Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing
This study comprises the first attempt to describe the planktonic bacterial communities of lakes from Byers Peninsula, one of the most significant limnological districts in the Maritime Antarctica, leveraging next-generation sequencing (NGS) technologies. For the survey, we selected 7 lakes covering...
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ftpubmed:oai:pubmedcentral.nih.gov:6503055 2023-05-15T13:52:23+02:00 Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing Picazo, Antonio Rochera, Carlos Villaescusa, Juan Antonio Miralles-Lorenzo, Javier Velázquez, David Quesada, Antonio Camacho, Antonio 2019-04-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503055/ http://www.ncbi.nlm.nih.gov/pubmed/31114558 https://doi.org/10.3389/fmicb.2019.00908 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503055/ http://www.ncbi.nlm.nih.gov/pubmed/31114558 http://dx.doi.org/10.3389/fmicb.2019.00908 Copyright © 2019 Picazo, Rochera, Villaescusa, Miralles-Lorenzo, Velázquez, Quesada and Camacho. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2019 ftpubmed https://doi.org/10.3389/fmicb.2019.00908 2019-05-26T00:14:25Z This study comprises the first attempt to describe the planktonic bacterial communities of lakes from Byers Peninsula, one of the most significant limnological districts in the Maritime Antarctica, leveraging next-generation sequencing (NGS) technologies. For the survey, we selected 7 lakes covering the environmental gradient from inland to coastal lakes, some of them sampled both in surface and deep waters. Analysis provided just over 85,000 high quality sequences that were clustered into 864 unique Zero-radius Operational Taxonomic Units (ZOTUs) (i.e., 100% sequence similarity). Yet, several taxonomic uncertainties remained in the analysis likely suggesting the occurrence of local bacterial adaptations. The survey showed the dominance of the phyla Proteobacteria and Bacteroidetes. Among the former, the Gammaproteobacteria class, more specifically the order Betaproteobacteriales, was the dominant group, which seems to be a common trend in nutrient-limited Antarctic lakes. Most of the families and genera ubiquitously detected belonging to this class are indeed typical from ultra-oligotrophic environments, and commonly described as diazotrophs. On the other hand, among the members of the phylum Bacteroidetes, genera such as Flavobacterium were abundant in some of the shallowest lakes, thus demonstrating that also benthic and sediment-associated bacteria contributed to water bacterial assemblages. Ordination analyses sorted bacterial assemblages mainly based on the environmental gradients of nutrient availability and conductivity i.e., salinity. However, transient bacterial associations, that included the groups Clostridiaceae and Chloroflexi, also occurred as being forced by other drivers such as the influence of the nearby fauna and by the airborne microorganisms. As we intended, our NGS-based approach has provided a much greater resolution compared to the previous studies conducted in the area and confirmed to a large extent the previously obtained patterns, thus reinforcing the view of Byers as a hotspot of ... Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic Byers ENVELOPE(-60.283,-60.283,-63.900,-63.900) Byers peninsula ENVELOPE(-61.066,-61.066,-62.633,-62.633) Frontiers in Microbiology 10 |
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PubMed Central (PMC) |
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language |
English |
topic |
Microbiology |
spellingShingle |
Microbiology Picazo, Antonio Rochera, Carlos Villaescusa, Juan Antonio Miralles-Lorenzo, Javier Velázquez, David Quesada, Antonio Camacho, Antonio Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
topic_facet |
Microbiology |
description |
This study comprises the first attempt to describe the planktonic bacterial communities of lakes from Byers Peninsula, one of the most significant limnological districts in the Maritime Antarctica, leveraging next-generation sequencing (NGS) technologies. For the survey, we selected 7 lakes covering the environmental gradient from inland to coastal lakes, some of them sampled both in surface and deep waters. Analysis provided just over 85,000 high quality sequences that were clustered into 864 unique Zero-radius Operational Taxonomic Units (ZOTUs) (i.e., 100% sequence similarity). Yet, several taxonomic uncertainties remained in the analysis likely suggesting the occurrence of local bacterial adaptations. The survey showed the dominance of the phyla Proteobacteria and Bacteroidetes. Among the former, the Gammaproteobacteria class, more specifically the order Betaproteobacteriales, was the dominant group, which seems to be a common trend in nutrient-limited Antarctic lakes. Most of the families and genera ubiquitously detected belonging to this class are indeed typical from ultra-oligotrophic environments, and commonly described as diazotrophs. On the other hand, among the members of the phylum Bacteroidetes, genera such as Flavobacterium were abundant in some of the shallowest lakes, thus demonstrating that also benthic and sediment-associated bacteria contributed to water bacterial assemblages. Ordination analyses sorted bacterial assemblages mainly based on the environmental gradients of nutrient availability and conductivity i.e., salinity. However, transient bacterial associations, that included the groups Clostridiaceae and Chloroflexi, also occurred as being forced by other drivers such as the influence of the nearby fauna and by the airborne microorganisms. As we intended, our NGS-based approach has provided a much greater resolution compared to the previous studies conducted in the area and confirmed to a large extent the previously obtained patterns, thus reinforcing the view of Byers as a hotspot of ... |
format |
Text |
author |
Picazo, Antonio Rochera, Carlos Villaescusa, Juan Antonio Miralles-Lorenzo, Javier Velázquez, David Quesada, Antonio Camacho, Antonio |
author_facet |
Picazo, Antonio Rochera, Carlos Villaescusa, Juan Antonio Miralles-Lorenzo, Javier Velázquez, David Quesada, Antonio Camacho, Antonio |
author_sort |
Picazo, Antonio |
title |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_short |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_full |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_fullStr |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_full_unstemmed |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_sort |
bacterioplankton community composition along environmental gradients in lakes from byers peninsula (maritime antarctica) as determined by next-generation sequencing |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503055/ http://www.ncbi.nlm.nih.gov/pubmed/31114558 https://doi.org/10.3389/fmicb.2019.00908 |
long_lat |
ENVELOPE(-60.283,-60.283,-63.900,-63.900) ENVELOPE(-61.066,-61.066,-62.633,-62.633) |
geographic |
Antarctic Byers Byers peninsula |
geographic_facet |
Antarctic Byers Byers peninsula |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503055/ http://www.ncbi.nlm.nih.gov/pubmed/31114558 http://dx.doi.org/10.3389/fmicb.2019.00908 |
op_rights |
Copyright © 2019 Picazo, Rochera, Villaescusa, Miralles-Lorenzo, Velázquez, Quesada and Camacho. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2019.00908 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766256663662166016 |