Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles
Purpose: Here, we present the successful preparation of a highly efficient gallic acid resin grafted with magnetic nanoparticles (MNPs) and containing a branched brush polymeric shell. Methods: Using a convenient co-precipitation method, we prepared Fe(3)O(4) nanoparticles stabilized by citric acid....
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ftpubmed:oai:pubmedcentral.nih.gov:6504639 2023-05-15T13:52:23+02:00 Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles SreeHarsha, Nagaraja Ghorpade, Ravindra V Alzahrani, Abdullah Mossa Al-Dhubiab, Bandar E Venugopala, Katharigatta N 2019-05-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504639/ https://doi.org/10.2147/IJN.S203547 en eng Dove http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504639/ http://dx.doi.org/10.2147/IJN.S203547 © 2019 SreeHarsha et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). CC-BY-NC Original Research Text 2019 ftpubmed https://doi.org/10.2147/IJN.S203547 2019-05-26T00:15:13Z Purpose: Here, we present the successful preparation of a highly efficient gallic acid resin grafted with magnetic nanoparticles (MNPs) and containing a branched brush polymeric shell. Methods: Using a convenient co-precipitation method, we prepared Fe(3)O(4) nanoparticles stabilized by citric acid. These nanoparticles underwent further silica modification and amino functionalization followed by gallic acid functionalization on their surface. Under alkaline conditions, we used a condensation reaction that combined formaldehyde and gallic, to graft the gallic acid−formaldehyde resin on the surface. We then evaluated the polymer-grafted MNPs to assay the Candida Antarctica B lipase(Cal-B) immobilization via physical adsorption. Conclusion: Furthermore, during optimization of parameters that defined conditions of immobilization, we found that the optimum immobilization was achieved in 15 mins. Also, optimal immobilization temperature and pH were 38ºC and 7.5, respectively. In addition, the reusability study of immobilized lipase polymer-grafted MNPs was done by isolating the MNPs from the reaction medium using magnetic separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention. Text Antarc* Antarctica PubMed Central (PMC) International Journal of Nanomedicine Volume 14 3235 3244 |
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Original Research SreeHarsha, Nagaraja Ghorpade, Ravindra V Alzahrani, Abdullah Mossa Al-Dhubiab, Bandar E Venugopala, Katharigatta N Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles |
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Original Research |
description |
Purpose: Here, we present the successful preparation of a highly efficient gallic acid resin grafted with magnetic nanoparticles (MNPs) and containing a branched brush polymeric shell. Methods: Using a convenient co-precipitation method, we prepared Fe(3)O(4) nanoparticles stabilized by citric acid. These nanoparticles underwent further silica modification and amino functionalization followed by gallic acid functionalization on their surface. Under alkaline conditions, we used a condensation reaction that combined formaldehyde and gallic, to graft the gallic acid−formaldehyde resin on the surface. We then evaluated the polymer-grafted MNPs to assay the Candida Antarctica B lipase(Cal-B) immobilization via physical adsorption. Conclusion: Furthermore, during optimization of parameters that defined conditions of immobilization, we found that the optimum immobilization was achieved in 15 mins. Also, optimal immobilization temperature and pH were 38ºC and 7.5, respectively. In addition, the reusability study of immobilized lipase polymer-grafted MNPs was done by isolating the MNPs from the reaction medium using magnetic separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention. |
format |
Text |
author |
SreeHarsha, Nagaraja Ghorpade, Ravindra V Alzahrani, Abdullah Mossa Al-Dhubiab, Bandar E Venugopala, Katharigatta N |
author_facet |
SreeHarsha, Nagaraja Ghorpade, Ravindra V Alzahrani, Abdullah Mossa Al-Dhubiab, Bandar E Venugopala, Katharigatta N |
author_sort |
SreeHarsha, Nagaraja |
title |
Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles |
title_short |
Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles |
title_full |
Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles |
title_fullStr |
Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles |
title_full_unstemmed |
Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles |
title_sort |
immobilization studies of candida antarctica lipase b on gallic acid resin-grafted magnetic iron oxide nanoparticles |
publisher |
Dove |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504639/ https://doi.org/10.2147/IJN.S203547 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504639/ http://dx.doi.org/10.2147/IJN.S203547 |
op_rights |
© 2019 SreeHarsha et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
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CC-BY-NC |
op_doi |
https://doi.org/10.2147/IJN.S203547 |
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International Journal of Nanomedicine |
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Volume 14 |
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3235 |
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3244 |
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1766256663869784064 |