Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis
The tissue culture of Phaseolus vulgaris has always been considered difficult. Its regenerative capacity and response to culture conditions are highly genotype-dependent and hamper the application of genetic engineering. The objective of this study was to develop a repeatable technique for organogen...
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ftpubmed:oai:pubmedcentral.nih.gov:6472361 2023-05-15T17:06:38+02:00 Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis Hnatuszko-Konka, Katarzyna Kowalczyk, Tomasz Gerszberg, Aneta Glińska, Sława Grzegorczyk-Karolak, Izabela 2019-04-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472361/ http://www.ncbi.nlm.nih.gov/pubmed/31000799 https://doi.org/10.1038/s41598-019-42723-8 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472361/ http://www.ncbi.nlm.nih.gov/pubmed/31000799 http://dx.doi.org/10.1038/s41598-019-42723-8 © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2019 ftpubmed https://doi.org/10.1038/s41598-019-42723-8 2019-04-28T00:24:43Z The tissue culture of Phaseolus vulgaris has always been considered difficult. Its regenerative capacity and response to culture conditions are highly genotype-dependent and hamper the application of genetic engineering. The objective of this study was to develop a repeatable technique for organogenic bud induction from selected explants of the common bean. Epicotyls and hypocotyls of six cultivars were investigated to determine the effect of the genotype, and four variants of two basal media (Murashige-Skoog and Gamborg) were tested. The composition of these medium variants was based on the published data suggesting the most universal medium compounds that show the advantage of being applicable to different cultivars. As a result, the common bean epicotyls showed undisputed regeneration superiority over the hypocotyls. Moreover, a well-known observation was confirmed, namely that common bean regeneration is cultivar-specific or at least specific to the cluster of related cultivars. However, efficient regeneration was achieved most often when the epicotyls were incubated on the MS or B5 media amended with AgNO(3) and BAP. Additionally, the positive synergistic influence of activated charcoal and silver nitrate on bud formation was demonstrated. The highest values of the epicotyl in vitro response for the common bean cultivars could be presented as follows: Czerwona (70.00%) > Goldpantera (58.89%) and Ibiza (58.89%) > Plus (55.56%) > Laponia (50.56%) > Złota Saxa (46.11%). Text Laponia PubMed Central (PMC) Scientific Reports 9 1 |
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Article Hnatuszko-Konka, Katarzyna Kowalczyk, Tomasz Gerszberg, Aneta Glińska, Sława Grzegorczyk-Karolak, Izabela Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis |
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description |
The tissue culture of Phaseolus vulgaris has always been considered difficult. Its regenerative capacity and response to culture conditions are highly genotype-dependent and hamper the application of genetic engineering. The objective of this study was to develop a repeatable technique for organogenic bud induction from selected explants of the common bean. Epicotyls and hypocotyls of six cultivars were investigated to determine the effect of the genotype, and four variants of two basal media (Murashige-Skoog and Gamborg) were tested. The composition of these medium variants was based on the published data suggesting the most universal medium compounds that show the advantage of being applicable to different cultivars. As a result, the common bean epicotyls showed undisputed regeneration superiority over the hypocotyls. Moreover, a well-known observation was confirmed, namely that common bean regeneration is cultivar-specific or at least specific to the cluster of related cultivars. However, efficient regeneration was achieved most often when the epicotyls were incubated on the MS or B5 media amended with AgNO(3) and BAP. Additionally, the positive synergistic influence of activated charcoal and silver nitrate on bud formation was demonstrated. The highest values of the epicotyl in vitro response for the common bean cultivars could be presented as follows: Czerwona (70.00%) > Goldpantera (58.89%) and Ibiza (58.89%) > Plus (55.56%) > Laponia (50.56%) > Złota Saxa (46.11%). |
format |
Text |
author |
Hnatuszko-Konka, Katarzyna Kowalczyk, Tomasz Gerszberg, Aneta Glińska, Sława Grzegorczyk-Karolak, Izabela |
author_facet |
Hnatuszko-Konka, Katarzyna Kowalczyk, Tomasz Gerszberg, Aneta Glińska, Sława Grzegorczyk-Karolak, Izabela |
author_sort |
Hnatuszko-Konka, Katarzyna |
title |
Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis |
title_short |
Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis |
title_full |
Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis |
title_fullStr |
Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis |
title_full_unstemmed |
Regeneration of Phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis |
title_sort |
regeneration of phaseolus vulgaris from epicotyls and hypocotyls via direct organogenesis |
publisher |
Nature Publishing Group UK |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472361/ http://www.ncbi.nlm.nih.gov/pubmed/31000799 https://doi.org/10.1038/s41598-019-42723-8 |
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Laponia |
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Laponia |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472361/ http://www.ncbi.nlm.nih.gov/pubmed/31000799 http://dx.doi.org/10.1038/s41598-019-42723-8 |
op_rights |
© The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-42723-8 |
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Scientific Reports |
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