Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis)
Gammaherpesviruses (GHVs) infect many animal species and are associated with lymphoproliferative disorders in some. Previously, we identified several novel GHVs in North American felids; however, a GHV had never been identified in Canada lynx (Lynx canadensis). We, therefore, hypothesized the existe...
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ftpubmed:oai:pubmedcentral.nih.gov:6520957 2023-05-15T17:20:02+02:00 Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis) Hendrikse, Liam D. Kambli, Ankita Kayko, Caroline Canuti, Marta Rodrigues, Bruce Stevens, Brian Vashon, Jennifer Lang, Andrew S. Needle, David B. Troyer, Ryan M. 2019-04-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520957/ http://www.ncbi.nlm.nih.gov/pubmed/31010021 https://doi.org/10.3390/v11040363 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520957/ http://www.ncbi.nlm.nih.gov/pubmed/31010021 http://dx.doi.org/10.3390/v11040363 © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2019 ftpubmed https://doi.org/10.3390/v11040363 2019-06-09T00:13:27Z Gammaherpesviruses (GHVs) infect many animal species and are associated with lymphoproliferative disorders in some. Previously, we identified several novel GHVs in North American felids; however, a GHV had never been identified in Canada lynx (Lynx canadensis). We, therefore, hypothesized the existence of an unidentified GHV in lynx. Using degenerate nested and subsequently virus-specific PCR, we amplified and sequenced 3.4 kb of DNA from a novel GHV in lynx, which we named Lynx canadensis gammaherpesvirus 1 (LcaGHV1). Phylogenetic analysis determined that LcaGHV1 is a distinct GHV species belonging to the genus Percavirus. We then estimated the prevalence of LcaGHV1 in lynx by developing a PCR-based assay and detected LcaGHV1 DNA in 36% (95% CI: 22–53%) of lynx spleen DNA samples from Maine, USA and 17% (95% CI: 8–31%) from Newfoundland, Canada. The LcaGHV1 DNA sequences from Maine and Newfoundland lynx were nearly identical to each other (two nucleotide substitutions in 3.4 kb), suggesting that the unique lynx subspecies present on the island of Newfoundland (Lynx canadensis subsolanus) is infected with virus that very closely resembles virus found in mainland lynx. The potential ecologic and pathologic consequences of this novel virus for Canada lynx populations warrant further study. Text Newfoundland Lynx PubMed Central (PMC) Canada Viruses 11 4 363 |
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Article Hendrikse, Liam D. Kambli, Ankita Kayko, Caroline Canuti, Marta Rodrigues, Bruce Stevens, Brian Vashon, Jennifer Lang, Andrew S. Needle, David B. Troyer, Ryan M. Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis) |
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Gammaherpesviruses (GHVs) infect many animal species and are associated with lymphoproliferative disorders in some. Previously, we identified several novel GHVs in North American felids; however, a GHV had never been identified in Canada lynx (Lynx canadensis). We, therefore, hypothesized the existence of an unidentified GHV in lynx. Using degenerate nested and subsequently virus-specific PCR, we amplified and sequenced 3.4 kb of DNA from a novel GHV in lynx, which we named Lynx canadensis gammaherpesvirus 1 (LcaGHV1). Phylogenetic analysis determined that LcaGHV1 is a distinct GHV species belonging to the genus Percavirus. We then estimated the prevalence of LcaGHV1 in lynx by developing a PCR-based assay and detected LcaGHV1 DNA in 36% (95% CI: 22–53%) of lynx spleen DNA samples from Maine, USA and 17% (95% CI: 8–31%) from Newfoundland, Canada. The LcaGHV1 DNA sequences from Maine and Newfoundland lynx were nearly identical to each other (two nucleotide substitutions in 3.4 kb), suggesting that the unique lynx subspecies present on the island of Newfoundland (Lynx canadensis subsolanus) is infected with virus that very closely resembles virus found in mainland lynx. The potential ecologic and pathologic consequences of this novel virus for Canada lynx populations warrant further study. |
format |
Text |
author |
Hendrikse, Liam D. Kambli, Ankita Kayko, Caroline Canuti, Marta Rodrigues, Bruce Stevens, Brian Vashon, Jennifer Lang, Andrew S. Needle, David B. Troyer, Ryan M. |
author_facet |
Hendrikse, Liam D. Kambli, Ankita Kayko, Caroline Canuti, Marta Rodrigues, Bruce Stevens, Brian Vashon, Jennifer Lang, Andrew S. Needle, David B. Troyer, Ryan M. |
author_sort |
Hendrikse, Liam D. |
title |
Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_short |
Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_full |
Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_fullStr |
Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_full_unstemmed |
Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_sort |
identification of a novel gammaherpesvirus in canada lynx (lynx canadensis) |
publisher |
MDPI |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520957/ http://www.ncbi.nlm.nih.gov/pubmed/31010021 https://doi.org/10.3390/v11040363 |
geographic |
Canada |
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Canada |
genre |
Newfoundland Lynx |
genre_facet |
Newfoundland Lynx |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520957/ http://www.ncbi.nlm.nih.gov/pubmed/31010021 http://dx.doi.org/10.3390/v11040363 |
op_rights |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/v11040363 |
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Viruses |
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11 |
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363 |
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