Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines
The γ-carbonic anhydrases (CAs, EC 4.2.1.1) present in the Antarctic marine bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea, herein referred to as PhaCA and CpsCA, respectively, were investigated for their activation with a panel of 24 amino acids and amines. Both bacteria are...
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ftpubmed:oai:pubmedcentral.nih.gov:6520686 2023-05-15T13:59:39+02:00 Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines Angeli, Andrea Del Prete, Sonia Osman, Sameh M. AlOthman, Zeid Donald, William A. Capasso, Clemente Supuran, Claudiu T. 2019-04-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520686/ http://www.ncbi.nlm.nih.gov/pubmed/31013612 https://doi.org/10.3390/md17040238 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520686/ http://www.ncbi.nlm.nih.gov/pubmed/31013612 http://dx.doi.org/10.3390/md17040238 © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2019 ftpubmed https://doi.org/10.3390/md17040238 2019-06-09T00:13:18Z The γ-carbonic anhydrases (CAs, EC 4.2.1.1) present in the Antarctic marine bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea, herein referred to as PhaCA and CpsCA, respectively, were investigated for their activation with a panel of 24 amino acids and amines. Both bacteria are considered Antarctic models for the investigation of photosynthetic and metabolic pathways in organisms adapted to live in cold seawater. PhaCA was much more sensitive to activation by these compounds compared to the genetically related enzyme CpsCA. The most effective PhaCA activators were d-Phe, l-/d-DOPA, l-Tyr and 2-pyridyl-methylamine, with the activation constant K(A) values of 0.72–3.27 µM. d-His, l-Trp, d-Tyr, histamine, dopamine, serotonin anddicarboxylic amino acids were also effective activators of PhaCA, with K(A) values of 6.48–9.85 µM. CpsCA was activated by d-Phe, d-DOPA, l-Trp, l-/d-Tyr, 4-amino-l-Phe, histamine, 2-pyridyl-methylamine and l-/d-Glu with K(A) values of 11.2–24.4 µM. The most effective CpsCA activator was l-DOPA (K(A) of 4.79 µM). Given that modulators of CAs from Antarctic bacteria have not been identified and investigated in detail for their metabolic roles to date, this research sheds some light on these poorly understood processes. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Marine Drugs 17 4 238 |
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Article Angeli, Andrea Del Prete, Sonia Osman, Sameh M. AlOthman, Zeid Donald, William A. Capasso, Clemente Supuran, Claudiu T. Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines |
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Article |
description |
The γ-carbonic anhydrases (CAs, EC 4.2.1.1) present in the Antarctic marine bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea, herein referred to as PhaCA and CpsCA, respectively, were investigated for their activation with a panel of 24 amino acids and amines. Both bacteria are considered Antarctic models for the investigation of photosynthetic and metabolic pathways in organisms adapted to live in cold seawater. PhaCA was much more sensitive to activation by these compounds compared to the genetically related enzyme CpsCA. The most effective PhaCA activators were d-Phe, l-/d-DOPA, l-Tyr and 2-pyridyl-methylamine, with the activation constant K(A) values of 0.72–3.27 µM. d-His, l-Trp, d-Tyr, histamine, dopamine, serotonin anddicarboxylic amino acids were also effective activators of PhaCA, with K(A) values of 6.48–9.85 µM. CpsCA was activated by d-Phe, d-DOPA, l-Trp, l-/d-Tyr, 4-amino-l-Phe, histamine, 2-pyridyl-methylamine and l-/d-Glu with K(A) values of 11.2–24.4 µM. The most effective CpsCA activator was l-DOPA (K(A) of 4.79 µM). Given that modulators of CAs from Antarctic bacteria have not been identified and investigated in detail for their metabolic roles to date, this research sheds some light on these poorly understood processes. |
format |
Text |
author |
Angeli, Andrea Del Prete, Sonia Osman, Sameh M. AlOthman, Zeid Donald, William A. Capasso, Clemente Supuran, Claudiu T. |
author_facet |
Angeli, Andrea Del Prete, Sonia Osman, Sameh M. AlOthman, Zeid Donald, William A. Capasso, Clemente Supuran, Claudiu T. |
author_sort |
Angeli, Andrea |
title |
Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines |
title_short |
Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines |
title_full |
Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines |
title_fullStr |
Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines |
title_full_unstemmed |
Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines |
title_sort |
activation studies of the γ-carbonic anhydrases from the antarctic marine bacteria pseudoalteromonas haloplanktis and colwellia psychrerythraea with amino acids and amines |
publisher |
MDPI |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520686/ http://www.ncbi.nlm.nih.gov/pubmed/31013612 https://doi.org/10.3390/md17040238 |
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Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520686/ http://www.ncbi.nlm.nih.gov/pubmed/31013612 http://dx.doi.org/10.3390/md17040238 |
op_rights |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md17040238 |
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Marine Drugs |
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17 |
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4 |
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238 |
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1766268324237279232 |