abanto, Michel
Maruyama, Fumito
Jorquera, Milko A.
Santos, Andrés
Martinez-Urtaza, Jaime
Barrientos, Leticia
Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
topic_facet Article
description Antarctic have been suggested as an attractive source for antibiotics discovery and members of Streptomyces genus have historically been studied as natural producers of antimicrobial metabolites. Nonetheless, our knowledge on antibiotic-producing Streptomyces from Antarctic is very limited. In this study, the antimicrobial activity of organic extracts from Antarctic Streptomyces strains was evaluated by disk diffusion assays and minimum inhibitory concentration. The strain Streptomyces sp. So13.3 showed the greatest antibiotic activity (MIC = 15.6 μg/mL) against Gram-positive bacteria and growth reduction of Gram‒negative pathogens. The bioactive fraction in the crude extract was revealed by TLC‒bioautography at R(f) = 0.78 with molecular weight between 148 and 624 m/z detected by LC-ESI-MS/MS. The strain So13.3 was taxonomically affiliated as Streptomyces fildesensis. Whole genome sequencing and analysis suggested a 9.47 Mb genome size with 42 predicted biosynthetic gene clusters (BGCs) and 56 putative clusters representing a 22% of total genome content. Interestingly, a large number of them (11 of 42 BGCs and 40 of 56 putative BGCs), did not show similarities with other known BGCs. Our results highlight the potential of the Antarctic Streptomyces strains as a promising source of novel antimicrobials, particularly the strain Streptomyces fildesensis So13.3, which first draft genome is reported in this work.
format Text
author Núñez-Montero, Kattia
Lamilla, Claudio
Abanto, Michel
Maruyama, Fumito
Jorquera, Milko A.
Santos, Andrés
Martinez-Urtaza, Jaime
Barrientos, Leticia
author_facet Núñez-Montero, Kattia
Lamilla, Claudio
Abanto, Michel
Maruyama, Fumito
Jorquera, Milko A.
Santos, Andrés
Martinez-Urtaza, Jaime
Barrientos, Leticia
author_sort Núñez-Montero, Kattia
title Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_short Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_full Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_fullStr Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_full_unstemmed Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_sort antarctic streptomyces fildesensis so13.3 strain as a promising source for antimicrobials discovery
publisher Nature Publishing Group UK
publishDate 2019
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522549/
http://www.ncbi.nlm.nih.gov/pubmed/31097761
https://doi.org/10.1038/s41598-019-43960-7
geographic Antarctic
The Antarctic
geographic_facet Antarctic
The Antarctic
genre Antarc*
Antarctic
genre_facet Antarc*
Antarctic
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522549/
http://www.ncbi.nlm.nih.gov/pubmed/31097761
http://dx.doi.org/10.1038/s41598-019-43960-7
op_rights © The Author(s) 2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
op_rightsnorm CC-BY
op_doi https://doi.org/10.1038/s41598-019-43960-7
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